################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII CfoI ClaI CpoI DraI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MluNI MroI MscI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 507 CGCG CG^CG AfaI 10 GTAC GT^AC AflIII 506 ACGCGT A^C[GA][CT]GT Alw44I 1068 GTGCAC G^TGCAC AocI 672 CCTGAGG CC^T[ACGT]AGG ApaI 43 GGGCCC GGGCC^C ApaLI 1068 GTGCAC G^TGCAC Asp700I 232 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 138 GGATCC G^GATCC BanI 65 GGCACC G^G[CT][GA]CC BbsI 753 GAAGAC GAAGAC(2/6) BfaI 604 CTAG C^TAG BpuAI 753 GAAGAC GAAGAC(2/6) BsaAI 244 CACGTA [CT]AC^GT[GA] BsaI 163 GGTCTC GGTCTC(1/5) BsmAI 164 GTCTC GTCTC(1/5) BspMI 852 ACCTGC ACCTGC(4/8) BsrBI 736 GAGCGG CCGCTC(-3/-3) BsrFI 686 ACCGGC [GA]^CCGG[CT] BstAPI 654 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 507 CGCG CG^CG BstXI 153 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 672 CCTGAGG CC^T[ACGT]AGG Cfr10I 686 ACCGGC [GA]^CCGG[CT] DrdI 982 GACAAACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1080 GAAGAG CTCTTC(1/4) Eco81I 672 CCTGAGG CC^T[ACGT]AGG EcoT14I 153 CCAAGG C^C[AT][AT]GG FauI 55 CCCGC CCCGC(4/6) HgaI 508 GCGTC GACGC(5/10) HincII 547 GTTGAC GT[CT]^[GA]AC HindII 547 GTTGAC GT[CT]^[GA]AC HindIII 172 AAGCTT A^AGCTT Ksp632I 1080 GAAGAG CTCTTC(1/4) MaeI 604 CTAG C^TAG MluI 506 ACGCGT A^CGCGT MvnI 507 CGCG CG^CG NheI 603 GCTAGC G^CTAGC PpuMI 377 GGGACCT [GA]G^G[AT]CC[CT] PvuII 106 CAGCTG CAG^CTG RsaI 10 GTAC GT^AC SapI 1080 GAAGAGC GCTCTTC(1/4) SmaI 46 CCCGGG CCC^GGG StyI 153 CCAAGG C^C[AT][AT]GG TfiI 213 GAATC G^A[AT]TC Tsp45I 442 GTGAC ^GT[GC]AC XmaCI 46 CCCGGG C^CCGGG XmaI 46 CCCGGG C^CCGGG XmnI 232 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 196 GAATTT [GA]^AATT[CT] 812 AAATTC ApoI 196 GAATTT [GA]^AATT[CT] 812 AAATTC AvaI 46 CCCGGG C^[CT]CG[GA]G 1013 CCCGAG AvaII 378 GGACC G^G[AT]CC 1050 GGACC BseRI 646 CTCCTC GAGGAG(10/8) 76 GAGGAG BsgI 133 GTGCAG GTGCAG(16/14) 1006 GTGCAG BsoBI 46 CCCGGG C^[CT]CG[GA]G 1013 CCCGAG BsrI 476 CCAGT ACTGG(1/-1) 565 CCAGT BssSI 226 CTCGTG CACGAG(-5/-1) 299 CTCGTG BstF5I 1119 CATCC GGATG(2/0) 1074 GGATG BstYI 138 GGATCC [GA]^GATC[CT] 558 GGATCT DraII 377 GGGACCT [GA]G^G[ACGT]CC[CT] 938 AGGCCCT DraIII 205 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 655 CACCCTGTG EcoO109I 377 GGGACCT [GA]G^G[ACGT]CC[CT] 938 AGGCCCT FokI 1119 CATCC GGATG(9/13) 1074 GGATG MaeIII 388 GTTAC ^GT[ACGT]AC 442 GTGAC MfeI 350 CAATTG C^AATTG 401 CAATTG MflI 138 GGATCC [GA]^GATC[CT] 558 GGATCT MspA1I 56 CCGCTG C[AC]G^C[GT]G 106 CAGCTG MunI 350 CAATTG C^AATTG 401 CAATTG SfcI 22 CTACAG C^T[GA][CT]AG 382 CTACAG XhoII 138 GGATCC [GA]^GATC[CT] 558 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI SacI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp509I TspRI