Restriction map for
pF1KB6602
(Circular) (Six-base) MAPSORT of: pF1KB6602.seq Check: 4978 from: 1 to: 4403
pF1KB6602 4403 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1441 1441
Size: 4403
Acc65I G'GTAC_C
Cuts at: 1419 1419
Size: 4403
AclI AA'CG_TT
Cuts at: 4243 4243
Size: 4403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 1075 2228 2660 3407 3675 252
Size: 823 1153 432 747 268 980
Fragments arranged by size:
1153 980 823 747 432 268
AflIII A'CryG_T
Cuts at: 1755 2859 3836 1755
Size: 1104 977 2322
Fragments arranged by size:
2322 1104 977
AgeI A'CCGG_T
Cuts at: 146 2791 146
Size: 2645 1758
AhdI GACnn_n'nnGTC
Cuts at: 472 1012 472
Size: 540 3863
AleI CACnn'nnGTG
Cuts at: 946 946
Size: 4403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 577 1327 577
Size: 750 3653
AlwNI CAG_nnn'CTG
Cuts at: 223 482 1708 3275 223
Size: 259 1226 1567 1351
Fragments arranged by size:
1567 1351 1226 259
ApaI G_GGCC'C
Cuts at: 870 870
Size: 4403
ApaLI G'TGCA_C
Cuts at: 3173 3173
Size: 4403
ApoI r'AATT_y
Cuts at: 1407 3756 4012 1407
Size: 2349 256 1798
Fragments arranged by size:
2349 1798 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4403
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
AvaI C'yCGr_G
Cuts at: 1163 1423 1163
Size: 260 4143
BamHI G'GATC_C
Cuts at: 1127 1428 1127
Size: 301 4102
BanI G'GyrC_C
Cuts at: 168 181 1419 1833 2081 2116 168
Size: 13 1238 414 248 35 2455
Fragments arranged by size:
2455 1238 414 248 35 13
BanII G_rGCy'C
Cuts at: 160 232 522 662 870 1417 2447 160
Size: 72 290 140 208 547 1030 2116
Fragments arranged by size:
2116 1030 547 290 208 140 72
BbeI G_GCGC'C
Cuts at: 172 185 1837 2085 172
Size: 13 1652 248 2490
Fragments arranged by size:
2490 1652 248 13
BbvCI CC'TCA_GC
Cuts at: 968 968
Size: 4403
BciVI GTATCCnnnnn_n'
Cuts at: 2301 3062 4403 2301
Size: 761 1341 2301
Fragments arranged by size:
2301 1341 761
BclI T'GATC_A
Cuts at: 1923 1923
Size: 4403
BglII A'GATC_T
Cuts at: 524 1918 3955 524
Size: 1394 2037 972
Fragments arranged by size:
2037 1394 972
BlpI GC'TnA_GC
Cuts at: 1515 1515
Size: 4403
Bme1580I G_kGCm'C
Cuts at: 870 2028 2121 3177 870
Size: 1158 93 1056 2096
Fragments arranged by size:
2096 1158 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1615 1696 2031 4175 1615
Size: 81 335 2144 1843
Fragments arranged by size:
2144 1843 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 451 504 724 1246 430
Size: 21 53 220 522 3587
Fragments arranged by size:
3587 522 220 53 21
Bpu10I CC'TnA_GC
Cuts at: 968 968
Size: 4403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1162 1579 2458 2950 3248 709
Size: 453 417 879 492 298 1864
Fragments arranged by size:
1864 879 492 453 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 346 406 926 1363 4107 346
Size: 60 520 437 2744 642
Fragments arranged by size:
2744 642 520 437 60
BsaAI yAC'GTr
Cuts at: 1756 1756
Size: 4403
BsaBI GATnn'nnATC
Cuts at: 6 1917 3954 6
Size: 1911 2037 455
Fragments arranged by size:
2037 1911 455
BsaHI Gr'CG_yC
Cuts at: 169 182 1834 2082 2784 4280 169
Size: 13 1652 248 702 1496 292
Fragments arranged by size:
1652 1496 702 292 248 13
BsaWI w'CCGG_w
Cuts at: 146 422 1592 2113 2791 3065 3212 3623 146
Size: 276 1170 521 678 274 147 411 926
Fragments arranged by size:
1170 926 678 521 411 276 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1024 1054 1024
Size: 30 4373
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 417 745 916 949 417
Size: 328 171 33 3871
Fragments arranged by size:
3871 328 171 33
BseYI C'CCAG_C
Cuts at: 172 223 1828 2586 3163 3659 172
Size: 51 1605 758 577 496 916
Fragments arranged by size:
1605 916 758 577 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1120
