(Circular) (Six-base) MAPSORT of: pF1KE2342.seq Check: 6213 from: 1 to: 4382 pF1KE2342 4382 bp With 182 enzymes: SgfI * March 29, 2011 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1171 1171 Size: 4382 AatII G_ACGT'C Cuts at: 719 719 Size: 4382 AccI GT'mk_AC Cuts at: 926 1127 1420 926 Size: 201 293 3888 Fragments arranged by size: 3888 293 201 Acc65I G'GTAC_C Cuts at: 1398 1398 Size: 4382 AclI AA'CG_TT Cuts at: 4222 4222 Size: 4382 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 262 2207 2639 3386 3654 262 Size: 1945 432 747 268 990 Fragments arranged by size: 1945 990 747 432 268 AflIII A'CryG_T Cuts at: 1091 1734 2838 3815 1091 Size: 643 1104 977 1658 Fragments arranged by size: 1658 1104 977 643 AgeI A'CCGG_T Cuts at: 2770 2770 Size: 4382 AlwNI CAG_nnn'CTG Cuts at: 391 619 1687 3254 391 Size: 228 1068 1567 1519 Fragments arranged by size: 1567 1519 1068 228 ApaI G_GGCC'C Cuts at: 195 195 Size: 4382 ApaLI G'TGCA_C Cuts at: 3152 3152 Size: 4382 ApoI r'AATT_y Cuts at: 343 1386 3735 3991 343 Size: 1043 2349 256 734 Fragments arranged by size: 2349 1043 734 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4382 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4382 AvaI C'yCGr_G Cuts at: 455 764 1402 455 Size: 309 638 3435 Fragments arranged by size: 3435 638 309 BamHI G'GATC_C Cuts at: 1407 1407 Size: 4382 BanI G'GyrC_C Cuts at: 487 1398 1812 2060 2095 487 Size: 911 414 248 35 2774 Fragments arranged by size: 2774 911 414 248 35 BanII G_rGCy'C Cuts at: 195 479 591 608 850 989 1396 2426 195 Size: 284 112 17 242 139 407 1030 2151 Fragments arranged by size: 2151 1030 407 284 242 139 112 17 BbeI G_GCGC'C Cuts at: 1816 2064 1816 Size: 248 4134 BbsI GAAGACnn'nnnn_ Cuts at: 881 881 Size: 4382 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 108 142 108 Size: 34 4348 BciVI GTATCCnnnnn_n' Cuts at: 2280 3041 4382 2280 Size: 761 1341 2280 Fragments arranged by size: 2280 1341 761 BclI T'GATC_A Cuts at: 566 1902 566 Size: 1336 3046 BglI GCCn_nnn'nGGC Cuts at: 298 984 298 Size: 686 3696 BglII A'GATC_T Cuts at: 741 1897 3934 741 Size: 1156 2037 1189 Fragments arranged by size: 2037 1189 1156 BlpI GC'TnA_GC Cuts at: 232 1494 232 Size: 1262 3120 Bme1580I G_kGCm'C Cuts at: 195 492 2007 2100 3156 195 Size: 297 1515 93 1056 1421 Fragments arranged by size: 1515 1421 1056 297 93 BmrI ACTGGGnnnn_n' Cuts at: 1594 1675 2010 4154 1594 Size: 81 335 2144 1822 Fragments arranged by size: 2144 1822 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2749 2749 Size: 4382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 171 445 973 171 Size: 274 528 3580 Fragments arranged by size: 3580 528 274 Bpu10I CC'TnA_GC Cuts at: 1169 1169 Size: 4382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1558 2437 2929 3227 1558 Size: 879 492 298 2713 Fragments arranged by size: 2713 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4086 4086 Size: 4382 BsaAI yAC'GTr Cuts at: 930 1029 1735 930 Size: 99 706 3577 Fragments arranged by size: 3577 706 99 BsaBI GATnn'nnATC Cuts at: 6 1896 3933 6 Size: 1890 2037 455 Fragments arranged by size: 2037 1890 455 BsaHI Gr'CG_yC Cuts at: 716 1813 2061 2763 4259 716 Size: 1097 248 702 1496 839 Fragments arranged by size: 1496 1097 839 702 248 BsaWI w'CCGG_w Cuts at: 463 1571 2092 2770 3044 3191 3602 463 Size: 1108 521 678 274 147 411 1243 Fragments arranged by size: 1243 1108 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 253 460 253 Size: 207 4175 BseYI C'CCAG_C Cuts at: 129 185 357 834 1318 1807 2565 3142 3638 Size: 56 172 477 484 489 758 577 496 Cuts at: 3638 129 Size: 873 Fragments arranged by size: 873 758 577 496 489 484 477 172 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1009 1199 1009 Size: 190 4192 BsiEI CG_ry'CG Cuts at: 104 163 315 1924 1970 3178 104 Size: 59 152 1609 46 1208 1308 Fragments arranged by size: 1609 1308 1208 152 59 46 BsiHKAI G_wGCw'C Cuts at: 287 1396 2174 2364 3156 287 Size: 1109 778 190 792 1513 Fragments arranged by size: 1513 1109 792 778 190 BsmI GAATG_Cn' Cuts at: 1232 1232 Size: 4382 Bsp1286I G_dGCh'C Cuts at: 195 287 479 492 591 608 850 989 1396 Size: 92 192 13 99 17 242 139 407 Cuts at: 1396 2007 2100 2174 2364 2426 3156 195 Size: 611 93 74 190 62 730 1421 Fragments arranged by size: 1421 730 611 407 242 192 190 139 99 93 92 74 62 17 13 BspEI T'CCGG_A Cuts at: 463 3602 463 Size: 3139 1243 BspHI T'CATG_A Cuts at: 3558 3558 Size: 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 344 1031 1171 1432 1646 1948 2329 344 Size: 687 140 261 214 302 381 2397 Fragments arranged by size: 2397 687 381 302 261 214 140 BsrBI CCG'CTC Cuts at: 50 957 2674 2728 4212 4377 50 Size: 907 1717 54 1484 165 55 Fragments arranged by size: 1717 1484 907 165 55 54 BsrDI GCAATG_nn' Cuts at: 327 531 1158 1257 2294 327 Size: 204 627 99 1037 2415 Fragments arranged by size: 2415 1037 627 204 99 BsrFI r'CCGG_y Cuts at: 172 2380 2561 2770 172 Size: 2208 181 209 1784 Fragments arranged by size: 2208 1784 209 181 BsrGI T'GTAC_A Cuts at: 1094 1094 Size: 4382 BssSI C'ACGA_G Cuts at: 2653 3011 2653 Size: 358 4024 BstAPI GCAn_nnn'nTGC Cuts at: 1759 1759 Size: 4382 BstBI TT'CG_AA Cuts at: 2743 2743 Size: 4382 BstXI CCAn_nnnn'nTGG Cuts at: 903 903 Size: 4382 BstYI r'GATC_y Cuts at: 1 741 1407 1874 1897 2232 2478 3490 3576 Size: 740 666 467 23 335 246 1012 86 Cuts at: 3576 3588 3934 1 Size: 12 346 449 Fragments arranged by size: 1012 740 666 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 209 263 1274 107 Size: 102 54 1011 3215 Fragments arranged by size: 3215 1011 102 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1163 2332 2513 2676 1163 Size: 1169 181 163 2869 Fragments arranged by size: 2869 1169 181 163 BtsI GCAGTG_nn' Cuts at: 476 1163 1625 476 Size: 687 462 3233 Fragments arranged by size: 3233 687 462 ClaI AT'CG_AT Cuts at: 311 311 Size: 4382 DraI TTT'AAA Cuts at: 1381 1381 Size: 4382 DrdI GACnn_nn'nnGTC Cuts at: 2088 2946 2088 Size: 858 3524 EaeI y'GGCC_r Cuts at: 160 593 968 1044 1056 1967 2141 2532 2559 Size: 433 375 76 12 911 174 391 27 Cuts at: 2559 3904 160 Size: 1345 638 Fragments arranged by size: 1345 911 638 433 391 375 174 76 27 12 EagI C'GGCC_G Cuts at: 160 1967 160 Size: 1807 2575 EarI CTCTTCn'nnn_ Cuts at: 2405 2615 2405 Size: 210 4172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2590 2900 3046 4192 2590 Size: 310 146 1146 2780 Fragments arranged by size: 2780 1146 310 146 EcoICRI GAG'CTC Cuts at: 1394 1394 Size: 4382 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 171 262 445 973 2207 2639 3386 3654 171 Size: 91 183 528 1234 432 747 268 899 Fragments arranged by size: 1234 899 747 528 432 268 183 91 EcoO109I rG'GnC_Cy Cuts at: 976 1521 976 Size: 545 3837 EcoRI G'AATT_C Cuts at: 1386 1386 Size: 4382 EcoRV GAT'ATC Cuts at: 452 452 Size: 4382 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 4382 FspI TGC'GCA Cuts at: 2163 2163 Size: 4382 HaeII r_GCGC'y Cuts at: 1816 2064 3086 4065 1816 Size: 248 1022 979 2133 Fragments arranged by size: 2133 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 105 137 436 468 613 645 2224 2254 2256 Size: 32 299 32 145 32 1579 30 2 Cuts at: 2256 2286 105 Size: 30 2201 Fragments arranged by size: 2201 1579 299 145 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1421 1421 Size: 4382 KasI G'GCGC_C Cuts at: 1812 2060 1812 Size: 248 4134 KpnI G_GTAC'C Cuts at: 1402 1402 Size: 4382 MluI A'CGCG_T Cuts at: 3815 3815 Size: 4382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 521 3053 3237 521 Size: 2532 184 1666 Fragments arranged by size: 2532 1666 184 MscI TGG'CCA Cuts at: 595 1046 2143 595 Size: 451 1097 2834 Fragments arranged by size: 2834 1097 451 MslI CAynn'nnrTG Cuts at: 2371 2498 2371 Size: 127 4255 MspA1I CmG'CkG Cuts at: 189 357 1201 1493 1807 2167 3180 3425 189 Size: 168 844 292 314 360 1013 245 1146 Fragments arranged by size: 1146 1013 844 360 314 292 245 168 NaeI GCC'GGC Cuts at: 174 2563 174 Size: 2389 1993 NarI GG'CG_CC Cuts at: 1813 2061 1813 Size: 248 4134 NcoI C'CATG_G Cuts at: 107 263 1274 107 Size: 156 1011 3215 Fragments arranged by size: 3215 1011 156 NgoMIV G'CCGG_C Cuts at: 172 2561 172 Size: 2389 1993 NotI GC'GGCC_GC Cuts at: 160 160 Size: 4382 NspI r_CATG'y Cuts at: 433 1095 1435 1935 2842 433 Size: 662 340 500 907 1973 Fragments arranged by size: 1973 907 662 500 340 PciI A'CATG_T Cuts at: 1091 2838 1091 Size: 1747 2635 PfoI T'CCnGG_A Cuts at: 1001 1001 Size: 4382 PmeI GTTT'AAAC Cuts at: 1381 1381 Size: 4382 PmlI CAC'GTG Cuts at: 1029 1029 Size: 4382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3521 3553 3521 Size: 32 4350 PspOMI G'GGCC_C Cuts at: 191 191 Size: 4382 PstI C_TGCA'G Cuts at: 1429 2114 1429 Size: 685 3697 PvuI CG_AT'CG Cuts at: 104 315 104 Size: 211 4171 PvuII CAG'CTG Cuts at: 357 1201 1807 2167 357 Size: 844 606 360 2572 Fragments arranged by size: 2572 844 606 360 RsrII CG'GwC_CG Cuts at: 2577 2577 Size: 4382 SacI G_AGCT'C Cuts at: 1396 1396 Size: 4382 SalI G'TCGA_C Cuts at: 1419 1419 Size: 4382 SapI GCTCTTCn'nnn_ Cuts at: 2405 2615 2405 Size: 210 4172 SbfI CC_TGCA'GG Cuts at: 1429 1429 Size: 4382 ScaI AGT'ACT Cuts at: 3899 3899 Size: 4382 SfcI C'TryA_G Cuts at: 33 1425 2110 3103 3294 33 Size: 1392 685 993 191 1121 Fragments arranged by size: 1392 1121 993 685 191 SfiI GGCCn_nnn'nGGCC Cuts at: 298 298 Size: 4382 SfoI GGC'GCC Cuts at: 1814 2062 1814 Size: 248 4134 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4382 SmaI CCC'GGG Cuts at: 1404 1404 Size: 4382 SmlI C'TyrA_G Cuts at: 1537 2452 2944 3206 1537 Size: 915 492 262 2713 Fragments arranged by size: 2713 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4382 SphI G_CATG'C Cuts at: 1435 1935 1435 Size: 500 3882 StuI AGG'CCT Cuts at: 1144 1144 Size: 4382 StyI C'CwwG_G Cuts at: 107 263 296 1139 1274 1516 1870 107 Size: 156 33 843 135 242 354 2619 Fragments arranged by size: 2619 843 354 242 156 135 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1965 2098 2557 2766 1965 Size: 133 459 209 3581 Fragments arranged by size: 3581 459 209 133 TatI w'GTAC_w Cuts at: 578 1094 3897 578 Size: 516 2803 1063 Fragments arranged by size: 2803 1063 516 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3899 3931 3899 Size: 32 4350 Tth111I GACn'n_nGTC Cuts at: 2179 2179 Size: 4382 XbaI T'CTAG_A Cuts at: 1413 1413 Size: 4382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 741 1877 741 Size: 1136 3246 XmaI C'CCGG_G Cuts at: 1402 1402 Size: 4382 ZraI GAC'GTC Cuts at: 717 717 Size: 4382 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PasI PflMI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI