Restriction map for
pF1KB6195
(Linear) (Six-base) MAPSORT of: KIBB6195.seq Check: 8206 from: 1 to: 1989
KIBB6195.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
Acc65I G'GTAC_C
Cuts at: 0 117 1989
Size: 117 1872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1178 1706 1711 1989
Size: 324 854 528 5 278
Fragments arranged by size:
854 528 324 278 5
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 1989
Size: 253 1736
AlwNI CAG_nnn'CTG
Cuts at: 0 222 855 1519 1837 1989
Size: 222 633 664 318 152
Fragments arranged by size:
664 633 318 222 152
ApaI G_GGCC'C
Cuts at: 0 1535 1662 1989
Size: 1535 127 327
Fragments arranged by size:
1535 327 127
ApoI r'AATT_y
Cuts at: 0 1055 1989
Size: 1055 934
AvaI C'yCGr_G
Cuts at: 0 147 1989
Size: 147 1842
AvrII C'CTAG_G
Cuts at: 0 1215 1600 1989
Size: 1215 385 389
Fragments arranged by size:
1215 389 385
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 540 573 1989
Size: 540 33 1416
Fragments arranged by size:
1416 540 33
BanI G'GyrC_C
Cuts at: 0 117 1497 1989
Size: 117 1380 492
Fragments arranged by size:
1380 492 117
BanII G_rGCy'C
Cuts at: 0 422 1243 1437 1535 1662 1706 1850 1979
Size: 422 821 194 98 127 44 144 129
Cuts at: 1979 1989
Size: 10
Fragments arranged by size:
821 422 194 144 129 127 98 44
10
BbeI G_GCGC'C
Cuts at: 0 1501 1989
Size: 1501 488
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 514 544 1989
Size: 86 428 30 1445
Fragments arranged by size:
1445 428 86 30
BglI GCCn_nnn'nGGC
Cuts at: 0 952 961 1432 1781 1989
Size: 952 9 471 349 208
Fragments arranged by size:
952 471 349 208 9
BglII A'GATC_T
Cuts at: 0 335 1009 1989
Size: 335 674 980
Fragments arranged by size:
980 674 335
BlpI GC'TnA_GC
Cuts at: 0 1880 1989
Size: 1880 109
Bme1580I G_kGCm'C
Cuts at: 0 804 1202 1535 1662 1989
Size: 804 398 333 127 327
Fragments arranged by size:
804 398 333 327 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 521 1825 1989
Size: 14 507 1304 164
Fragments arranged by size:
1304 507 164 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 279 501 1869 1989
Size: 279 222 1368 120
Fragments arranged by size:
1368 279 222 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 471 493 978 1057 1133 1315 1989
Size: 175 296 22 485 79 76 182 674
Fragments arranged by size:
674 485 296 182 175 79 76 22
Bpu10I CC'TnA_GC
Cuts at: 0 1860 1989
Size: 1860 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1864 1989
Size: 1864 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 1005 1075 1684 1989
Size: 1005 70 609 305
Fragments arranged by size:
1005 609 305 70
BsaHI Gr'CG_yC
Cuts at: 0 1498 1989
Size: 1498 491
BsaWI w'CCGG_w
Cuts at: 0 533 1989
Size: 533 1456
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1296 1326 1782 1812 1989
Size: 1296 30 456 30 177
Fragments arranged by size:
1296 456 177 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 1129 1620 1790 1842 1989
Size: 490 639 491 170 52 147
Fragments arranged by size:
639 491 490 170 147 52
BseYI C'CCAG_C
Cuts at: 0 96 310 577 695 1201 1471 1534 1804
Size: 96 214 267 118 506 270 63 270
Cuts at: 1804 1929 1989
Size: 125 60
Fragments arranged by size:
506 270 270 267 214 125 118 96
63 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 1989
Size: 359 1630
BsiHKAI G_wGCw'C
Cuts at: 0 1706 1850 1989
Size: 1706 144 139
Fragments arranged by size:
1706 144 139
BsmI GAATG_Cn'
Cuts at: 0 465 1989
Size: 465 1524
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1149 1989
Size: 1149 840
Bsp1286I G_dGCh'C
Cuts at: 0 422 804 1202 1243 1437 1535 1662 1706
Size: 422 382 398 41 194 98 127 44
Cuts at: 1706 1850 1979 1989
Size: 144 129 10
Fragments arranged by size:
422 398 382 194 144 129 127 98
44 41 10
BspEI T'CCGG_A
Cuts at: 0 533 1989
Size: 533 1456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 1989
Size: 105 1884
BsrDI GCAATG_nn'
Cuts at: 0 1559 1989
Size: 1559 430
BstXI CCAn_nnnn'nTGG
Cuts at: 0 888 1989
Size: 888 1101
BstYI r'GATC_y
Cuts at: 0 218 335 1009 1118 1918 1989
Size: 218 117 674 109 800 71
Fragments arranged by size:
800 674 218 117 109 71
Bsu36I CC'TnA_GG
Cuts at: 0 941 1696 1854 1989
Size: 941 755 158 135
Fragments arranged by size:
941 755 158 135
BtgI C'CryG_G
Cuts at: 0 281 950 1229 1274 1505 1664 1989
Size: 281 669 279 45 231 159 325
Fragments arranged by size:
669 325 281 279 231 159 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1727 1762 1989
Size: 1727 35 227
Fragments arranged by size:
1727 227 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 892 1989
Size: 892 1097
EaeI y'GGCC_r
Cuts at: 0 833 1989
Size: 833 1156
EarI CTCTTCn'nnn_
Cuts at: 0 520 656 1041 1682 1989
Size: 520 136 385 641 307
Fragments arranged by size:
641 520 385 307 136
EcoICRI GAG'CTC
Cuts at: 0 1704 1848 1989
Size: 1704 144 141
Fragments arranged by size:
1704 144 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 324 471 493 978 1057 1133 1178
Size: 175 149 147 22 485 79 76 45
Cuts at: 1178 1315 1706 1711 1989
Size: 137 391 5 278
Fragments arranged by size:
485 391 278 175 149 147 137 79
76 45 22 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1572 1989
Size: 1572 417
EcoO109I rG'GnC_Cy
Cuts at: 0 194 885 1531 1625 1658 1659 1865 1989
Size: 194 691 646 94 33 1 206 124
Fragments arranged by size:
691 646 206 194 124 94 33 1
EcoRV GAT'ATC
Cuts at: 0 66 318 1989
Size: 66 252 1671
Fragments arranged by size:
1671 252 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 354 1383 1989
Size: 354 1029 606
Fragments arranged by size:
1029 606 354
HaeII r_GCGC'y
Cuts at: 0 1501 1989
Size: 1501 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 198 230 1782 1814 1989
Size: 198 32 1552 32 175
Fragments arranged by size:
1552 198 175 32 32
HincII GTy'rAC
Cuts at: 0 278 1989
Size: 278 1711
KasI G'GCGC_C
Cuts at: 0 1497 1989
Size: 1497 492
KpnI G_GTAC'C
Cuts at: 0 121 1989
Size: 121 1868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1510 1762 1989
Size: 140 1370 252 227
Fragments arranged by size:
1370 252 227 140
MscI TGG'CCA
Cuts at: 0 835 1989
Size: 835 1154
MslI CAynn'nnrTG
Cuts at: 0 1969 1989
Size: 1969 20
MspA1I CmG'CkG
Cuts at: 0 75 100 283 382 1345 1989
Size: 75 25 183 99 963 644
Fragments arranged by size:
963 644 183 99 75 25
NarI GG'CG_CC
Cuts at: 0 1498 1989
Size: 1498 491
NcoI C'CATG_G
Cuts at: 0 1664 1989
Size: 1664 325
NspI r_CATG'y
Cuts at: 0 1066 1989
Size: 1066 923
PasI CC'CwG_GG
Cuts at: 0 1020 1429 1549 1989
Size: 1020 409 120 440
Fragments arranged by size:
1020 440 409 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 749 1467 1989
Size: 75 674 718 522
Fragments arranged by size:
718 674 522 75
PfoI T'CCnGG_A
Cuts at: 0 221 320 1989
Size: 221 99 1669
Fragments arranged by size:
1669 221 99
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1832 1864 1989
Size: 1832 32 125
Fragments arranged by size:
1832 125 32
PpuMI rG'GwC_Cy
Cuts at: 0 194 885 1625 1865 1989
Size: 194 691 740 240 124
Fragments arranged by size:
740 691 240 194 124
PspOMI G'GGCC_C
Cuts at: 0 1531 1658 1989
Size: 1531 127 331
Fragments arranged by size:
1531 331 127
PstI C_TGCA'G
Cuts at: 0 1259 1989
Size: 1259 730
PvuII CAG'CTG
Cuts at: 0 75 100 382 1989
Size: 75 25 282 1607
Fragments arranged by size:
1607 282 75 25
SacI G_AGCT'C
Cuts at: 0 1706 1850 1989
Size: 1706 144 139
Fragments arranged by size:
1706 144 139
SacII CC_GC'GG
Cuts at: 0 284 1989
Size: 284 1705
SanDI GG'GwC_CC
Cuts at: 0 1865 1989
Size: 1865 124
SapI GCTCTTCn'nnn_
Cuts at: 0 656 1041 1989
Size: 656 385 948
Fragments arranged by size:
948 656 385
ScaI AGT'ACT
Cuts at: 0 1305 1989
Size: 1305 684
SexAI A'CCwGG_T
Cuts at: 0 887 1989
Size: 887 1102
SfcI C'TryA_G
Cuts at: 0 13 1255 1589 1989
Size: 13 1242 334 400
Fragments arranged by size:
1242 400 334 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 952 961 1989
Size: 952 9 1028
Fragments arranged by size:
1028 952 9
SfoI GGC'GCC
Cuts at: 0 1499 1989
Size: 1499 490
SmlI C'TyrA_G
Cuts at: 0 1843 1989
Size: 1843 146
SpeI A'CTAG_T
Cuts at: 0 448 1989
Size: 448 1541
StuI AGG'CCT
Cuts at: 0 484 946 1381 1989
Size: 484 462 435 608
Fragments arranged by size:
608 484 462 435
StyI C'CwwG_G
Cuts at: 0 542 881 1215 1600 1664 1989
Size: 542 339 334 385 64 325
Fragments arranged by size:
542 385 339 334 325 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1057 1518 1989
Size: 1057 461 471
Fragments arranged by size:
1057 471 461
TatI w'GTAC_w
Cuts at: 0 143 680 1303 1989
Size: 143 537 623 686
Fragments arranged by size:
686 623 537 143
Tth111I GACn'n_nGTC
Cuts at: 0 1794 1989
Size: 1794 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 888 957 1468 1989
Size: 318 570 69 511 521
Fragments arranged by size:
570 521 511 318 69
XmnI GAAnn'nnTTC
Cuts at: 0 356 1989
Size: 356 1633
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI ApoI AvaI AvrII
BaeI BanI BanII BbeI BbsI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrDI BstXI BstYI Bsu36I BtgI
CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NspI PasI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII
SanDI SapI ScaI SexAI SfcI SfiI SfoI SmlI
SpeI StuI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI BamHI
BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI
BsaBI BsiEI BsiWI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
DraI DraIII EagI EciI EcoRI FseI FspI FspAI
HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SbfI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI SwaI TstI
XbaI XhoI XmaI ZraI