Restriction map for pF1KB3778
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AflIII      AgeI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaJI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII           5  CGCG                 CG^CG
AcyI          534  GACGCC               G[GA]^CG[CT]C
AhdI          534  GACGCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI           90  GGGCCC               GGGCC^C
AspEI         534  GACGCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          366  GGCACC               G^G[CT][GA]CC
BanII          90  GGGCCC               G[GA]GC[CT]^C
BmyI           90  GGGCCC               G[AGT]GC[ACT]^C
BsaHI         534  GACGCC               G[GA]^CG[CT]C
BsaWI         633  ACCGGA               [AT]^CCGG[AT]
BseRI         355  GAGGAG               GAGGAG(10/8)
Bsp1286I       90  GGGCCC               G[AGT]GC[ACT]^C
BsrI          544  CCAGT                ACTGG(1/-1)
BssKI         375  CCAGG                ^CC[ACGT]GG
BstBI         444  TTCGAA               TT^CGAA
BstNI         375  CCAGG                CC^[AT]GG
BstUI           5  CGCG                 CG^CG
CfoI          267  GCGC                 GCG^C
DraII          90  GGGCCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      534  GACGCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I       90  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         156  GAATTC               G^AATTC
EcoRII        375  CCAGG                ^CC[AT]GG
FauI          642  CCCGC                CCCGC(4/6)
HgaI          534  GACGC                GACGC(5/10)
HhaI          267  GCGC                 GCG^C
Hin1I         534  GACGCC               G[GA]^CG[CT]C
HinP1I        267  GCGC                 G^CGC
MseI          470  TTAA                 T^TAA
MslI          256  CATAAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          375  CCAGG                CC^[AT]GG
MvnI            5  CGCG                 CG^CG
NlaIII        321  CATG                 CATG^
NspV          444  TTCGAA               TT^CGAA
ScrFI         375  CCAGG                CC^[ACGT]GG
SfcI          527  CTACAG               C^T[GA][CT]AG
SfuI          444  TTCGAA               TT^CGAA
Tru9I         470  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          107  AAATTC               [GA]^AATT[CT]
              156  GAATTC              
ApoI          107  AAATTC               [GA]^AATT[CT]
              156  GAATTC              
Asp700I        80  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              152  GAAAGAATTC          
AvaI          122  CCCGAG               C^[CT]CG[GA]G
              676  CCCGAG              
BbsI          184  GAAGAC               GAAGAC(2/6)
              198  GAAGAC              
BbvI          295  GCAGC                GCAGC(8/12)
              392  GCAGC               
BpuAI         184  GAAGAC               GAAGAC(2/6)
              198  GAAGAC              
BsoBI         122  CCCGAG               C^[CT]CG[GA]G
              676  CCCGAG              
Cac8I         279  GCAGGC               GC[ACGT]^[ACGT]GC
              641  GCCCGC              
Cfr13I         90  GGGCC                G^G[ACGT]CC
               91  GGCCC               
EarI           80  GAAGAG               CTCTTC(1/4)
              352  GAAGAG              
HaeIII          8  GGCC                 GG^CC
               91  GGCC                
HapII         389  CCGG                 C^CGG
              634  CCGG                
HpaII         389  CCGG                 C^CGG
              634  CCGG                
Ksp632I        80  GAAGAG               CTCTTC(1/4)
              352  GAAGAG              
MaeIII        431  GTGAC                ^GT[ACGT]AC
              532  GTGAC               
MspI          389  CCGG                 C^CGG
              634  CCGG                
MwoI          286  GCATCTAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              580  GCTTCAAAGGC         
PleI          178  GACTC                GAGTC(4/5)
              603  GACTC               
Sau96I         90  GGGCC                G^G[ACGT]CC
               91  GGCCC               
TaqI          111  TCGA                 T^CGA
              445  TCGA                
TseI          295  GCAGC                G^C[AT]GC
              392  GCAGC               
Tsp45I        431  GTGAC                ^GT[GC]AC
              532  GTGAC               
Tsp509I       108  AATT                 ^AATT
              157  AATT                
TthHB8I       111  TCGA                 T^CGA
              445  TCGA                
XmnI           80  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              152  GAAAGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AciI        AluI        BsiYI       BslI        BstF5I      
DdeI        DpnII       Fnu4HI      FokI        HinfI       
ItaI        MboI        MboII       MnlI        NdeII       
NlaIV       Sau3AI      SfaNI       TfiI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com