Restriction map for pF1KB6754
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaHI       BsaI        BseAI       
BsgI        BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrI        BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       FauI        Fnu4HI      FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        ItaI        KasI        KpnI        KspI        
MaeI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        MvnI        
NarI        NciI        NcoI        NdeI        NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        TseI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AfaI          154  GTAC                 GT^AC
AluI          260  AGCT                 AG^CT
AlwNI         379  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         381  GGACC                G^G[AT]CC
BamHI         342  GGATCC               G^GATCC
BbsI          397  GTCTTC               GAAGAC(2/6)
BclI          134  TGATCA               T^GATCA
BmyI           34  GGGCAC               G[AGT]GC[ACT]^C
BpmI           64  CTGGAG               CTGGAG(16/14)
BpuAI         397  GTCTTC               GAAGAC(2/6)
BsaAI          28  TACGTG               [CT]AC^GT[GA]
BsaBI         325  GATGTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         338  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI          81  ACCGGA               [AT]^CCGG[AT]
BseRI         233  CTCCTC               GAGGAG(10/8)
BsiEI          79  CGACCG               CG[GA][CT]^CG
Bsp1286I       34  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      153  TGTACA               T^GTACA
BspHI         131  TCATGA               T^CATGA
BsrFI         251  GCCGGC               [GA]^CCGG[CT]
BsrGI         153  TGTACA               T^GTACA
BssSI          55  CACGAG               CACGAG(-5/-1)
BstF5I        216  GGATG                GGATG(2/0)
BstYI         342  GGATCC               [GA]^GATC[CT]
Cac8I         251  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I        251  GCCGGC               [GA]^CCGG[CT]
DraII         380  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI           51  CGGCCA               [CT]^GGCC[GA]
EarI          180  GAAGAG               CTCTTC(1/4)
EcoO109I      380  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       338  CCAAGG               C^C[AT][AT]GG
EcoT22I       221  ATGCAT               ATGCA^T
FbaI          134  TGATCA               T^GATCA
FokI          216  GGATG                GGATG(9/13)
Ksp632I       180  GAAGAG               CTCTTC(1/4)
MaeII          29  ACGT                 A^CGT
MamI          325  GATGTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          342  GGATCC               [GA]^GATC[CT]
MwoI           44  GCAAGTTCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI          251  GCCGGC               GCC^GGC
NgoMIV        251  GCCGGC               G^CCGGC
NlaIII        132  CATG                 CATG^
NsiI          221  ATGCAT               ATGCA^T
PpuMI         380  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          131  TCATGA               T^CATGA
RsaI          154  GTAC                 GT^AC
SfaNI         220  GATGC                GCATC(5/9)
SspBI         153  TGTACA               T^GTACA
StyI          338  CCAAGG               C^C[AT][AT]GG
TaqI           78  TCGA                 T^CGA
Tsp45I          8  GTGAC                ^GT[GC]AC
TspRI         198  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        78  TCGA                 T^CGA
XhoII         342  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          343  GATCC                GGATC(4/5)
              342  GGATC               
BssKI          63  CCTGG                ^CC[ACGT]GG
              378  CCAGG               
BstNI          63  CCTGG                CC^[AT]GG
              378  CCAGG               
Cfr13I        249  GGGCC                G^G[ACGT]CC
              381  GGACC               
DpnII         135  GATC                 ^GATC
              343  GATC                
EcoRII         63  CCTGG                ^CC[AT]GG
              378  CCAGG               
HapII          82  CCGG                 C^CGG
              252  CCGG                
HpaII          82  CCGG                 C^CGG
              252  CCGG                
MaeIII          8  GTGAC                ^GT[ACGT]AC
               23  GTTAC               
MboI          135  GATC                 ^GATC
              343  GATC                
MboII         180  GAAGA                GAAGA(8/7)
              398  TCTTC               
MseI           94  TTAA                 T^TAA
              428  TTAA                
MspI           82  CCGG                 C^CGG
              252  CCGG                
MvaI           63  CCTGG                CC^[AT]GG
              378  CCAGG               
NdeII         135  GATC                 ^GATC
              343  GATC                
NlaIV         313  GGTTCC               GG[ACGT]^[ACGT]CC
              342  GGATCC              
Sau3AI        135  GATC                 ^GATC
              343  GATC                
Sau96I        249  GGGCC                G^G[ACGT]CC
              381  GGACC               
ScrFI          63  CCTGG                CC^[ACGT]GG
              378  CCAGG               
Tru9I          94  TTAA                 T^TAA
              428  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

HaeIII      MnlI        Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com