Restriction map for
pF1KB6195
(Circular) (Six-base) MAPSORT of: pF1KB6195.seq Check: 5807 from: 1 to: 5102
pF1KB6195 5102 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 2140 2140
Size: 5102
Acc65I G'GTAC_C
Cuts at: 225 2118 225
Size: 1893 3209
AclI AA'CG_TT
Cuts at: 4942 4942
Size: 5102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 1286 1814 1819 2927 3359 4106 4374 432
Size: 854 528 5 1108 432 747 268 1160
Fragments arranged by size:
1160 1108 854 747 528 432 268 5
AflIII A'CryG_T
Cuts at: 2454 3558 4535 2454
Size: 1104 977 3021
Fragments arranged by size:
3021 1104 977
AgeI A'CCGG_T
Cuts at: 3490 3490
Size: 5102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 5102
AlwNI CAG_nnn'CTG
Cuts at: 330 963 1627 1945 2407 3974 330
Size: 633 664 318 462 1567 1458
Fragments arranged by size:
1567 1458 664 633 462 318
ApaI G_GGCC'C
Cuts at: 1643 1770 1643
Size: 127 4975
ApaLI G'TGCA_C
Cuts at: 3872 3872
Size: 5102
ApoI r'AATT_y
Cuts at: 1163 2106 4455 4711 1163
Size: 943 2349 256 1554
Fragments arranged by size:
2349 1554 943 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5102
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
AvaI C'yCGr_G
Cuts at: 255 2122 255
Size: 1867 3235
AvrII C'CTAG_G
Cuts at: 1323 1708 1323
Size: 385 4717
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 648 681 648
Size: 33 5069
BamHI G'GATC_C
Cuts at: 2127 2127
Size: 5102
BanI G'GyrC_C
Cuts at: 225 1605 2118 2532 2780 2815 225
Size: 1380 513 414 248 35 2512
Fragments arranged by size:
2512 1380 513 414 248 35
BanII G_rGCy'C
Cuts at: 530 1351 1545 1643 1770 1814 1958 2087 2116
Size: 821 194 98 127 44 144 129 29
Cuts at: 2116 3146 530
Size: 1030 2486
Fragments arranged by size:
2486 1030 821 194 144 129 127 98
44 29
BbeI G_GCGC'C
Cuts at: 1609 2536 2784 1609
Size: 927 248 3927
Fragments arranged by size:
3927 927 248
BbsI GAAGACnn'nnnn_
Cuts at: 194 622 652 194
Size: 428 30 4644
Fragments arranged by size:
4644 428 30
BciVI GTATCCnnnnn_n'
Cuts at: 3000 3761 5102 3000
Size: 761 1341 3000
Fragments arranged by size:
3000 1341 761
BclI T'GATC_A
Cuts at: 2622 2622
Size: 5102
BglI GCCn_nnn'nGGC
Cuts at: 1060 1069 1540 1889 1060
Size: 9 471 349 4273
Fragments arranged by size:
4273 471 349 9
BglII A'GATC_T
Cuts at: 443 1117 2617 4654 443
Size: 674 1500 2037 891
Fragments arranged by size:
2037 1500 891 674
BlpI GC'TnA_GC
Cuts at: 1988 2214 1988
Size: 226 4876
Bme1580I G_kGCm'C
Cuts at: 912 1310 1643 1770 2727 2820 3876 912
Size: 398 333 127 957 93 1056 2138
Fragments arranged by size:
2138 1056 957 398 333 127 93
BmrI ACTGGGnnnn_n'
Cuts at: 122 629 1933 2314 2395 2730 4874 122
Size: 507 1304 381 81 335 2144 350
Fragments arranged by size:
2144 1304 507 381 350 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 387 609 1977 3469 387
Size: 222 1368 1492 2020
Fragments arranged by size:
2020 1492 1368 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 579 601 1086 1165 1241 1423 283
Size: 296 22 485 79 76 182 3962
Fragments arranged by size:
3962 485 296 182 79 76 22
Bpu10I CC'TnA_GC
Cuts at: 1968 1968
Size: 5102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1972 2278 3157 3649 3947 1972
Size: 306 879 492 298 3127
Fragments arranged by size:
