Restriction map for pF1KB9957
 (Linear) (Six-base) MAPSORT of: KIBB9957.seq  Check: 7721  from: 1  to: 1614



KIBB9957.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1315    1386    1614 
   Size:       1315      71     228

  Fragments arranged by size: 

                1315     228      71
 
AccI GT'mk_AC

Cuts at:      0     954    1164    1614 
   Size:        954     210     450

  Fragments arranged by size: 

                 954     450     210
 
Acc65I G'GTAC_C

Cuts at:      0    1257    1614 
   Size:       1257     357
 
AclI AA'CG_TT

Cuts at:      0    1107    1614 
   Size:       1107     507
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     194     368     424    1042    1614 
   Size:        143      51     174      56     618     572

  Fragments arranged by size: 

                 618     572     174     143      56      51
 
AgeI A'CCGG_T

Cuts at:      0     101    1614 
   Size:        101    1513
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1212    1614 
   Size:       1212     402
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30    1186    1614 
   Size:         30    1156     428

  Fragments arranged by size: 

                1156     428      30
 
ApoI r'AATT_y

Cuts at:      0     891    1043    1593    1614 
   Size:        891     152     550      21

  Fragments arranged by size: 

                 891     550     152      21
 
BanI G'GyrC_C

Cuts at:      0    1028    1257    1614 
   Size:       1028     229     357

  Fragments arranged by size: 

                1028     357     229
 
BanII G_rGCy'C

Cuts at:      0    1362    1578    1614 
   Size:       1362     216      36

  Fragments arranged by size: 

                1362     216      36
 
BbeI G_GCGC'C

Cuts at:      0    1032    1614 
   Size:       1032     582
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1372    1380    1614 
   Size:       1372       8     234

  Fragments arranged by size: 

                1372     234       8
 
BglII A'GATC_T

Cuts at:      0     399     540    1614 
   Size:        399     141    1074

  Fragments arranged by size: 

                1074     399     141
 
Bme1580I G_kGCm'C

Cuts at:      0     441    1614 
   Size:        441    1173
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     294    1614 
   Size:        294    1320
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706    1442    1592    1614 
   Size:        706     736     150      22

  Fragments arranged by size: 

                 736     706     150      22
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     536    1012    1405    1614 
   Size:        536     476     393     209

  Fragments arranged by size: 

                 536     476     393     209
 
BsaHI Gr'CG_yC

Cuts at:      0    1029    1614 
   Size:       1029     585
 
BsaWI w'CCGG_w

Cuts at:      0     101     443    1614 
   Size:        101     342    1171

  Fragments arranged by size: 

                1171     342     101
 
BseYI C'CCAG_C

Cuts at:      0    1320    1614 
   Size:       1320     294
 
BsiHKAI G_wGCw'C

Cuts at:      0     307    1578    1614 
   Size:        307    1271      36

  Fragments arranged by size: 

                1271     307      36
 
BsmI GAATG_Cn'

Cuts at:      0     359    1614 
   Size:        359    1255
 
Bsp1286I G_dGCh'C

Cuts at:      0     307     441    1362    1578    1614 
   Size:        307     134     921     216      36

  Fragments arranged by size: 

                 921     307     216     134      36
 
BspEI T'CCGG_A

Cuts at:      0     443    1614 
   Size:        443    1171
 
BspHI T'CATG_A

Cuts at:      0     627    1614 
   Size:        627     987
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1315    1386    1614 
   Size:       1315      71     228

  Fragments arranged by size: 

                1315     228      71
 
BsrDI GCAATG_nn'

Cuts at:      0    1199    1520    1614 
   Size:       1199     321      94

  Fragments arranged by size: 

                1199     321      94
 
BsrFI r'CCGG_y

Cuts at:      0     101    1614 
   Size:        101    1513
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1060    1614 
   Size:       1060     554
 
BstBI TT'CG_AA

Cuts at:      0    1590    1614 
   Size:       1590      24
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1355    1614 
   Size:       1355     259
 
BstYI r'GATC_y

Cuts at:      0     399     540    1316    1614 
   Size:        399     141     776     298

  Fragments arranged by size: 

                 776     399     298     141
 
BstZ17I GTA'TAC

Cuts at:      0     955    1165    1614 
   Size:        955     210     449

  Fragments arranged by size: 

                 955     449     210
 
DraI TTT'AAA

Cuts at:      0     336    1614 
   Size:        336    1278
 
EarI CTCTTCn'nnn_

Cuts at:      0    1349    1614 
   Size:       1349     265
 
EcoICRI GAG'CTC

Cuts at:      0    1576    1614 
   Size:       1576      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     194     368     424     706    1042    1442    1592 
   Size:        143      51     174      56     282     336     400     150

Cuts at:   1592    1614 
   Size:         22

  Fragments arranged by size: 

                 400     336     282     174     150     143      56      51
                  22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     445    1614 
   Size:        445    1169
 
HaeII r_GCGC'y

Cuts at:      0    1032    1614 
   Size:       1032     582
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1344    1376    1440    1472    1614 
   Size:       1344      32      64      32     142

  Fragments arranged by size: 

                1344     142      64      32      32
 
HindIII A'AGCT_T

Cuts at:      0     176    1614 
   Size:        176    1438
 
KasI G'GCGC_C

Cuts at:      0    1028    1614 
   Size:       1028     586
 
KpnI G_GTAC'C

Cuts at:      0    1261    1614 
   Size:       1261     353
 
MfeI C'AATT_G

Cuts at:      0     587    1614 
   Size:        587    1027
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     332    1529    1614 
   Size:        332    1197      85

  Fragments arranged by size: 

                1197     332      85
 
MslI CAynn'nnrTG

Cuts at:      0      85    1614 
   Size:         85    1529
 
NarI GG'CG_CC

Cuts at:      0    1029    1614 
   Size:       1029     585
 
NspI r_CATG'y

Cuts at:      0     429    1548    1614 
   Size:        429    1119      66

  Fragments arranged by size: 

                1119     429      66
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1327    1614 
   Size:       1327     287
 
PsiI TTA'TAA

Cuts at:      0    1073    1614 
   Size:       1073     541
 
PstI C_TGCA'G

Cuts at:      0    1191    1614 
   Size:       1191     423
 
SacI G_AGCT'C

Cuts at:      0    1578    1614 
   Size:       1578      36
 
ScaI AGT'ACT

Cuts at:      0     878    1614 
   Size:        878     736
 
SfcI C'TryA_G

Cuts at:      0     452     692    1187    1274    1614 
   Size:        452     240     495      87     340

  Fragments arranged by size: 

                 495     452     340     240      87
 
SfoI GGC'GCC

Cuts at:      0    1030    1614 
   Size:       1030     584
 
TatI w'GTAC_w

Cuts at:      0     876    1177    1614 
   Size:        876     301     437

  Fragments arranged by size: 

                 876     437     301
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     200     232    1614 
   Size:        200      32    1382

  Fragments arranged by size: 

                1382     200      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     731    1614 
   Size:        731     883
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AgeI     AlfI    AlwNI
     ApoI     BanI    BanII     BbeI     BbsI    BglII Bme1580I     BmrI
     BpmI     BsaI    BsaHI    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I     DraI     EarI  EcoICRI  Eco57MI    EcoNI    HaeII
    Hin4I  HindIII     KasI     KpnI     MfeI     MmeI     MslI     NarI
     NspI    PflMI     PsiI     PstI     SacI     ScaI     SfcI     SfoI
     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII   AflIII     AhdI     AleI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI    BsmBI    BsrBI    BsrGI   BssHII    BssSI
   BstEII   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     MluI     MscI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com