Size: 4403
BsiEI CG_ry'CG
Cuts at: 104 190 375 1945 1991 3199 104
Size: 86 185 1570 46 1208 1308
Fragments arranged by size:
1570 1308 1208 185 86 46
BsiHKAI G_wGCw'C
Cuts at: 452 943 1417 2195 2385 3177 452
Size: 491 474 778 190 792 1678
Fragments arranged by size:
1678 792 778 491 474 190
BsmBI CGTCTCn'nnnn_
Cuts at: 979 999 979
Size: 20 4383
Bsp1286I G_dGCh'C
Cuts at: 160 232 452 522 662 870 943 1417 2028
Size: 72 220 70 140 208 73 474 611
Cuts at: 2028 2121 2195 2385 2447 3177 160
Size: 93 74 190 62 730 1386
Fragments arranged by size:
1386 730 611 474 220 208 190 140
93 74 73 72 70 62
BspEI T'CCGG_A
Cuts at: 3623 3623
Size: 4403
BspHI T'CATG_A
Cuts at: 743 3579 743
Size: 2836 1567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1453 1667 1969 2350 1453
Size: 214 302 381 3506
Fragments arranged by size:
3506 381 302 214
BsrBI CCG'CTC
Cuts at: 50 257 2695 2749 4233 4398 50
Size: 207 2438 54 1484 165 55
Fragments arranged by size:
2438 1484 207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2315 2315
Size: 4403
BsrFI r'CCGG_y
Cuts at: 146 189 2401 2582 2791 146
Size: 43 2212 181 209 1758
Fragments arranged by size:
2212 1758 209 181 43
BssSI C'ACGA_G
Cuts at: 2674 3032 2674
Size: 358 4045
BstAPI GCAn_nnn'nTGC
Cuts at: 1780 1780
Size: 4403
BstBI TT'CG_AA
Cuts at: 2764 2764
Size: 4403
BstEII G'GTnAC_C
Cuts at: 951 951
Size: 4403
BstXI CCAn_nnnn'nTGG
Cuts at: 852 852
Size: 4403
BstYI r'GATC_y
Cuts at: 1 242 524 1127 1428 1895 1918 2253 2499
Size: 241 282 603 301 467 23 335 246
Cuts at: 2499 3511 3597 3609 3955 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 603 467 449 346 335 301 282
246 241 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 514 1078 514
Size: 564 3839
BtgI C'CryG_G
Cuts at: 654 809 654
Size: 155 4248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2353 2534 2697 2353
Size: 181 163 4059
Fragments arranged by size:
4059 181 163
BtsI GCAGTG_nn'
Cuts at: 1646 1646
Size: 4403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 521 556 521
Size: 35 4368
DraI TTT'AAA
Cuts at: 1402 1402
Size: 4403
DrdI GACnn_nn'nnGTC
Cuts at: 601 2109 2967 601
Size: 1508 858 2037
Fragments arranged by size:
2037 1508 858
EaeI y'GGCC_r
Cuts at: 187 203 296 623 1988 2162 2553 2580 3925
Size: 16 93 327 1365 174 391 27 1345
Cuts at: 3925 187
Size: 665
Fragments arranged by size:
1365 1345 665 391 327 174 93 27
16
EagI C'GGCC_G
Cuts at: 187 1988 187
Size: 1801 2602
EarI CTCTTCn'nnn_
Cuts at: 745 755 2426 2636 745
Size: 10 1671 210 2512
Fragments arranged by size:
2512 1671 210 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2611 2921 3067 4213 2611
Size: 310 146 1146 2801
Fragments arranged by size:
2801 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1415 1415
Size: 4403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 430 451 504 724 1075 1246 2228 2660
Size: 178 21 53 220 351 171 982 432
Cuts at: 2660 3407 3675 252
Size: 747 268 980
Fragments arranged by size:
982 980 747 432 351 268 220 178
171 53 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1081 1081
Size: 4403
EcoO109I rG'GnC_Cy
Cuts at: 291 466 510 837 1200 1542 291
Size: 175 44 327 363 342 3152
Fragments arranged by size:
3152 363 342 327 175 44
EcoRI G'AATT_C
Cuts at: 1407 1407
Size: 4403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1080 2422 1080
Size: 1342 3061
FspI TGC'GCA
Cuts at: 2184 2184
Size: 4403
HaeII r_GCGC'y
Cuts at: 172 185 224 1182 1837 2085 3107 4086 172
Size: 13 39 958 655 248 1022 979 489
Fragments arranged by size:
1022 979 958 655 489 248 39 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 458 490 705 737 998 1024 1030 1056 2245
Size: 32 215 32 261 26 6 26 1189
Cuts at: 2245 2275 2277 2307 458