3127 879 492 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 1113 1183 1792 4806 1113
Size: 70 609 3014 1409
Fragments arranged by size:
3014 1409 609 70
BsaAI yAC'GTr
Cuts at: 2455 2455
Size: 5102
BsaBI GATnn'nnATC
Cuts at: 6 2616 4653 6
Size: 2610 2037 455
Fragments arranged by size:
2610 2037 455
BsaHI Gr'CG_yC
Cuts at: 1606 2533 2781 3483 4979 1606
Size: 927 248 702 1496 1729
Fragments arranged by size:
1729 1496 927 702 248
BsaWI w'CCGG_w
Cuts at: 641 2291 2812 3490 3764 3911 4322 641
Size: 1650 521 678 274 147 411 1421
Fragments arranged by size:
1650 1421 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1404 1434 1890 1920 1404
Size: 30 456 30 4586
Fragments arranged by size:
4586 456 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 1237 1728 1898 1950 598
Size: 639 491 170 52 3750
Fragments arranged by size:
3750 639 491 170 52
BseYI C'CCAG_C
Cuts at: 204 418 685 803 1309 1579 1642 1912 2037
Size: 214 267 118 506 270 63 270 125
Cuts at: 2037 2527 3285 3862 4358 204
Size: 490 758 577 496 948
Fragments arranged by size:
948 758 577 506 496 490 270 270
267 214 125 118 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 467
Size: 5102
BsiEI CG_ry'CG
Cuts at: 104 2644 2690 3898 104
Size: 2540 46 1208 1308
Fragments arranged by size:
2540 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1814 1958 2116 2894 3084 3876 1814
Size: 144 158 778 190 792 3040
Fragments arranged by size:
3040 792 778 190 158 144
BsmI GAATG_Cn'
Cuts at: 573 573
Size: 5102
BsmBI CGTCTCn'nnnn_
Cuts at: 1257 1257
Size: 5102
Bsp1286I G_dGCh'C
Cuts at: 530 912 1310 1351 1545 1643 1770 1814 1958
Size: 382 398 41 194 98 127 44 144
Cuts at: 1958 2087 2116 2727 2820 2894 3084 3146 3876
Size: 129 29 611 93 74 190 62 730
Cuts at: 3876 530
Size: 1756
Fragments arranged by size:
1756 730 611 398 382 194 190 144
129 127 98 93 74 62 44 41
29
BspEI T'CCGG_A
Cuts at: 641 4322 641
Size: 3681 1421
BspHI T'CATG_A
Cuts at: 4278 4278
Size: 5102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 213 2152 2366 2668 3049 213
Size: 1939 214 302 381 2266
Fragments arranged by size:
2266 1939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3394 3448 4932 5097 50
Size: 3344 54 1484 165 55
Fragments arranged by size:
3344 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 3014 1667
Size: 1347 3755
BsrFI r'CCGG_y
Cuts at: 3100 3281 3490 3100
Size: 181 209 4712
Fragments arranged by size:
4712 209 181
BssSI C'ACGA_G
Cuts at: 3373 3731 3373
Size: 358 4744
BstAPI GCAn_nnn'nTGC
Cuts at: 2479 2479
Size: 5102
BstBI TT'CG_AA
Cuts at: 3463 3463
Size: 5102
BstXI CCAn_nnnn'nTGG
Cuts at: 996 996
Size: 5102
BstYI r'GATC_y
Cuts at: 1 326 443 1117 1226 2026 2127 2594 2617
Size: 325 117 674 109 800 101 467 23
Cuts at: 2617 2952 3198 4210 4296 4308 4654 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 800 674 467 449 346 335 325
246 117 109 101 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1049 1804 1962 1049
Size: 755 158 4189
Fragments arranged by size:
4189 755 158
BtgI C'CryG_G
Cuts at: 107 389 1058 1337 1382 1613 1772 107
Size: 282 669 279 45 231 159 3437
Fragments arranged by size:
3437 669 282 279 231 159 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3052 3233 3396 3052
Size: 181 163 4758
Fragments arranged by size:
4758 181 163
BtsI GCAGTG_nn'
Cuts at: 2345 2345
Size: 5102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1835 1870 1835
Size: 35 5067
DraI TTT'AAA
Cuts at: 2101 2101
Size: 5102
DrdI GACnn_nn'nnGTC
Cuts at: 1000 2808 3666 1000
Size: 1808 858 2436
Fragments arranged by size:
2436 1808 858
EaeI y'GGCC_r
Cuts at: 941 2687 2861 3252 3279 4624 941
Size: 1746 174 391 27 1345 1419
Fragments arranged by size:
1746 1419 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2687 2687
Size: 5102
EarI CTCTTCn'nnn_
Cuts at: 628 764 1149 1790 3125 3335 628
Size: 136 385 641 1335 210 2395
Fragments arranged by size:
2395 1335 641 385 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3310 3620 3766 4912 3310
Size: 310 146 1146 3500
Fragments arranged by size:
3500 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1812 1956 2114 1812
Size: 144 158 4800
Fragments arranged by size:
4800 158 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 432 579 601 1086 1165 1241 1286 1423
Size: 149 147 22 485 79 76 45 137
Cuts at: 1423 1814 1819 2927 3359 4106 4374 283
Size: 391 5 1108 432 747 268 1011
Fragments arranged by size:
1108 1011 747 485 432 391 268 149
147 137 79 76 45 22 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1680 1680
Size: 5102
EcoO109I rG'GnC_Cy
Cuts at: 302 993 1639 1733 1766 1767 1973 2241 302
Size: 691 646 94 33 1 206 268 3163
Fragments arranged by size:
3163 691 646 268 206 94 33 1
EcoRI G'AATT_C
Cuts at: 2106 2106
Size: 5102
EcoRV GAT'ATC
Cuts at: 174 426 174
Size: 252 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 462 1491 3121 462
Size: 1029 1630 2443
Fragments arranged by size:
2443 1630 1029
FspI TGC'GCA
Cuts at: 2883 2883
Size: 5102
HaeII r_GCGC'y
Cuts at: 1609 2536 2784 3806 4785 1609
Size: 927 248 1022 979 1926
Fragments arranged by size:
1926 1022 979 927 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 306 338 1890 1922 2944 2974 2976 3006 306
Size: 32 1552 32 1022 30 2 30 2402
Fragments arranged by size:
2402 1552 1022 32 32 30 30 2
HincII GTy'rAC
Cuts at: 386 2141 386
Size: 1755 3347
KasI G'GCGC_C
Cuts at: 1605 2532 2780 1605
Size: 927 248 3927
Fragments arranged by size:
3927 927 248
KpnI G_GTAC'C
Cuts at: 229 2122 229
Size: 1893 3209
MluI A'CGCG_T
Cuts at: 4535 4535
Size: 5102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 1618 1870 3773 3957 248
Size: 1370 252 1903 184 1393
Fragments arranged by size:
1903 1393 1370 252 184
MscI TGG'CCA
Cuts at: 943 2863 943
Size: 1920 3182
MslI CAynn'nnrTG
Cuts at: 2077 3091 3218 2077
Size: 1014 127 3961
Fragments arranged by size:
3961 1014 127
MspA1I CmG'CkG
Cuts at: 183 208 391 490 1453 2213 2527 2887 3900
Size: 25 183 99 963 760 314 360 1013
Cuts at: 3900 4145 183
Size: 245 1140
Fragments arranged by size:
1140 1013 963 760 360 314 245 183
99 25
NaeI GCC'GGC
Cuts at: 3283 3283
Size: 5102
NarI GG'CG_CC
Cuts at: 1606 2533 2781 1606
Size: 927 248 3927
Fragments arranged by size:
3927 927 248
NcoI C'CATG_G
Cuts at: 107 1772 107
Size: 1665 3437
NgoMIV G'CCGG_C
Cuts at: 3281 3281
Size: 5102
NspI r_CATG'y
Cuts at: 1174 2155 2655 3562 1174
Size: 981 500 907 2714
Fragments arranged by size:
2714 981 907 500
PasI CC'CwG_GG
Cuts at: 1128 1537 1657 1128
Size: 409 120 4573
Fragments arranged by size:
4573 409 120
PciI A'CATG_T
Cuts at: 3558 3558
Size: 5102
PflMI CCAn_nnn'nTGG
Cuts at: 183 857 1575 183
Size: 674 718 3710
Fragments arranged by size:
3710 718 674
PfoI T'CCnGG_A
Cuts at: 329 428 329
Size: 99 5003
PmeI GTTT'AAAC
Cuts at: 2101 2101
Size: 5102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1940 1972 4241 4273 1940
Size: 32 2269 32 2769
Fragments arranged by size:
2769 2269 32 32
PpuMI rG'GwC_Cy
Cuts at: 302 993 1733 1973 302
Size: 691 740 240 3431
Fragments arranged by size:
3431 740 691 240
PspOMI G'GGCC_C
Cuts at: 1639 1766 1639
Size: 127 4975
PstI C_TGCA'G
Cuts at: 1367 2149 2834 1367
Size: 782 685 3635
Fragments arranged by size:
3635 782 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5102
PvuII CAG'CTG
Cuts at: 183 208 490 2527 2887 183
Size: 25 282 2037 360 2398
Fragments arranged by size:
2398 2037 360 282 25
RsrII CG'GwC_CG
Cuts at: 3297 3297
Size: 5102
SacI G_AGCT'C
Cuts at: 1814 1958 2116 1814
Size: 144 158 4800
Fragments arranged by size:
4800 158 144
SacII CC_GC'GG
Cuts at: 392 392
Size: 5102
SalI G'TCGA_C
Cuts at: 2139 2139
Size: 5102
SanDI GG'GwC_CC
Cuts at: 1973 1973
Size: 5102
SapI GCTCTTCn'nnn_
Cuts at: 764 1149 3125 3335 764
Size: 385 1976 210 2531
Fragments arranged by size:
2531 1976 385 210
SbfI CC_TGCA'GG
Cuts at: 2149 2149
Size: 5102
ScaI AGT'ACT
Cuts at: 1413 4619 1413
Size: 3206 1896
SexAI A'CCwGG_T
Cuts at: 995 995
Size: 5102
SfcI C'TryA_G
Cuts at: 33 121 1363 1697 2145 2830 3823 4014 33
Size: 88 1242 334 448 685 993 191 1121
Fragments arranged by size:
1242 1121 993 685 448 334 191 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 1060 1069 1060
Size: 9 5093
SfoI GGC'GCC
Cuts at: 1607 2534 2782 1607
Size: 927 248 3927
Fragments arranged by size:
3927 927 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
SmaI CCC'GGG
Cuts at: 2124 2124
Size: 5102
SmlI C'TyrA_G
Cuts at: 1951 2257 3172 3664 3926 1951
Size: 306 915 492 262 3127
Fragments arranged by size:
3127 915 492 306 262
SpeI A'CTAG_T
Cuts at: 67 556 67
Size: 489 4613
SphI G_CATG'C
Cuts at: 2155 2655 2155
Size: 500 4602
StuI AGG'CCT
Cuts at: 592 1054 1489 592
Size: 462 435 4205
Fragments arranged by size:
4205 462 435
StyI C'CwwG_G
Cuts at: 107 650 989 1323 1708 1772 2236 2590 107
Size: 543 339 334 385 64 464 354 2619
Fragments arranged by size:
2619 543 464 385 354 339 334 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1165 1626 2685 2818 3277 3486 1165
Size: 461 1059 133 459 209 2781
Fragments arranged by size:
2781 1059 461 459 209 133
TatI w'GTAC_w
Cuts at: 251 788 1411 4617 251
Size: 537 623 3206 736
Fragments arranged by size:
3206 736 623 537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4619 4651 4619
Size: 32 5070
Tth111I GACn'n_nGTC
Cuts at: 1902 2899 1902
Size: 997 4105
XbaI T'CTAG_A
Cuts at: 2133 2133
Size: 5102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 996 1065 1576 2597 426
Size: 570 69 511 1021 2931
Fragments arranged by size:
2931 1021 570 511 69
XmaI C'CCGG_G
Cuts at: 2122 2122
Size: 5102
XmnI GAAnn'nnTTC
Cuts at: 464 464
Size: 5102
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I ClaI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI