Restriction map for pF1KB6376
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          370  GAATTC               [GA]^AATT[CT]
AfaI          564  GTAC                 GT^AC
AlwI          756  GGATC                GGATC(4/5)
ApoI          370  GAATTC               [GA]^AATT[CT]
Asp700I       204  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          71  GTGCTC               G[AT]GC[AT]^C
BanI          868  GGTGCC               G^G[CT][GA]CC
BcgI          609  GCAGAGTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         594  GTATCC               GTATCC(6/5)
BclI          890  TGATCA               T^GATCA
BfaI          478  CTAG                 C^TAG
BsaI          703  GAGACC               GGTCTC(1/5)
BseRI          55  GAGGAG               GAGGAG(10/8)
BsiHKAI        71  GTGCTC               G[AT]GC[AT]^C
BsrDI         268  GCAATG               GCAATG(2/0)
BstXI         461  CCAAGGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         756  GGATCT               [GA]^GATC[CT]
Cac8I         801  GCTGGC               GC[ACGT]^[ACGT]GC
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoRI         370  GAATTC               G^AATTC
FbaI          890  TGATCA               T^GATCA
HgaI          668  GCGTC                GACGC(5/10)
HphI          877  GGTGA                GGTGA(8/7)
MaeI          478  CTAG                 C^TAG
MflI          756  GGATCT               [GA]^GATC[CT]
MspA1I          8  CCGCTG               C[AC]G^C[GT]G
NcoI          467  CCATGG               C^CATGG
NspI          440  GCATGT               [GA]CATG^[CT]
PflMI         297  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          612  GAGTC                GAGTC(4/5)
PstI          332  CTGCAG               CTGCA^G
RsaI          564  GTAC                 GT^AC
SapI          451  GAAGAGC              GCTCTTC(1/4)
TfiI          622  GAATC                G^A[AT]TC
Van91I        297  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         756  GGATCT               [GA]^GATC[CT]
XmnI          204  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwNI         326  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              883  CAGTGTCTG           
ApaI          145  GGGCCC               GGGCC^C
              858  GGGCCC              
BanII         145  GGGCCC               G[GA]GC[CT]^C
              858  GGGCCC              
BbvCI          10  GCTGAGG              CCTCAGC(-5/-2)
              283  GCTGAGG             
BsiYI         297  CCACCTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              467  CCATGGAGGGG         
BslI          297  CCACCTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              467  CCATGGAGGGG         
BsmAI         167  GAGAC                GTCTC(1/5)
              703  GAGAC               
BsrI          234  ACTGG                ACTGG(1/-1)
              872  CCAGT               
DraII         144  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              145  GGGCCCC             
DsaI          125  CCACGG               C^C[GA][CT]GG
              467  CCATGG              
EcoO109I      144  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              145  GGGCCCC             
HinfI         612  GAGTC                G^A[ACGT]TC
              622  GAATC               
SexAI         299  ACCTGGT              A^CC[AT]GGT
              864  ACCAGGT             
SfcI           21  CTGTAG               C^T[GA][CT]AG
              332  CTGCAG              
Tsp509I        50  AATT                 ^AATT
              371  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        BbvI        BmyI        BpmI        
BsaJI       Bsp1286I    BssKI       BstF5I      BstNI       
Cfr13I      DdeI        DpnII       DraIII      EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
ItaI        Ksp632I     MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        TspRI       TthHB8I     
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