Restriction map for pF1KB5994
 (Linear) (Six-base) MAPSORT of: KIBB5994.seq  Check: 3165  from: 1  to: 1827



KIBB5994.orf

 With 182 enzymes: SgfI * 

                        July 10, 2012 13:28  ..

AclI AA'CG_TT

Cuts at:      0    1588    1827 
   Size:       1588     239
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     916    1415    1827 
   Size:        148     768     499     412

  Fragments arranged by size: 

                 768     499     412     148
 
AflIII A'CryG_T

Cuts at:      0     226    1827 
   Size:        226    1601
 
AleI CACnn'nnGTG

Cuts at:      0      83    1827 
   Size:         83    1744
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1626    1827 
   Size:       1626     201
 
ApaLI G'TGCA_C

Cuts at:      0     441    1827 
   Size:        441    1386
 
ApoI r'AATT_y

Cuts at:      0     123     214     685    1198    1227    1276    1827 
   Size:        123      91     471     513      29      49     551

  Fragments arranged by size: 

                 551     513     471     123      91      49      29
 
AvrII C'CTAG_G

Cuts at:      0    1359    1827 
   Size:       1359     468
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     510    1038    1427    1827 
   Size:        510     528     389     400

  Fragments arranged by size: 

                 528     510     400     389
 
BbvCI CC'TCA_GC

Cuts at:      0    1031    1827 
   Size:       1031     796
 
BglII A'GATC_T

Cuts at:      0     780     816    1827 
   Size:        780      36    1011

  Fragments arranged by size: 

                1011     780      36
 
Bme1580I G_kGCm'C

Cuts at:      0     445    1827 
   Size:        445    1382
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     870    1827 
   Size:        870     957
 
Bpu10I CC'TnA_GC

Cuts at:      0     727    1031    1827 
   Size:        727     304     796

  Fragments arranged by size: 

                 796     727     304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649    1335    1827 
   Size:        649     686     492

  Fragments arranged by size: 

                 686     649     492
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     414    1353    1759    1827 
   Size:        414     939     406      68

  Fragments arranged by size: 

                 939     414     406      68
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1795    1827 
   Size:       1795      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1436    1485    1637    1827 
   Size:       1436      49     152     190

  Fragments arranged by size: 

                1436     190     152      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     445    1827 
   Size:        445    1382
 
BsmI GAATG_Cn'

Cuts at:      0     376     808     907    1180    1290    1412    1827 
   Size:        376     432      99     273     110     122     415

  Fragments arranged by size: 

                 432     415     376     273     122     110      99
 
Bsp1286I G_dGCh'C

Cuts at:      0     445    1827 
   Size:        445    1382
 
BspHI T'CATG_A

Cuts at:      0     453    1170    1827 
   Size:        453     717     657

  Fragments arranged by size: 

                 717     657     453
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1777    1827 
   Size:       1777      50
 
BsrDI GCAATG_nn'

Cuts at:      0     938    1827 
   Size:        938     889
 
BstEII G'GTnAC_C

Cuts at:      0       9    1827 
   Size:          9    1818
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     688    1827 
   Size:        688    1139
 
BstYI r'GATC_y

Cuts at:      0     780     816    1164    1827 
   Size:        780      36     348     663

  Fragments arranged by size: 

                 780     663     348      36
 
Bsu36I CC'TnA_GG

Cuts at:      0    1818    1827 
   Size:       1818       9
 
BtgI C'CryG_G

Cuts at:      0     810    1433    1827 
   Size:        810     623     394

  Fragments arranged by size: 

                 810     623     394
 
BtsI GCAGTG_nn'

Cuts at:      0     175     443     933    1827 
   Size:        175     268     490     894

  Fragments arranged by size: 

                 894     490     268     175
 
DraI TTT'AAA

Cuts at:      0     105    1203    1827 
   Size:        105    1098     624

  Fragments arranged by size: 

                1098     624     105
 
EarI CTCTTCn'nnn_

Cuts at:      0     314     458    1211    1827 
   Size:        314     144     753     616

  Fragments arranged by size: 

                 753     616     314     144
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     859    1827 
   Size:        859     968
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     870     916    1415    1827 
   Size:        148     722      46     499     412

  Fragments arranged by size: 

                 722     499     412     148      46
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     527    1062    1827 
   Size:        527     535     765

  Fragments arranged by size: 

                 765     535     527
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     868     900    1414    1446    1827 
   Size:        868      32     514      32     381

  Fragments arranged by size: 

                 868     514     381      32      32
 
HincII GTy'rAC

Cuts at:      0    1328    1827 
   Size:       1328     499
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     902    1357    1827 
   Size:        902     455     470

  Fragments arranged by size: 

                 902     470     455
 
MslI CAynn'nnrTG

Cuts at:      0      83     389     458    1827 
   Size:         83     306      69    1369

  Fragments arranged by size: 

                1369     306      83      69
 
MspA1I CmG'CkG

Cuts at:      0     247    1159    1827 
   Size:        247     912     668

  Fragments arranged by size: 

                 912     668     247
 
NcoI C'CATG_G

Cuts at:      0     810    1827 
   Size:        810    1017
 
NdeI CA'TA_TG

Cuts at:      0    1128    1827 
   Size:       1128     699
 
NspI r_CATG'y

Cuts at:      0     230    1059    1827 
   Size:        230     829     768

  Fragments arranged by size: 

                 829     768     230
 
PciI A'CATG_T

Cuts at:      0     226    1827 
   Size:        226    1601
 
PstI C_TGCA'G

Cuts at:      0    1164    1827 
   Size:       1164     663
 
PvuII CAG'CTG

Cuts at:      0     247    1827 
   Size:        247    1580
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1211    1827 
   Size:       1211     616
 
SfcI C'TryA_G

Cuts at:      0    1160    1827 
   Size:       1160     667
 
SmlI C'TyrA_G

Cuts at:      0     664    1350    1827 
   Size:        664     686     477

  Fragments arranged by size: 

                 686     664     477
 
StyI C'CwwG_G

Cuts at:      0     134     810    1359    1511    1682    1827 
   Size:        134     676     549     152     171     145

  Fragments arranged by size: 

                 676     549     171     152     145     134
 
TatI w'GTAC_w

Cuts at:      0    1271    1827 
   Size:       1271     556
 
Tth111I GACn'n_nGTC

Cuts at:      0    1486    1827 
   Size:       1486     341
 
XbaI T'CTAG_A

Cuts at:      0    1140    1827 
   Size:       1140     687
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AleI     AlfI    ApaLI     ApoI    AvrII
     BbsI    BbvCI    BglII Bme1580I     BpmI   Bpu10I    BpuEI     BsaI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI     EarI
     EciI  Eco57MI     FalI    Hin4I   HincII     MmeI     MslI   MspA1I
     NcoI     NdeI     NspI     PciI     PstI    PvuII     SapI     SfcI
     SmlI     StyI     TatI  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI     XcmI     XhoI     XmaI     XmnI     ZraI
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