Restriction map for
pF1KB7508
(Linear) (Six-base) MAPSORT of: KIBB7508.seq Check: 668 from: 1 to: 726
KIBB7508.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 553 726
Size: 553 173
Acc65I G'GTAC_C
Cuts at: 0 115 726
Size: 115 611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 477 726
Size: 367 110 249
Fragments arranged by size:
367 249 110
AflIII A'CryG_T
Cuts at: 0 180 726
Size: 180 546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 680 712 726
Size: 680 32 14
Fragments arranged by size:
680 32 14
AlwNI CAG_nnn'CTG
Cuts at: 0 231 381 438 477 726
Size: 231 150 57 39 249
Fragments arranged by size:
249 231 150 57 39
AvaI C'yCGr_G
Cuts at: 0 195 636 726
Size: 195 441 90
Fragments arranged by size:
441 195 90
BanI G'GyrC_C
Cuts at: 0 115 189 726
Size: 115 74 537
Fragments arranged by size:
537 115 74
BanII G_rGCy'C
Cuts at: 0 590 726
Size: 590 136
BbeI G_GCGC'C
Cuts at: 0 193 726
Size: 193 533
BciVI GTATCCnnnnn_n'
Cuts at: 0 110 726
Size: 110 616
BglI GCCn_nnn'nGGC
Cuts at: 0 340 726
Size: 340 386
BmrI ACTGGGnnnn_n'
Cuts at: 0 549 726
Size: 549 177
Bpu10I CC'TnA_GC
Cuts at: 0 602 726
Size: 602 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 726
Size: 541 185
BsaHI Gr'CG_yC
Cuts at: 0 190 726
Size: 190 536
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 99 129 680 710 726
Size: 99 30 551 30 16
Fragments arranged by size:
551 99 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 324 626 726
Size: 324 302 100
Fragments arranged by size:
324 302 100
BseYI C'CCAG_C
Cuts at: 0 477 726
Size: 477 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 726
Size: 427 299
Bsp1286I G_dGCh'C
Cuts at: 0 590 726
Size: 590 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 259 446 553 726
Size: 259 187 107 173
Fragments arranged by size:
259 187 173 107
BsrBI CCG'CTC
Cuts at: 0 426 726
Size: 426 300
BsrFI r'CCGG_y
Cuts at: 0 278 446 726
Size: 278 168 280
Fragments arranged by size:
280 278 168
BssSI C'ACGA_G
Cuts at: 0 589 726
Size: 589 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 251 438 726
Size: 251 187 288
Fragments arranged by size:
288 251 187
BtsI GCAGTG_nn'
Cuts at: 0 213 726
Size: 213 513
EaeI y'GGCC_r
Cuts at: 0 222 362 559 726
Size: 222 140 197 167
Fragments arranged by size:
222 197 167 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 477 726
Size: 367 110 249
Fragments arranged by size:
367 249 110
EcoO109I rG'GnC_Cy
Cuts at: 0 435 726
Size: 435 291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 535 726
Size: 535 191
FspI TGC'GCA
Cuts at: 0 541 726
Size: 541 185
HaeII r_GCGC'y
Cuts at: 0 193 410 726
Size: 193 217 316
Fragments arranged by size:
316 217 193
KasI G'GCGC_C
Cuts at: 0 189 726
Size: 189 537
KpnI G_GTAC'C
Cuts at: 0 119 726
Size: 119 607
MscI TGG'CCA
Cuts at: 0 364 561 726
Size: 364 197 165
Fragments arranged by size:
364 197 165
MspA1I CmG'CkG
Cuts at: 0 170 726
Size: 170 556
NarI GG'CG_CC
Cuts at: 0 190 726
Size: 190 536
NspI r_CATG'y
Cuts at: 0 184 726
Size: 184 542
PciI A'CATG_T
Cuts at: 0 180 726
Size: 180 546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 680 712 726
Size: 680 32 14
Fragments arranged by size:
680 32 14
PpuMI rG'GwC_Cy
Cuts at: 0 435 726
Size: 435 291
PspXI vC'TCGA_Gb
Cuts at: 0 636 726
Size: 636 90
PstI C_TGCA'G
Cuts at: 0 68 206 434 550 726
Size: 68 138 228 116 176
Fragments arranged by size:
228 176 138 116 68
SfcI C'TryA_G
Cuts at: 0 64 202 430 498 546 726
Size: 64 138 228 68 48 180
Fragments arranged by size:
228 180 138 68 64 48
SfoI GGC'GCC
Cuts at: 0 191 726
Size: 191 535
SmaI CCC'GGG
Cuts at: 0 197 726
Size: 197 529
SmlI C'TyrA_G
Cuts at: 0 520 636 726
Size: 520 116 90
Fragments arranged by size:
520 116 90
StuI AGG'CCT
Cuts at: 0 334 726
Size: 334 392
StyI C'CwwG_G
Cuts at: 0 386 726
Size: 386 340
XhoI C'TCGA_G
Cuts at: 0 636 726
Size: 636 90
XmaI C'CCGG_G
Cuts at: 0 195 726
Size: 195 531
Enzymes that do cut:
AarI Acc65I AcuI AflIII AloI AlwNI AvaI BanI
BanII BbeI BciVI BglI BmrI Bpu10I BpuEI BsaHI
BsaXI BseRI BseYI BsgI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BtsI EaeI Eco57MI EcoO109I FalI FspI
HaeII KasI KpnI MscI MspA1I NarI NspI PciI
PpiI PpuMI PspXI PstI SfcI SfoI SmaI SmlI
StuI StyI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmtI BplI BpmI BsaI BsaAI
BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspAI Hin4I HincII HindIII HpaI MfeI MluI
MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI