Restriction map for pF1KB5994
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AgeI        AhdI        AlwNI       
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvaI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HaeIII      
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Van91I      XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AclI         1587  AACGTT               AA^CGTT
AflIII        226  ACATGT               A^C[GA][CT]GT
Alw44I        441  GTGCAC               G^TGCAC
AlwI         1165  GATCC                GGATC(4/5)
AocI         1817  CCTTAGG              CC^T[ACGT]AGG
ApaLI         441  GTGCAC               G^TGCAC
AspHI         441  GTGCAC               G[AT]GC[AT]^C
AspI         1483  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1359  CCTAGG               C^CTAGG
BbvCI        1030  GCTGAGG              CCTCAGC(-5/-2)
BcnI          525  CCCGG                CC^[GC]GG
BlnI         1359  CCTAGG               C^CTAGG
BmyI          441  GTGCAC               G[AGT]GC[ACT]^C
BpmI          885  CTCCAG               CTGGAG(16/14)
BseRI        1780  GAGGAG               GAGGAG(10/8)
BsiHKAI       441  GTGCAC               G[AT]GC[AT]^C
BsiYI         408  CCCCCGATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          408  CCCCCGATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      441  GTGCAC               G[AGT]GC[ACT]^C
BspLU11I      226  ACATGT               A^CATGT
BspMI        1768  ACCTGC               ACCTGC(4/8)
BsrDI         939  CATTGC               GCAATG(2/0)
BstEII          9  GGTAACC              G^GT[ACGT]ACC
BstPI           9  GGTAACC              G^GT[ACGT]ACC
BstXI         681  CCAAAAATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1817  CCTTAGG              CC^T[ACGT]AGG
CfoI         1288  GCGC                 GCG^C
Eco81I       1817  CCTTAGG              CC^T[ACGT]AGG
EcoO65I         9  GGTAACC              G^GT[ACGT]ACC
HapII         526  CCGG                 C^CGG
HhaI         1288  GCGC                 GCG^C
HinP1I       1288  GCGC                 G^CGC
HincII       1326  GTCAAC               GT[CT]^[GA]AC
HindII       1326  GTCAAC               GT[CT]^[GA]AC
HpaII         526  CCGG                 C^CGG
MspI          526  CCGG                 C^CGG
NciI          525  CCCGG                CC^[GC]GG
NcoI          810  CCATGG               C^CATGG
NdeI         1127  CATATG               CA^TATG
PflFI        1483  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     1587  AACGTT               AA^CGTT
PstI         1160  CTGCAG               CTGCA^G
PvuII         245  CAGCTG               CAG^CTG
SapI         1216  GAAGAGC              GCTCTTC(1/4)
SfcI         1160  CTGCAG               C^T[GA][CT]AG
Tth111I      1483  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1140  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI         1157  CCGC                 CCGC(-3/-1)
              844  GCGG                
AfaI         1272  GTAC                 GT^AC
             1315  GTAC                
AvaII         846  GGACC                G^G[AT]CC
             1437  GGTCC               
BfaI         1141  CTAG                 C^TAG
             1360  CTAG                
BglII         780  AGATCT               A^GATCT
              816  AGATCT              
BspHI         453  TCATGA               T^CATGA
             1170  TCATGA              
BstNI          53  CCAGG                CC^[AT]GG
             1050  CCTGG               
Cfr13I        846  GGACC                G^G[ACGT]CC
             1437  GGTCC               
DraI          103  TTTAAA               TTT^AAA
             1201  TTTAAA              
DsaI          810  CCATGG               C^C[GA][CT]GG
             1433  CCGTGG              
EcoRII         53  CCAGG                ^CC[AT]GG
             1050  CCTGG               
MaeI         1141  CTAG                 C^TAG
             1360  CTAG                
MaeII        1562  ACGT                 A^CGT
             1588  ACGT                
MspA1I        245  CAGCTG               C[AC]G^C[GT]G
             1157  CCGCTG              
MvaI           53  CCAGG                CC^[AT]GG
             1050  CCTGG               
NspI          226  ACATGT               [GA]CATG^[CT]
             1055  GCATGT              
RcaI          453  TCATGA               T^CATGA
             1170  TCATGA              
RsaI         1272  GTAC                 GT^AC
             1315  GTAC                
Sau96I        846  GGACC                G^G[ACGT]CC
             1437  GGTCC               
SmlI          664  CTCAAG               C^T[CT][GA]AG
             1350  CTCAAG              
TfiI          877  GATTC                G^A[AT]TC
             1507  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AcsI        AluI        ApoI        BbsI        BbvI        
BpuAI       BsaI        BsaJI       BsgI        BsmAI       
BsmI        BsrI        BssKI       BstF5I      BstYI       
Cac8I       DdeI        DpnII       EarI        EcoT14I     
Fnu4HI      FokI        HinfI       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MwoI        NdeII       
NlaIII      PleI        Sau3AI      ScrFI       SfaNI       
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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