Restriction map for pF1KB6142
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BbeI        
BbvCI       BfaI        BfrI        BglII       BlnI        
BsiWI       BsmBI       Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HpaI        KasI        KpnI        MaeI        
MfeI        MflI        MluI        MunI        NarI        
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tsp45I      XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         889  CGCG                 CG^CG
AccIII        713  TCCGGA               T^CCGGA
AgeI           81  ACCGGT               A^CCGGT
AlwI          367  GATCC                GGATC(4/5)
AspI         1270  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1832  TGGCCA               TGG^CCA
BbrPI        1674  CACGTG               CAC^GTG
BcgI         1292  GCAATGGGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         463  GGATAC               GTATCC(6/5)
BclI          395  TGATCA               T^GATCA
BseAI         713  TCCGGA               T^CCGGA
BsmI         1137  GCATTC               GAATGC(1/-1)
BspEI         713  TCCGGA               T^CCGGA
BspMI        1345  GCAGGT               ACCTGC(4/8)
BsrDI        1292  GCAATG               GCAATG(2/0)
BstUI         889  CGCG                 CG^CG
DraIII       1399  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           32  CGGCCG               C^GGCCG
EclXI          32  CGGCCG               C^GGCCG
Eco52I         32  CGGCCG               C^GGCCG
EcoNI         219  CCTTGCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          395  TGATCA               T^GATCA
HincII       1537  GTCAAC               GT[CT]^[GA]AC
HindII       1537  GTCAAC               GT[CT]^[GA]AC
HindIII       175  AAGCTT               A^AGCTT
KspI          888  CCGCGG               CCGC^GG
MluNI        1832  TGGCCA               TGG^CCA
MroI          713  TCCGGA               T^CCGGA
MscI         1832  TGGCCA               TGG^CCA
MvnI          889  CGCG                 CG^CG
NaeI         1610  GCCGGC               GCC^GGC
NdeI          769  CATATG               CA^TATG
NgoMIV       1610  GCCGGC               G^CCGGC
NotI           31  GCGGCCGC             GC^GGCCGC
PflFI        1270  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI          81  ACCGGT               A^CCGGT
PmaCI        1674  CACGTG               CAC^GTG
PmlI         1674  CACGTG               CAC^GTG
SacI          847  GAGCTC               GAGCT^C
SacII         888  CCGCGG               CCGC^GG
SexAI        1802  ACCAGGT              A^CC[AT]GGT
SmaI          842  CCCGGG               CCC^GGG
StuI          752  AGGCCT               AGG^CCT
Tth111I      1270  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         842  CCCGGG               C^CCGGG
XmaI          842  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI         1309  GAATTT               [GA]^AATT[CT]
             1494  AAATTT              
AflIII        911  ACGTGT               A^C[GA][CT]GT
             1673  ACACGT              
AlwNI         259  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1228  CAGCATCTG           
ApaI          807  GGGCCC               GGGCC^C
              838  GGGCCC              
ApoI         1309  GAATTT               [GA]^AATT[CT]
             1494  AAATTT              
AspHI         847  GAGCTC               G[AT]GC[AT]^C
             1240  GTGCTC              
BlpI           73  GCTGAGC              GC^T[ACGT]AGC
             1605  GCTGAGC             
Bpu1102I       73  GCTGAGC              GC^T[ACGT]AGC
             1605  GCTGAGC             
BsaAI         910  TACGTG               [CT]AC^GT[GA]
             1674  CACGTG              
BsaBI         348  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              783  GATTGACATC          
BsaI          272  GAGACC               GGTCTC(1/5)
             1556  GAGACC              
BsaWI          81  ACCGGT               [AT]^CCGG[AT]
              713  TCCGGA              
BsiEI          32  CGGCCG               CG[GA][CT]^CG
               79  CGACCG              
BsiHKAI       847  GAGCTC               G[AT]GC[AT]^C
             1240  GTGCTC              
BspHI         734  TCATGA               T^CATGA
             1661  TCATGA              
CelII          73  GCTGAGC              GC^T[ACGT]AGC
             1605  GCTGAGC             
HaeII         796  AGCGCC               [GA]GCGC^[CT]
             1729  AGCGCC              
MaeII         911  ACGT                 A^CGT
             1675  ACGT                
MamI          348  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              783  GATTGACATC          
NcoI          863  CCATGG               C^CATGG
             1352  CCATGG              
PleI         1630  GACTC                GAGTC(4/5)
              167  GAGTC               
PstI          856  CTGCAG               CTGCA^G
             1151  CTGCAG              
RcaI          734  TCATGA               T^CATGA
             1661  TCATGA              
SapI         1861  GAAGAGC              GCTCTTC(1/4)
             1040  GCTCTTC             
TfiI           93  GAATC                G^A[AT]TC
             1394  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
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