Restriction map for pF1KB6376
 (Linear) (Six-base) MAPSORT of: KIBB6376.seq  Check: 7323  from: 1  to: 897



KIBB6376.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     595     897 
   Size:        412     183     302

  Fragments arranged by size: 

                 412     302     183
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     532     564     897 
   Size:        532      32     333

  Fragments arranged by size: 

                 532     333      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     331     888     897 
   Size:        331     557       9

  Fragments arranged by size: 

                 557     331       9
 
ApaI G_GGCC'C

Cuts at:      0     149     862     897 
   Size:        149     713      35

  Fragments arranged by size: 

                 713     149      35
 
ApoI r'AATT_y

Cuts at:      0     370     897 
   Size:        370     527
 
BanI G'GyrC_C

Cuts at:      0     868     897 
   Size:        868      29
 
BanII G_rGCy'C

Cuts at:      0     149     862     897 
   Size:        149     713      35

  Fragments arranged by size: 

                 713     149      35
 
BbvCI CC'TCA_GC

Cuts at:      0      11     284     897 
   Size:         11     273     613

  Fragments arranged by size: 

                 613     273      11
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     598     632     897 
   Size:        598      34     265

  Fragments arranged by size: 

                 598     265      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     605     897 
   Size:        605     292
 
BclI T'GATC_A

Cuts at:      0     890     897 
   Size:        890       7
 
Bme1580I G_kGCm'C

Cuts at:      0     149     862     897 
   Size:        149     713      35

  Fragments arranged by size: 

                 713     149      35
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     617     827     897 
   Size:        420     197     210      70

  Fragments arranged by size: 

                 420     210     197      70
 
Bpu10I CC'TnA_GC

Cuts at:      0      11     284     897 
   Size:         11     273     613

  Fragments arranged by size: 

                 613     273      11
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     697     897 
   Size:        697     200
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     532     562     897 
   Size:        532      30     335

  Fragments arranged by size: 

                 532     335      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     897 
   Size:         70     827
 
BseYI C'CCAG_C

Cuts at:      0     105     897 
   Size:        105     792
 
BsiHKAI G_wGCw'C

Cuts at:      0      75     897 
   Size:         75     822
 
Bsp1286I G_dGCh'C

Cuts at:      0      75     149     862     897 
   Size:         75      74     713      35

  Fragments arranged by size: 

                 713      75      74      35
 
BsrDI GCAATG_nn'

Cuts at:      0     275     897 
   Size:        275     622
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     468     897 
   Size:        468     429
 
BstYI r'GATC_y

Cuts at:      0     756     897 
   Size:        756     141
 
BtgI C'CryG_G

Cuts at:      0     125     467     897 
   Size:        125     342     430

  Fragments arranged by size: 

                 430     342     125
 
BtsI GCAGTG_nn'

Cuts at:      0     170     248     354     897 
   Size:        170      78     106     543

  Fragments arranged by size: 

                 543     170     106      78
 
DraIII CAC_nnn'GTG

Cuts at:      0     303     400     868     897 
   Size:        303      97     468      29

  Fragments arranged by size: 

                 468     303      97      29
 
EaeI y'GGCC_r

Cuts at:      0       2     897 
   Size:          2     895
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     193     446     897 
   Size:        148      45     253     451

  Fragments arranged by size: 

                 451     253     148      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     420     595     617     827     897 
   Size:        412       8     175      22     210      70

  Fragments arranged by size: 

                 412     210     175      70      22       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     145     146     897 
   Size:        145       1     751

  Fragments arranged by size: 

                 751     145       1
 
EcoRI G'AATT_C

Cuts at:      0     370     897 
   Size:        370     527
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     897 
   Size:        343      32     522

  Fragments arranged by size: 

                 522     343      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     897 
   Size:        425     472
 
MspA1I CmG'CkG

Cuts at:      0      10     897 
   Size:         10     887
 
NcoI C'CATG_G

Cuts at:      0     467     897 
   Size:        467     430
 
NspI r_CATG'y

Cuts at:      0     444     897 
   Size:        444     453
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     303     897 
   Size:        303     594
 
PfoI T'CCnGG_A

Cuts at:      0     212     897 
   Size:        212     685
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     532     564     897 
   Size:        532      32     333

  Fragments arranged by size: 

                 532     333      32
 
PspOMI G'GGCC_C

Cuts at:      0     145     858     897 
   Size:        145     713      39

  Fragments arranged by size: 

                 713     145      39
 
PstI C_TGCA'G

Cuts at:      0     336     897 
   Size:        336     561
 
SapI GCTCTTCn'nnn_

Cuts at:      0     446     897 
   Size:        446     451
 
SexAI A'CCwGG_T

Cuts at:      0     299     864     897 
   Size:        299     565      33

  Fragments arranged by size: 

                 565     299      33
 
SfcI C'TryA_G

Cuts at:      0      21     332     897 
   Size:         21     311     565

  Fragments arranged by size: 

                 565     311      21
 
StyI C'CwwG_G

Cuts at:      0     461     467     551     897 
   Size:        461       6      84     346

  Fragments arranged by size: 

                 461     346      84       6
 
XmnI GAAnn'nnTTC

Cuts at:      0     208     897 
   Size:        208     689
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApaI     ApoI     BanI    BanII    BbvCI
     BcgI    BciVI     BclI Bme1580I     BpmI   Bpu10I     BsaI    BsaXI
    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI    BstXI    BstYI     BtgI
     BtsI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I
     MmeI   MspA1I     NcoI     NspI    PflMI     PfoI     PpiI   PspOMI
     PstI     SapI    SexAI     SfcI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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