Restriction map for pF1KB6142
 (Linear) (Six-base) MAPSORT of: KIBB6142.seq  Check: 8034  from: 1  to: 1914



KIBB6142.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1336    1914 
   Size:       1336     578
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     467     549    1047    1771    1914 
   Size:        209     258      82     498     724     143

  Fragments arranged by size: 

                 724     498     258     209     143      82
 
AflIII A'CryG_T

Cuts at:      0     911    1673    1914 
   Size:        911     762     241

  Fragments arranged by size: 

                 911     762     241
 
AgeI A'CCGG_T

Cuts at:      0      81    1914 
   Size:         81    1833
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     733    1471    1914 
   Size:        733     738     443

  Fragments arranged by size: 

                 738     733     443
 
AlwNI CAG_nnn'CTG

Cuts at:      0     264    1233    1914 
   Size:        264     969     681

  Fragments arranged by size: 

                 969     681     264
 
ApaI G_GGCC'C

Cuts at:      0     811     842    1914 
   Size:        811      31    1072

  Fragments arranged by size: 

                1072     811      31
 
ApoI r'AATT_y

Cuts at:      0    1309    1494    1914 
   Size:       1309     185     420

  Fragments arranged by size: 

                1309     420     185
 
AvaI C'yCGr_G

Cuts at:      0     842    1087    1162    1691    1914 
   Size:        842     245      75     529     223

  Fragments arranged by size: 

                 842     529     245     223      75
 
BanI G'GyrC_C

Cuts at:      0    1348    1588    1696    1914 
   Size:       1348     240     108     218

  Fragments arranged by size: 

                1348     240     218     108
 
BanII G_rGCy'C

Cuts at:      0     811     842     851    1914 
   Size:        811      31       9    1063

  Fragments arranged by size: 

                1063     811      31       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      78     243    1691    1914 
   Size:         78     165    1448     223

  Fragments arranged by size: 

                1448     223     165      78
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1281    1315    1914 
   Size:       1281      34     599

  Fragments arranged by size: 

                1281     599      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     457    1914 
   Size:        457    1457
 
BclI T'GATC_A

Cuts at:      0     395    1914 
   Size:        395    1519
 
BglI GCCn_nnn'nGGC

Cuts at:      0     893    1099    1165    1914 
   Size:        893     206      66     749

  Fragments arranged by size: 

                 893     749     206      66
 
BlpI GC'TnA_GC

Cuts at:      0      74    1606    1914 
   Size:         74    1532     308

  Fragments arranged by size: 

                1532     308      74
 
Bme1580I G_kGCm'C

Cuts at:      0     811     842    1591    1791    1914 
   Size:        811      31     749     200     123

  Fragments arranged by size: 

                 811     749     200     123      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      15     413    1914 
   Size:         15     398    1501

  Fragments arranged by size: 

                1501     398      15
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     774    1056    1914 
   Size:        774     282     858

  Fragments arranged by size: 

                 858     774     282
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     954    1213    1531    1729    1876    1914 
   Size:        286     668     259     318     198     147      38

  Fragments arranged by size: 

                 668     318     286     259     198     147      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     266    1550    1914 
   Size:        266    1284     364

  Fragments arranged by size: 

                1284     364     266
 
BsaAI yAC'GTr

Cuts at:      0     912    1676    1914 
   Size:        912     764     238

  Fragments arranged by size: 

                 912     764     238
 
BsaBI GATnn'nnATC

Cuts at:      0     352     787    1914 
   Size:        352     435    1127

  Fragments arranged by size: 

                1127     435     352
 
BsaHI Gr'CG_yC

Cuts at:      0    1475    1614    1688    1914 
   Size:       1475     139      74     226

  Fragments arranged by size: 

                1475     226     139      74
 
BsaWI w'CCGG_w

Cuts at:      0      81     713    1914 
   Size:         81     632    1201

  Fragments arranged by size: 

                1201     632      81
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     517     547     668     698    1400    1430    1914 
   Size:        517      30     121      30     702      30     484

  Fragments arranged by size: 

                 702     517     484     121      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     747    1568    1864    1914 
   Size:        747     821     296      50

  Fragments arranged by size: 

