(Circular) (Six-base) MAPSORT of: pF1KB7865.seq Check: 71 from: 1 to: 4784 pF1KB7865 4784 bp With 182 enzymes: SgfI * October 7, 2009 14:24 .. AccI GT'mk_AC Cuts at: 995 1822 995 Size: 827 3957 Acc65I G'GTAC_C Cuts at: 1800 1800 Size: 4784 AclI AA'CG_TT Cuts at: 4624 4624 Size: 4784 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2609 3041 3788 4056 2609 Size: 432 747 268 3337 Fragments arranged by size: 3337 747 432 268 AflIII A'CryG_T Cuts at: 1640 1742 2136 3240 4217 1640 Size: 102 394 1104 977 2207 Fragments arranged by size: 2207 1104 977 394 102 AgeI A'CCGG_T Cuts at: 809 3172 809 Size: 2363 2421 AlwNI CAG_nnn'CTG Cuts at: 714 1539 2089 3656 714 Size: 825 550 1567 1842 Fragments arranged by size: 1842 1567 825 550 ApaI G_GGCC'C Cuts at: 827 827 Size: 4784 ApaLI G'TGCA_C Cuts at: 3554 3554 Size: 4784 ApoI r'AATT_y Cuts at: 1788 4137 4393 1788 Size: 2349 256 2179 Fragments arranged by size: 2349 2179 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4784 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4784 AvaI C'yCGr_G Cuts at: 1804 1804 Size: 4784 BamHI G'GATC_C Cuts at: 1809 1809 Size: 4784 BanI G'GyrC_C Cuts at: 577 1800 2214 2462 2497 577 Size: 1223 414 248 35 2864 Fragments arranged by size: 2864 1223 414 248 35 BanII G_rGCy'C Cuts at: 163 275 346 785 827 970 1579 1798 2828 Size: 112 71 439 42 143 609 219 1030 Cuts at: 2828 163 Size: 2119 Fragments arranged by size: 2119 1030 609 439 219 143 112 71 42 BbeI G_GCGC'C Cuts at: 2218 2466 2218 Size: 248 4536 BbsI GAAGACnn'nnnn_ Cuts at: 282 595 1739 282 Size: 313 1144 3327 Fragments arranged by size: 3327 1144 313 BciVI GTATCCnnnnn_n' Cuts at: 2682 3443 4784 2682 Size: 761 1341 2682 Fragments arranged by size: 2682 1341 761 BclI T'GATC_A Cuts at: 479 2304 479 Size: 1825 2959 BglII A'GATC_T Cuts at: 2299 4336 2299 Size: 2037 2747 BlpI GC'TnA_GC Cuts at: 157 1896 157 Size: 1739 3045 Bme1580I G_kGCm'C Cuts at: 580 827 2409 2502 3558 580 Size: 247 1582 93 1056 1806 Fragments arranged by size: 1806 1582 1056 247 93 BmgBI CAC'GTC Cuts at: 125 125 Size: 4784 BmrI ACTGGGnnnn_n' Cuts at: 1336 1662 1996 2077 2412 4556 1336 Size: 326 334 81 335 2144 1564 Fragments arranged by size: 2144 1564 335 334 326 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 661 3151 661 Size: 2490 2294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 115 256 1445 115 Size: 141 1189 3454 Fragments arranged by size: 3454 1189 141 Bpu10I CC'TnA_GC Cuts at: 145 145 Size: 4784 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 367 1960 2839 3331 3629 367 Size: 1593 879 492 298 1522 Fragments arranged by size: 1593 1522 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4488 4488 Size: 4784 BsaAI yAC'GTr Cuts at: 2137 2137 Size: 4784 BsaBI GATnn'nnATC Cuts at: 6 2298 4335 6 Size: 2292 2037 455 Fragments arranged by size: 2292 2037 455 BsaHI Gr'CG_yC Cuts at: 1055 2215 2463 3165 4661 1055 Size: 1160 248 702 1496 1178 Fragments arranged by size: 1496 1178 1160 702 248 BsaWI w'CCGG_w Cuts at: 440 809 1973 2494 3172 3446 3593 4004 440 Size: 369 1164 521 678 274 147 411 1220 Fragments arranged by size: 1220 1164 678 521 411 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 328 358 1357 1387 328 Size: 30 999 30 3725 Fragments arranged by size: 3725 999 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 214 424 1039 1119 1124 1185 1282 1343 214 Size: 210 615 80 5 61 97 61 3655 Fragments arranged by size: 3655 615 210 97 80 61 61 5 BseYI C'CCAG_C Cuts at: 560 833 874 1123 1263 1388 1532 1595 2209 Size: 273 41 249 140 125 144 63 614 Cuts at: 2209 2967 3544 4040 560 Size: 758 577 496 1304 Fragments arranged by size: 1304 758 614 577 496 273 249 144 140 125 63 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1525 1525 Size: 4784 BsiEI CG_ry'CG Cuts at: 104 861 2326 2372 3580 104 Size: 757 1465 46 1208 1308 Fragments arranged by size: 1465 1308 1208 757 46 BsiHKAI G_wGCw'C Cuts at: 163 1798 2576 2766 3558 163 Size: 1635 778 190 792 1389 Fragments arranged by size: 1635 1389 792 778 190 BsmI GAATG_Cn' Cuts at: 913 1030 913 Size: 117 4667 BsmBI CGTCTCn'nnnn_ Cuts at: 232 232 Size: 4784 Bsp1286I G_dGCh'C Cuts at: 163 275 346 580 785 827 970 1579 1798 Size: 112 71 234 205 42 143 609 219 Cuts at: 1798 2409 2502 2576 2766 2828 3558 163 Size: 611 93 74 190 62 730 1389 Fragments arranged by size: 1389 730 611 609 234 219 205 190 143 112 93 74 71 62 42 BspEI T'CCGG_A Cuts at: 4004 4004 Size: 4784 BspHI T'CATG_A Cuts at: 1409 3960 1409 Size: 2551 2233 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1834 2048 2350 2731 1834 Size: 214 302 381 3887 Fragments arranged by size: 3887 381 302 214 BsrBI CCG'CTC Cuts at: 50 607 3076 3130 4614 4779 50 Size: 557 2469 54 1484 165 55 Fragments arranged by size: 2469 1484 557 165 55 54 BsrDI GCAATG_nn' Cuts at: 1706 2696 1706 Size: 990 3794 BsrFI r'CCGG_y Cuts at: 285 535 809 1495 2782 2963 3172 285 Size: 250 274 686 1287 181 209 1897 Fragments arranged by size: 1897 1287 686 274 250 209 181 BsrGI T'GTAC_A Cuts at: 611 611 Size: 4784 BssSI C'ACGA_G Cuts at: 3055 3413 3055 Size: 358 4426 BstAPI GCAn_nnn'nTGC Cuts at: 2161 2161 Size: 4784 BstBI TT'CG_AA Cuts at: 3145 3145 Size: 4784 BstEII G'GTnAC_C Cuts at: 168 1230 168 Size: 1062 3722 BstXI CCAn_nnnn'nTGG Cuts at: 676 754 676 Size: 78 4706 BstYI r'GATC_y Cuts at: 1 641 1809 2276 2299 2634 2880 3892 3978 Size: 640 1168 467 23 335 246 1012 86 Cuts at: 3978 3990 4336 1 Size: 12 346 449 Fragments arranged by size: 1168 1012 640 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 747 957 1049 1101 1212 1226 107 Size: 640 210 92 52 111 14 3665 Fragments arranged by size: 3665 640 210 111 92 52 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2734 2915 3078 2734 Size: 181 163 4440 Fragments arranged by size: 4440 181 163 BtsI GCAGTG_nn' Cuts at: 2027 2027 Size: 4784 DraI TTT'AAA Cuts at: 1783 1783 Size: 4784 DraIII CAC_nnn'GTG Cuts at: 1471 1471 Size: 4784 DrdI GACnn_nn'nnGTC Cuts at: 1239 2490 3348 1239 Size: 1251 858 2675 Fragments arranged by size: 2675 1251 858 EaeI y'GGCC_r Cuts at: 309 1046 1493 2369 2543 2934 2961 4306 309 Size: 737 447 876 174 391 27 1345 787 Fragments arranged by size: 1345 876 787 737 447 391 174 27 EagI C'GGCC_G Cuts at: 2369 2369 Size: 4784 EarI CTCTTCn'nnn_ Cuts at: 878 2807 3017 878 Size: 1929 210 2645 Fragments arranged by size: 2645 1929 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 433 445 2992 3302 3448 4594 433 Size: 12 2547 310 146 1146 623 Fragments arranged by size: 2547 1146 623 310 146 12 EcoICRI GAG'CTC Cuts at: 161 1796 161 Size: 1635 3149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 115 256 1445 2609 3041 3788 4056 115 Size: 141 1189 1164 432 747 268 843 Fragments arranged by size: 1189 1164 843 747 432 268 141 EcoO109I rG'GnC_Cy Cuts at: 456 823 1923 456 Size: 367 1100 3317 Fragments arranged by size: 3317 1100 367 EcoRI G'AATT_C Cuts at: 1788 1788 Size: 4784 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 306 342 2803 306 Size: 36 2461 2287 Fragments arranged by size: 2461 2287 36 FspI TGC'GCA Cuts at: 2565 2565 Size: 4784 HaeII r_GCGC'y Cuts at: 152 400 1226 2218 2466 3488 4467 152 Size: 248 826 992 248 1022 979 469 Fragments arranged by size: 1022 992 979 826 469 248 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 328 360 760 792 2626 2656 2658 2688 328 Size: 32 400 32 1834 30 2 30 2424 Fragments arranged by size: 2424 1834 400 32 32 30 30 2 HincII GTy'rAC Cuts at: 739 1823 739 Size: 1084 3700 KasI G'GCGC_C Cuts at: 2214 2462 2214 Size: 248 4536 KpnI G_GTAC'C Cuts at: 1804 1804 Size: 4784 MfeI C'AATT_G Cuts at: 1436 1436 Size: 4784 MluI A'CGCG_T Cuts at: 4217 4217 Size: 4784 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 672 995 1091 3455 3639 672 Size: 323 96 2364 184 1817 Fragments arranged by size: 2364 1817 323 184 96 MscI TGG'CCA Cuts at: 1048 2545 1048 Size: 1497 3287 MslI CAynn'nnrTG Cuts at: 583 1741 2773 2900 583 Size: 1158 1032 127 2467 Fragments arranged by size: 2467 1158 1032 127 MspA1I CmG'CkG Cuts at: 263 426 711 1539 1615 1626 1895 2209 2569 Size: 163 285 828 76 11 269 314 360 Cuts at: 2569 3582 3827 263 Size: 1013 245 1220 Fragments arranged by size: 1220 1013 828 360 314 285 269 245 163 76 11 NaeI GCC'GGC Cuts at: 1497 2965 1497 Size: 1468 3316 NarI GG'CG_CC Cuts at: 2215 2463 2215 Size: 248 4536 NcoI C'CATG_G Cuts at: 107 747 1049 1212 1226 107 Size: 640 302 163 14 3665 Fragments arranged by size: 3665 640 302 163 14 NgoMIV G'CCGG_C Cuts at: 1495 2963 1495 Size: 1468 3316 NspI r_CATG'y Cuts at: 495 1644 1746 1837 2337 3244 495 Size: 1149 102 91 500 907 2035 Fragments arranged by size: 2035 1149 907 500 102 91 PciI A'CATG_T Cuts at: 1640 1742 3240 1640 Size: 102 1498 3184 Fragments arranged by size: 3184 1498 102 PflMI CCAn_nnn'nTGG Cuts at: 963 963 Size: 4784 PmeI GTTT'AAAC Cuts at: 1783 1783 Size: 4784 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3923 3955 3923 Size: 32 4752 PpuMI rG'GwC_Cy Cuts at: 456 456 Size: 4784 PspOMI G'GGCC_C Cuts at: 823 823 Size: 4784 PstI C_TGCA'G Cuts at: 395 1517 1631 1831 2516 395 Size: 1122 114 200 685 2663 Fragments arranged by size: 2663 1122 685 200 114 PvuI CG_AT'CG Cuts at: 104 861 104 Size: 757 4027 PvuII CAG'CTG Cuts at: 711 1539 1615 1626 2209 2569 711 Size: 828 76 11 583 360 2926 Fragments arranged by size: 2926 828 583 360 76 11 RsrII CG'GwC_CG Cuts at: 2979 2979 Size: 4784 SacI G_AGCT'C Cuts at: 163 1798 163 Size: 1635 3149 SalI G'TCGA_C Cuts at: 1821 1821 Size: 4784 SapI GCTCTTCn'nnn_ Cuts at: 2807 3017 2807 Size: 210 4574 SbfI CC_TGCA'GG Cuts at: 1831 1831 Size: 4784 ScaI AGT'ACT Cuts at: 4301 4301 Size: 4784 SexAI A'CCwGG_T Cuts at: 616 616 Size: 4784 SfcI C'TryA_G Cuts at: 33 391 1059 1161 1513 1627 1827 2512 3505 Size: 358 668 102 352 114 200 685 993 Cuts at: 3505 3696 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 668 358 352 200 191 114 102 SfoI GGC'GCC Cuts at: 2216 2464 2216 Size: 248 4536 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4784 SmaI CCC'GGG Cuts at: 1806 1806 Size: 4784 SmlI C'TyrA_G Cuts at: 382 1939 2854 3346 3608 382 Size: 1557 915 492 262 1558 Fragments arranged by size: 1558 1557 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4784 SphI G_CATG'C Cuts at: 1837 2337 1837 Size: 500 4284 StuI AGG'CCT Cuts at: 193 853 895 193 Size: 660 42 4082 Fragments arranged by size: 4082 660 42 StyI C'CwwG_G Cuts at: 107 173 194 317 460 747 1049 1212 1226 Size: 66 21 123 143 287 302 163 14 Cuts at: 1226 1918 2272 107 Size: 692 354 2619 Fragments arranged by size: 2619 692 354 302 287 163 143 123 66 21 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2367 2500 2959 3168 2367 Size: 133 459 209 3983 Fragments arranged by size: 3983 459 209 133 TatI w'GTAC_w Cuts at: 611 1729 4299 611 Size: 1118 2570 1096 Fragments arranged by size: 2570 1118 1096 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 362 394 435 467 1357 1389 1457 1489 4301 Size: 32 41 32 890 32 68 32 2812 Cuts at: 4301 4333 362 Size: 32 813 Fragments arranged by size: 2812 890 813 68 41 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2581 2581 Size: 4784 XbaI T'CTAG_A Cuts at: 1815 1815 Size: 4784 XcmI CCAnnnn_n'nnnnTGG Cuts at: 180 2279 180 Size: 2099 2685 XmaI C'CCGG_G Cuts at: 1804 1804 Size: 4784 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI