################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AviII AvrII BbrPI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiHKAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI DraI DraIII Eco47III Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HpaI KpnI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SacI SalI SbfI ScaI SexAI SfiI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AatII 532 GACGTC GACGT^C AflIII 1322 ACACGT A^C[GA][CT]GT AhdI 532 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 943 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 532 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1073 TGGCCA TGG^CCA BamHI 1169 GGATCC G^GATCC BanI 1216 GGCGCC G^G[CT][GA]CC BanII 129 GGGCTC G[GA]GC[CT]^C BbeI 1216 GGCGCC GGCGC^C BbsI 529 GAAGAC GAAGAC(2/6) BcgI 1040 CGAGTGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 844 AGATCT A^GATCT BmyI 129 GGGCTC G[AGT]GC[ACT]^C BpmI 1159 CTGGAG CTGGAG(16/14) BpuAI 529 GAAGAC GAAGAC(2/6) BsaI 17 GAGACC GGTCTC(1/5) BsaWI 1121 ACCGGA [AT]^CCGG[AT] BsiEI 436 CGGCCG CG[GA][CT]^CG Bsp1286I 129 GGGCTC G[AGT]GC[ACT]^C BspMI 1046 ACCTGC ACCTGC(4/8) BsrBI 226 CCGCTC CCGCTC(-3/-3) BstBI 729 TTCGAA TT^CGAA CpoI 428 CGGTCCG CG^G[AT]CCG DrdI 580 GACCAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 436 CGGCCG C^GGCCG Eam1105I 532 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 818 GAAGAG CTCTTC(1/4) EclXI 436 CGGCCG C^GGCCG Eco52I 436 CGGCCG C^GGCCG EcoRV 70 GATATC GAT^ATC EcoT14I 141 CCAAGG C^C[AT][AT]GG EcoT22I 791 ATGCAT ATGCA^T HgaI 475 GACGC GACGC(5/10) HindIII 43 AAGCTT A^AGCTT KasI 1216 GGCGCC G^GCGCC Ksp632I 818 GAAGAG CTCTTC(1/4) KspI 887 CCGCGG CCGC^GG MluNI 1073 TGGCCA TGG^CCA MscI 1073 TGGCCA TGG^CCA MseI 593 TTAA T^TAA MslI 88 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1216 GGCGCC GG^CGCC NsiI 791 ATGCAT ATGCA^T NspI 662 GCATGT [GA]CATG^[CT] NspV 729 TTCGAA TT^CGAA PaeR7I 153 CTCGAG C^TCGAG PflMI 465 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 809 GAGTC GAGTC(4/5) PshAI 764 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1249 CTGCAG CTGCA^G PvuII 124 CAGCTG CAG^CTG RsrII 428 CGGTCCG CG^G[AT]CCG SacII 887 CCGCGG CCGC^GG SapI 818 GAAGAGC GCTCTTC(1/4) SfcI 1249 CTGCAG C^T[GA][CT]AG SfoI 1216 GGCGCC GGC^GCC SfuI 729 TTCGAA TT^CGAA SgrAI 675 CACCGGCG C[GA]^CCGG[CT]G SmlI 153 CTCGAG C^T[CT][GA]AG StyI 141 CCAAGG C^C[AT][AT]GG TfiI 570 GAATC G^A[AT]TC Tru9I 593 TTAA T^TAA TspRI 290 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 465 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1148 CCAGCCCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 153 CTCGAG C^TCGAG XmnI 943 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 879 GAATTT [GA]^AATT[CT] 1096 GAATTT AcyI 532 GACGTC G[GA]^CG[CT]C 1216 GGCGCC ApoI 879 GAATTT [GA]^AATT[CT] 1096 GAATTT BsaHI 532 GACGTC G[GA]^CG[CT]C 1216 GGCGCC BseRI 799 CTCCTC GAGGAG(10/8) 1363 GAGGAG BsmBI 1005 CGTCTC CGTCTC(1/5) 762 GAGACG BsmI 781 GAATGC GAATGC(1/-1) 453 GCATTC DraII 144 AGGACCC [GA]G^G[ACGT]CC[CT] 248 AGGACCC DsaI 465 CCACGG C^C[GA][CT]GG 887 CCGCGG EcoO109I 144 AGGACCC [GA]G^G[ACGT]CC[CT] 248 AGGACCC Hin1I 532 GACGTC G[GA]^CG[CT]C 1216 GGCGCC HincII 265 GTCAAC GT[CT]^[GA]AC 670 GTCAAC HindII 265 GTCAAC GT[CT]^[GA]AC 670 GTCAAC HinfI 570 GAATC G^A[ACGT]TC 809 GAGTC NaeI 352 GCCGGC GCC^GGC 434 GCCGGC NgoMIV 352 GCCGGC G^CCGGC 434 GCCGGC PpuMI 144 AGGACCC [GA]G^G[AT]CC[CT] 248 AGGACCC SfaNI 550 GATGC GCATC(5/9) 793 GCATC Tsp509I 880 AATT ^AATT 1097 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI AvaII BbvI BcnI BfaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TseI Tsp45I TthHB8I XhoII