Size: 30 2 30 2554
Fragments arranged by size:
2554 1189 261 215 32 32 30 30
26 26 6 2
HincII GTy'rAC
Cuts at: 806 1442 806
Size: 636 3767
KasI G'GCGC_C
Cuts at: 168 181 1833 2081 168
Size: 13 1652 248 2490
Fragments arranged by size:
2490 1652 248 13
KpnI G_GTAC'C
Cuts at: 1423 1423
Size: 4403
MluI A'CGCG_T
Cuts at: 3836 3836
Size: 4403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 954 3074 3258 954
Size: 2120 184 2099
Fragments arranged by size:
2120 2099 184
MscI TGG'CCA
Cuts at: 298 625 2164 298
Size: 327 1539 2537
Fragments arranged by size:
2537 1539 327
MslI CAynn'nnrTG
Cuts at: 946 2392 2519 946
Size: 1446 127 2830
Fragments arranged by size:
2830 1446 127
MspA1I CmG'CkG
Cuts at: 702 1514 1828 2188 3201 3446 702
Size: 812 314 360 1013 245 1659
Fragments arranged by size:
1659 1013 812 360 314 245
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 4403
NarI GG'CG_CC
Cuts at: 169 182 1834 2082 169
Size: 13 1652 248 2490
Fragments arranged by size:
2490 1652 248 13
NcoI C'CATG_G
Cuts at: 654 654
Size: 4403
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 4403
NspI r_CATG'y
Cuts at: 1399 1456 1956 2863 1399
Size: 57 500 907 2939
Fragments arranged by size:
2939 907 500 57
PasI CC'CwG_GG
Cuts at: 153 153
Size: 4403
PciI A'CATG_T
Cuts at: 2859 2859
Size: 4403
PflMI CCAn_nnn'nTGG
Cuts at: 639 1023 639
Size: 384 4019
PfoI T'CCnGG_A
Cuts at: 238 443 238
Size: 205 4198
PmeI GTTT'AAAC
Cuts at: 1402 1402
Size: 4403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 4371
PpuMI rG'GwC_Cy
Cuts at: 466 510 837 466
Size: 44 327 4032
Fragments arranged by size:
4032 327 44
PspOMI G'GGCC_C
Cuts at: 866 866
Size: 4403
PstI C_TGCA'G
Cuts at: 566 701 1450 2135 566
Size: 135 749 685 2834
Fragments arranged by size:
2834 749 685 135
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4403
PvuII CAG'CTG
Cuts at: 702 1828 2188 702
Size: 1126 360 2917
Fragments arranged by size:
2917 1126 360
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 4403
SacI G_AGCT'C
Cuts at: 1417 1417
Size: 4403
SalI G'TCGA_C
Cuts at: 1440 1440
Size: 4403
SanDI GG'GwC_CC
Cuts at: 466 466
Size: 4403
SapI GCTCTTCn'nnn_
Cuts at: 745 2426 2636 745
Size: 1681 210 2512
Fragments arranged by size:
2512 1681 210
SbfI CC_TGCA'GG
Cuts at: 1450 1450
Size: 4403
ScaI AGT'ACT
Cuts at: 916 3920 916
Size: 3004 1399
SfcI C'TryA_G
Cuts at: 33 562 697 1105 1446 2131 3124 3315 33
Size: 529 135 408 341 685 993 191 1121
Fragments arranged by size:
1121 993 685 529 408 341 191 135
SfoI GGC'GCC
Cuts at: 170 183 1835 2083 170
Size: 13 1652 248 2490
Fragments arranged by size:
2490 1652 248 13
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
SmaI CCC'GGG
Cuts at: 1165 1425 1165
Size: 260 4143
SmlI C'TyrA_G
Cuts at: 724 1141 1558 2473 2965 3227 724
Size: 417 417 915 492 262 1900
Fragments arranged by size:
1900 915 492 417 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4403
SphI G_CATG'C
Cuts at: 1456 1956 1456
Size: 500 3903
StuI AGG'CCT
Cuts at: 776 776
Size: 4403
StyI C'CwwG_G
Cuts at: 654 1022 1292 1537 1891 654
Size: 368 270 245 354 3166
Fragments arranged by size:
3166 368 354 270 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 430 1986 2119 2578 2787 430
Size: 1556 133 459 209 2046
Fragments arranged by size:
2046 1556 459 209 133
TatI w'GTAC_w
Cuts at: 914 3918 914
Size: 3004 1399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3920 3952 3920
Size: 32 4371
Tth111I GACn'n_nGTC
Cuts at: 2200 2200
Size: 4403
XbaI T'CTAG_A
Cuts at: 1434 1434
Size: 4403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1898 1898
Size: 4403
XmaI C'CCGG_G
Cuts at: 1163 1423 1163
Size: 260 4143
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI