################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BanI BbeI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FseI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MluI MroI MslI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AocI 8 CCTCAGG CC^T[ACGT]AGG AspHI 142 GAGCTC G[AT]GC[AT]^C AvaII 1207 GGACC G^G[AT]CC AviII 1181 TGCGCA TGC^GCA BalI 489 TGGCCA TGG^CCA BamHI 220 GGATCC G^GATCC BbrPI 610 CACGTG CAC^GTG BcnI 773 CCGGG CC^[GC]GG BglII 65 AGATCT A^GATCT BsaAI 610 CACGTG [CT]AC^GT[GA] BsiHKAI 142 GAGCTC G[AT]GC[AT]^C BspHI 1223 TCATGA T^CATGA BspMI 400 GCAGGT ACCTGC(4/8) BsrBI 973 GAGCGG CCGCTC(-3/-3) BstBI 959 TTCGAA TT^CGAA BstEII 795 GGTGACC G^GT[ACGT]ACC BstPI 795 GGTGACC G^GT[ACGT]ACC Bsu36I 8 CCTCAGG CC^T[ACGT]AGG DraI 1231 TTTAAA TTT^AAA EaeI 489 TGGCCA [CT]^GGCC[GA] Eco81I 8 CCTCAGG CC^T[ACGT]AGG EcoNI 679 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 795 GGTGACC G^GT[ACGT]ACC EcoT22I 350 ATGCAT ATGCA^T FauI 975 GCGGG CCCGC(4/6) FspI 1181 TGCGCA TGC^GCA HapII 773 CCGG C^CGG HincII 178 GTTGAC GT[CT]^[GA]AC HindII 178 GTTGAC GT[CT]^[GA]AC HpaII 773 CCGG C^CGG MfeI 437 CAATTG C^AATTG MluNI 489 TGGCCA TGG^CCA MscI 489 TGGCCA TGG^CCA MspI 773 CCGG C^CGG MunI 437 CAATTG C^AATTG NciI 773 CCGGG CC^[GC]GG NcoI 876 CCATGG C^CATGG NsiI 350 ATGCAT ATGCA^T NspV 959 TTCGAA TT^CGAA PflMI 436 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 610 CACGTG CAC^GTG PmlI 610 CACGTG CAC^GTG PstI 1339 CTGCAG CTGCA^G RcaI 1223 TCATGA T^CATGA SacI 142 GAGCTC GAGCT^C SfuI 959 TTCGAA TT^CGAA SmlI 1022 CTCAAG C^T[CT][GA]AG SspI 658 AATATT AAT^ATT Van91I 436 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BanII 142 GAGCTC G[GA]GC[CT]^C 819 GAGCCC BclI 758 TGATCA T^GATCA 1250 TGATCA BpmI 647 CTGGAG CTGGAG(16/14) 802 CTGGAG BsrDI 386 CATTGC GCAATG(2/0) 1185 CATTGC CfoI 390 GCGC GCG^C 1182 GCGC DraII 540 AGGGCCT [GA]G^G[ACGT]CC[CT] 1026 AGGCCCT DrdI 22 GACTTACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 312 GACAATGGAGTC EarI 789 GAAGAG CTCTTC(1/4) 1424 GAAGAG EcoO109I 540 AGGGCCT [GA]G^G[ACGT]CC[CT] 1026 AGGCCCT FbaI 758 TGATCA T^GATCA 1250 TGATCA HhaI 390 GCGC GCG^C 1182 GCGC HinP1I 390 GCGC G^CGC 1182 GCGC Ksp632I 789 GAAGAG CTCTTC(1/4) 1424 GAAGAG MaeII 611 ACGT A^CGT 1247 ACGT NlaIV 220 GGATCC GG[ACGT]^[ACGT]CC 1093 GGGGCC PleI 319 GAGTC GAGTC(4/5) 1016 GAGTC SfcI 680 CTACAG C^T[GA][CT]AG 1339 CTGCAG TaqI 287 TCGA T^CGA 960 TCGA TthHB8I 287 TCGA T^CGA 960 TCGA XcmI 920 CCAGGATTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1386 CCAGTGCCCCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BmyI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII