(Circular) (Six-base) MAPSORT of: pF1KB5888.seq Check: 8172 from: 1 to: 4676 pF1KB5888 4676 bp With 182 enzymes: SgfI * October 7, 2009 14:18 .. AccI GT'mk_AC Cuts at: 887 1714 887 Size: 827 3849 Acc65I G'GTAC_C Cuts at: 1618 1692 1618 Size: 74 4602 AclI AA'CG_TT Cuts at: 4516 4516 Size: 4676 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1622 2501 2933 3680 3948 1622 Size: 879 432 747 268 2350 Fragments arranged by size: 2350 879 747 432 268 AflIII A'CryG_T Cuts at: 2028 3132 4109 2028 Size: 1104 977 2595 Fragments arranged by size: 2595 1104 977 AgeI A'CCGG_T Cuts at: 3064 3064 Size: 4676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 593 593 Size: 4676 AlwNI CAG_nnn'CTG Cuts at: 1981 3548 1981 Size: 1567 3109 ApaLI G'TGCA_C Cuts at: 3446 3446 Size: 4676 ApoI r'AATT_y Cuts at: 1660 1680 4029 4285 1660 Size: 20 2349 256 2051 Fragments arranged by size: 2349 2051 256 20 AseI AT'TA_AT Cuts at: 20 1213 20 Size: 1193 3483 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4676 AvaI C'yCGr_G Cuts at: 1696 1696 Size: 4676 BamHI G'GATC_C Cuts at: 801 1701 801 Size: 900 3776 BanI G'GyrC_C Cuts at: 1048 1618 1692 2106 2354 2389 1048 Size: 570 74 414 248 35 3335 Fragments arranged by size: 3335 570 414 248 74 35 BanII G_rGCy'C Cuts at: 903 1690 2720 903 Size: 787 1030 2859 Fragments arranged by size: 2859 1030 787 BbeI G_GCGC'C Cuts at: 2110 2358 2110 Size: 248 4428 BbsI GAAGACnn'nnnn_ Cuts at: 192 1526 192 Size: 1334 3342 BciVI GTATCCnnnnn_n' Cuts at: 2574 3335 4676 2574 Size: 761 1341 2574 Fragments arranged by size: 2574 1341 761 BclI T'GATC_A Cuts at: 2196 2196 Size: 4676 BglII A'GATC_T Cuts at: 2191 4228 2191 Size: 2037 2639 BlpI GC'TnA_GC Cuts at: 1788 1788 Size: 4676 Bme1580I G_kGCm'C Cuts at: 968 980 2301 2394 3450 968 Size: 12 1321 93 1056 2194 Fragments arranged by size: 2194 1321 1056 93 12 BmrI ACTGGGnnnn_n' Cuts at: 355 709 1888 1969 2304 4448 355 Size: 354 1179 81 335 2144 583 Fragments arranged by size: 2144 1179 583 354 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3043 3043 Size: 4676 Bpu10I CC'TnA_GC Cuts at: 1241 1241 Size: 4676 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 542 1367 1852 2731 3223 3521 542 Size: 825 485 879 492 298 1697 Fragments arranged by size: 1697 879 825 492 485 298 BsaI GGTCTCn'nnnn_ Cuts at: 4380 4380 Size: 4676 BsaAI yAC'GTr Cuts at: 2029 2029 Size: 4676 BsaBI GATnn'nnATC Cuts at: 6 488 1583 2190 4227 6 Size: 482 1095 607 2037 455 Fragments arranged by size: 2037 1095 607 482 455 BsaHI Gr'CG_yC Cuts at: 2107 2355 3057 4553 2107 Size: 248 702 1496 2230 Fragments arranged by size: 2230 1496 702 248 BsaWI w'CCGG_w Cuts at: 1865 2386 3064 3338 3485 3896 1865 Size: 521 678 274 147 411 2645 Fragments arranged by size: 2645 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 121 2101 2859 3436 3932 121 Size: 1980 758 577 496 865 Fragments arranged by size: 1980 865 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 533 589 533 Size: 56 4620 BsiEI CG_ry'CG Cuts at: 104 2218 2264 3472 104 Size: 2114 46 1208 1308 Fragments arranged by size: 2114 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1690 2468 2658 3450 1690 Size: 778 190 792 2916 Fragments arranged by size: 2916 792 778 190 BsmI GAATG_Cn' Cuts at: 348 348 Size: 4676 Bsp1286I G_dGCh'C Cuts at: 903 968 980 1690 2301 2394 2468 2658 2720 Size: 65 12 710 611 93 74 190 62 Cuts at: 2720 3450 903 Size: 730 2129 Fragments arranged by size: 2129 730 710 611 190 93 74 65 62 12 BspEI T'CCGG_A Cuts at: 3896 3896 Size: 4676 BspHI T'CATG_A Cuts at: 3852 3852 Size: 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1726 1940 2242 2623 1726 Size: 214 302 381 3779 Fragments arranged by size: 3779 381 302 214 BsrBI CCG'CTC Cuts at: 50 2968 3022 4506 4671 50 Size: 2918 54 1484 165 55 Fragments arranged by size: 2918 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2588 2588 Size: 4676 BsrFI r'CCGG_y Cuts at: 1432 2674 2855 3064 1432 Size: 1242 181 209 3044 Fragments arranged by size: 3044 1242 209 181 BsrGI T'GTAC_A Cuts at: 1329 1329 Size: 4676 BssSI C'ACGA_G Cuts at: 2947 3305 2947 Size: 358 4318 BstAPI GCAn_nnn'nTGC Cuts at: 2053 2053 Size: 4676 BstBI TT'CG_AA Cuts at: 3037 3037 Size: 4676 BstYI r'GATC_y Cuts at: 1 801 1584 1701 2168 2191 2526 2772 3784 Size: 800 783 117 467 23 335 246 1012 Cuts at: 3784 3870 3882 4228 1 Size: 86 12 346 449 Fragments arranged by size: 1012 800 783 467 449 346 335 246 117 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4676 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 182 2626 2807 2970 182 Size: 2444 181 163 1888 Fragments arranged by size: 2444 1888 181 163 BtsI GCAGTG_nn' Cuts at: 1151 1919 1151 Size: 768 3908 DraI TTT'AAA Cuts at: 306 1675 306 Size: 1369 3307 DrdI GACnn_nn'nnGTC Cuts at: 2382 3240 2382 Size: 858 3818 EaeI y'GGCC_r Cuts at: 110 1430 2261 2435 2826 2853 4198 110 Size: 1320 831 174 391 27 1345 588 Fragments arranged by size: 1345 1320 831 588 391 174 27 EagI C'GGCC_G Cuts at: 2261 2261 Size: 4676 EarI CTCTTCn'nnn_ Cuts at: 722 2699 2909 722 Size: 1977 210 2489 Fragments arranged by size: 2489 1977 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2884 3194 3340 4486 2884 Size: 310 146 1146 3074 Fragments arranged by size: 3074 1146 310 146 EcoICRI GAG'CTC Cuts at: 1688 1688 Size: 4676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1622 2501 2933 3680 3948 1622 Size: 879 432 747 268 2350 Fragments arranged by size: 2350 879 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1815 1815 Size: 4676 EcoRI G'AATT_C Cuts at: 1680 1680 Size: 4676 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1266 2695 1266 Size: 1429 3247 FspI TGC'GCA Cuts at: 2457 2457 Size: 4676 HaeII r_GCGC'y Cuts at: 2110 2358 3380 4359 2110 Size: 248 1022 979 2427 Fragments arranged by size: 2427 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 674 706 1075 1107 1215 1247 2518 2548 2550 Size: 32 369 32 108 32 1271 30 2 Cuts at: 2550 2580 674 Size: 30 2770 Fragments arranged by size: 2770 1271 369 108 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1715 1715 Size: 4676 KasI G'GCGC_C Cuts at: 2106 2354 2106 Size: 248 4428 KpnI G_GTAC'C Cuts at: 1622 1696 1622 Size: 74 4602 MfeI C'AATT_G Cuts at: 370 1077 370 Size: 707 3969 MluI A'CGCG_T Cuts at: 4109 4109 Size: 4676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1184 1660 3347 3531 1184 Size: 476 1687 184 2329 Fragments arranged by size: 2329 1687 476 184 MscI TGG'CCA Cuts at: 2437 2437 Size: 4676 MslI CAynn'nnrTG Cuts at: 194 620 1514 2665 2792 194 Size: 426 894 1151 127 2078 Fragments arranged by size: 2078 1151 894 426 127 MspA1I CmG'CkG Cuts at: 1787 2101 2461 3474 3719 1787 Size: 314 360 1013 245 2744 Fragments arranged by size: 2744 1013 360 314 245 NaeI GCC'GGC Cuts at: 2857 2857 Size: 4676 NarI GG'CG_CC Cuts at: 2107 2355 2107 Size: 248 4428 NcoI C'CATG_G Cuts at: 107 107 Size: 4676 NgoMIV G'CCGG_C Cuts at: 2855 2855 Size: 4676 NspI r_CATG'y Cuts at: 1729 2229 3136 1729 Size: 500 907 3269 Fragments arranged by size: 3269 907 500 PciI A'CATG_T Cuts at: 3132 3132 Size: 4676 PmeI GTTT'AAAC Cuts at: 1675 1675 Size: 4676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 3815 Size: 32 4644 PstI C_TGCA'G Cuts at: 793 1723 2408 793 Size: 930 685 3061 Fragments arranged by size: 3061 930 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4676 PvuII CAG'CTG Cuts at: 2101 2461 2101 Size: 360 4316 RsrII CG'GwC_CG Cuts at: 2871 2871 Size: 4676 SacI G_AGCT'C Cuts at: 1690 1690 Size: 4676 SalI G'TCGA_C Cuts at: 1713 1713 Size: 4676 SapI GCTCTTCn'nnn_ Cuts at: 2699 2909 2699 Size: 210 4466 SbfI CC_TGCA'GG Cuts at: 1723 1723 Size: 4676 ScaI AGT'ACT Cuts at: 4193 4193 Size: 4676 SfcI C'TryA_G Cuts at: 33 789 1719 2404 3397 3588 33 Size: 756 930 685 993 191 1121 Fragments arranged by size: 1121 993 930 756 685 191 SfoI GGC'GCC Cuts at: 2108 2356 2108 Size: 248 4428 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4676 SmaI CCC'GGG Cuts at: 1698 1698 Size: 4676 SmlI C'TyrA_G Cuts at: 557 1382 1831 2746 3238 3500 557 Size: 825 449 915 492 262 1733 Fragments arranged by size: 1733 915 825 492 449 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4676 SphI G_CATG'C Cuts at: 1729 2229 1729 Size: 500 4176 StyI C'CwwG_G Cuts at: 107 1810 2164 107 Size: 1703 354 2619 Fragments arranged by size: 2619 1703 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1133 2259 2392 2851 3060 1133 Size: 1126 133 459 209 2749 Fragments arranged by size: 2749 1126 459 209 133 TatI w'GTAC_w Cuts at: 1329 4191 1329 Size: 2862 1814 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1132 1164 4193 4225 1132 Size: 32 3029 32 1583 Fragments arranged by size: 3029 1583 32 32 Tth111I GACn'n_nGTC Cuts at: 2473 2473 Size: 4676 XbaI T'CTAG_A Cuts at: 276 474 1401 1707 276 Size: 198 927 306 3245 Fragments arranged by size: 3245 927 306 198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2171 2171 Size: 4676 XmaI C'CCGG_G Cuts at: 1696 1696 Size: 4676 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI BseRI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI