Restriction map for pF1KB3606
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaI        BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmI        BsoBI       Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     KspI        
MaeI        MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StyI        SwaI        
TfiI        TseI        Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AlwI          257  GGATC                GGATC(4/5)
AlwNI          32  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          288  GGGCCC               GGGCC^C
BanI           67  GGCGCC               G^G[CT][GA]CC
BanII         288  GGGCCC               G[GA]GC[CT]^C
BbeI           67  GGCGCC               GGCGC^C
BcnI          269  CCCGG                CC^[GC]GG
BsaAI         109  TACGTG               [CT]AC^GT[GA]
BsaWI           7  TCCGGT               [AT]^CCGG[AT]
BsgI          182  GTGCAG               GTGCAG(16/14)
BsmAI         223  GTCTC                GTCTC(1/5)
BsmBI         222  CGTCTC               CGTCTC(1/5)
BspDI         394  ATCGAT               AT^CGAT
BsrDI         369  CATTGC               GCAATG(2/0)
BsrI          486  CCAGT                ACTGG(1/-1)
BssHII         16  GCGCGC               G^CGCGC
BstXI          50  CCACAGCGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          394  ATCGAT               AT^CGAT
DsaI          452  CCGTGG               C^C[GA][CT]GG
FauI          517  CCCGC                CCCGC(4/6)
Fnu4HI        468  GCGGC                GC^[ACGT]GC
HaeII          67  GGCGCC               [GA]GCGC^[CT]
HinfI         232  GACTC                G^A[ACGT]TC
ItaI          468  GCGGC                GC^[ACGT]GC
KasI           67  GGCGCC               G^GCGCC
MwoI          197  GCAAGCTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI           67  GGCGCC               GG^CGCC
NciI          269  CCCGG                CC^[GC]GG
NlaIII        502  CATG                 CATG^
PleI          232  GACTC                GAGTC(4/5)
PshAI         326  GACGCTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI          20  GCATC                GCATC(5/9)
SfcI          375  CTACAG               C^T[GA][CT]AG
SfoI           67  GGCGCC               GGC^GCC
SspI          575  AATATT               AAT^ATT
StuI          479  AGGCCT               AGG^CCT
TaqI          395  TCGA                 T^CGA
TthHB8I       395  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI           67  GGCGCC               G[GA]^CG[CT]C
              220  GGCGTC              
AfaI          108  GTAC                 GT^AC
              489  GTAC                
AluI           86  AGCT                 AG^CT
              200  AGCT                
AvaII         114  GGTCC                G^G[AT]CC
              474  GGTCC               
BmyI          150  GTGCCC               G[AGT]GC[ACT]^C
              288  GGGCCC              
BsaHI          67  GGCGCC               G[GA]^CG[CT]C
              220  GGCGTC              
BsaJI         268  CCCCGG               C^C[ACGT][ACGT]GG
              452  CCGTGG              
BsmFI         444  GGGAC                GGGAC(10/14)
              515  GTCCC               
Bsp1286I      150  GTGCCC               G[AGT]GC[ACT]^C
              288  GGGCCC              
DdeI          236  CTCAG                C^T[ACGT]AG
              335  CTGAG               
DraII         288  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              380  GGGGCCT             
EcoO109I      288  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              380  GGGGCCT             
HapII           8  CCGG                 C^CGG
              270  CCGG                
Hin1I          67  GGCGCC               G[GA]^CG[CT]C
              220  GGCGTC              
HpaII           8  CCGG                 C^CGG
              270  CCGG                
HphI           81  GGTGA                GGTGA(8/7)
              242  TCACC               
MseI          428  TTAA                 T^TAA
              548  TTAA                
MslI           51  CACAGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              141  CACTTTTGTG          
MspA1I         53  CAGCGG               C[AC]G^C[GT]G
              518  CCGCTG              
MspI            8  CCGG                 C^CGG
              270  CCGG                
RsaI          108  GTAC                 GT^AC
              489  GTAC                
Tru9I         428  TTAA                 T^TAA
              548  TTAA                
Tsp45I        319  GTGAC                ^GT[GC]AC
              539  GTGAC               
TspRI         423  CACTG                CA[GC]TG[ACGT][ACGT]^
              537  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AccII       AciI        BsiYI       BslI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DpnII       EcoRII      FokI        HaeIII      
HgaI        HhaI        HinP1I      MaeII       MaeIII      
MboI        MnlI        MvaI        MvnI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       
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