                 821     747     296      50
 
BseYI C'CCAG_C

Cuts at:      0     459     803    1713    1914 
   Size:        459     344     910     201

  Fragments arranged by size: 

                 910     459     344     201
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     656    1110    1438    1914 
   Size:        656     454     328     476

  Fragments arranged by size: 

                 656     476     454     328
 
BsiEI CG_ry'CG

Cuts at:      0      35      82    1914 
   Size:         35      47    1832

  Fragments arranged by size: 

                1832      47      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     851    1244    1914 
   Size:        851     393     670

  Fragments arranged by size: 

                 851     670     393
 
BsmI GAATG_Cn'

Cuts at:      0    1137    1914 
   Size:       1137     777
 
Bsp1286I G_dGCh'C

Cuts at:      0     811     842     851    1244    1591    1791    1914 
   Size:        811      31       9     393     347     200     123

  Fragments arranged by size: 

                 811     393     347     200     123      31       9
 
BspEI T'CCGG_A

Cuts at:      0     713    1914 
   Size:        713    1201
 
BspHI T'CATG_A

Cuts at:      0     734    1661    1914 
   Size:        734     927     253

  Fragments arranged by size: 

                 927     734     253
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1336    1914 
   Size:       1336     578
 
BsrDI GCAATG_nn'

Cuts at:      0    1299    1914 
   Size:       1299     615
 
BsrFI r'CCGG_y

Cuts at:      0      81    1093    1610    1914 
   Size:         81    1012     517     304

  Fragments arranged by size: 

                1012     517     304      81
 
BtgI C'CryG_G

Cuts at:      0     621     863     888    1352    1877    1914 
   Size:        621     242      25     464     525      37

  Fragments arranged by size: 

                 621     525     464     242      37      25
 
BtsI GCAGTG_nn'

Cuts at:      0     391    1914 
   Size:        391    1523
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     654     689    1914 
   Size:        654      35    1225

  Fragments arranged by size: 

                1225     654      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1404    1914 
   Size:       1404     510
 
EaeI y'GGCC_r

Cuts at:      0      32     866    1832    1914 
   Size:         32     834     966      82

  Fragments arranged by size: 

                 966     834      82      32
 
EagI C'GGCC_G

Cuts at:      0      32    1914 
   Size:         32    1882
 
EarI CTCTTCn'nnn_

Cuts at:      0      52    1047    1545    1856    1914 
   Size:         52     995     498     311      58

  Fragments arranged by size: 

                 995     498     311      58      52
 
EcoICRI GAG'CTC

Cuts at:      0     849    1914 
   Size:        849    1065
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     286     467     549     954    1047    1213    1531 
   Size:        209      77     181      82     405      93     166     318

Cuts at:   1531    1729    1771    1876    1914 
   Size:        198      42     105      38

  Fragments arranged by size: 

                 405     318     209     198     181     166     105      93
                  82      77      42      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     223    1914 
   Size:        223    1691
 
EcoO109I rG'GnC_Cy

Cuts at:      0     626     807     808     839    1335    1422    1914 
   Size:        626     181       1      31     496      87     492

  Fragments arranged by size: 

                 626     496     492     181      87      31       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     198    1659    1914 
   Size:        198    1461     255

  Fragments arranged by size: 

                1461     255     198
 
HaeII r_GCGC'y

Cuts at:      0     800    1733    1914 
   Size:        800     933     181

  Fragments arranged by size: 

                 933     800     181
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     498     517     530     549    1599    1615    1631    1647 
   Size:        498      19      13      19    1050      16      16      16

Cuts at:   1647    1807    1839    1914 
   Size:        160      32      75

  Fragments arranged by size: 

                1050     498     160      75      32      19      19      16
                  16      16      13
 
HincII GTy'rAC

Cuts at:      0    1539    1914 
   Size:       1539     375
 
HindIII A'AGCT_T

Cuts at:      0     175    1914 
   Size:        175    1739
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     560     660    1914 
   Size:        560     100    1254

  Fragments arranged by size: 

                1254     560     100
 
MscI TGG'CCA

Cuts at:      0    1834    1914 
   Size:       1834      80
 
MslI CAynn'nnrTG

Cuts at:      0     358     715     910    1570    1914 
   Size:        358     357     195     660     344

  Fragments arranged by size: 

                 660     358     357     344     195
 
MspA1I CmG'CkG

Cuts at:      0     264     780     803     890    1156    1171    1914 
   Size:        264     516      23      87     266      15     743

  Fragments arranged by size: 

                 743     516     266     264      87      23      15
 
NaeI GCC'GGC

Cuts at:      0    1612    1914 
   Size:       1612     302
 
NcoI C'CATG_G

Cuts at:      0     863    1352    1914 
   Size:        863     489     562

  Fragments arranged by size: 

                 863     562     489
 
NdeI CA'TA_TG

Cuts at:      0     770    1914 
   Size:        770    1144
 
NgoMIV G'CCGG_C

Cuts at:      0    1610    1914 
   Size:       1610     304
 
NotI GC'GGCC_GC

Cuts at:      0      32    1914 
   Size:         32    1882
 
NspI r_CATG'y

Cuts at:      0     978    1227    1519    1914 
   Size:        978     249     292     395

  Fragments arranged by size: 

                 978     395     292     249
 
PasI CC'CwG_GG

Cuts at:      0     278    1914 
   Size:        278    1636
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     977    1404    1444    1914 
   Size:        977     427      40     470

  Fragments arranged by size: 

                 977     470     427      40
 
PmlI CAC'GTG

Cuts at:      0    1676    1914 
   Size:       1676     238
 
PpuMI rG'GwC_Cy

Cuts at:      0     626    1335    1422    1914 
   Size:        626     709      87     492

  Fragments arranged by size: 

                 709     626     492      87
 
PspOMI G'GGCC_C

Cuts at:      0     807     838    1914 
   Size:        807      31    1076

  Fragments arranged by size: 

                1076     807      31
 
PstI C_TGCA'G

Cuts at:      0     860    1155    1914 
   Size:        860     295     759

  Fragments arranged by size: 

                 860     759     295
 
PvuII CAG'CTG

Cuts at:      0     264     780     803    1156    1171    1914 
   Size:        264     516      23     353      15     743

  Fragments arranged by size: 

                 743     516     353     264      23      15
 
SacI G_AGCT'C

Cuts at:      0     851    1914 
   Size:        851    1063
 
SacII CC_GC'GG

Cuts at:      0     891    1914 
   Size:        891    1023
 
SanDI GG'GwC_CC

Cuts at:      0     626    1914 
   Size:        626    1288
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1047    1856    1914 
   Size:       1047     809      58

  Fragments arranged by size: 

                1047     809      58
 
SexAI A'CCwGG_T

Cuts at:      0    1802    1914 
   Size:       1802     112
 
SfcI C'TryA_G

Cuts at:      0     792     856    1151    1914 
   Size:        792      64     295     763

  Fragments arranged by size: 

                 792     763     295      64
 
SmaI CCC'GGG

Cuts at:      0     844    1914 
   Size:        844    1070
 
StuI AGG'CCT

Cuts at:      0     754    1914 
   Size:        754    1160
 
StyI C'CwwG_G

Cuts at:      0     698     863    1049    1352    1618    1634    1914 
   Size:        698     165     186     303     266      16     280

  Fragments arranged by size: 

                 698     303     280     266     186     165      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     329    1914 
   Size:        329    1585
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     618     650    1400    1432    1914 
   Size:        618      32     750      32     482

  Fragments arranged by size: 

                 750     618     482      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1273    1914 
   Size:       1273     641
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285     492    1359    1449    1914 
   Size:        285     207     867      90     465

  Fragments arranged by size: 

                 867     465     285     207      90
 
XmaI C'CCGG_G

Cuts at:      0     842    1914 
   Size:        842    1072
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI     BcgI    BciVI     BclI     BglI
     BlpI Bme1580I     BmrI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI     BtgI
     BtsI    CspCI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NotI
     NspI     PasI    PflMI     PmlI    PpuMI   PspOMI     PstI    PvuII
     SacI    SacII    SanDI     SapI    SexAI     SfcI     SmaI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI    BfrBI    BglII    BmgBI     BmtI   Bpu10I    BpuEI
    BsiWI    BsmBI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
     DrdI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NheI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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