Restriction map for pF1KB3124
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AccIII      AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BlnI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspEI       BspLU11I    BsrFI       BsrGI       BssHII      
BstBI       BstEII      BstPI       Bsu36I      Cfr10I      
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     FseI        FspI        HgaI        HindIII     
HpaI        KasI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AatII         101  GACGTC               GACGT^C
Acc65I       1292  GGTACC               G^GTACC
AccI          576  GTATAC               GT^[AC][GT]AC
AcyI          101  GACGTC               G[GA]^CG[CT]C
AflIII        110  ACGTGT               A^C[GA][CT]GT
AlwNI        1127  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I      1292  GGTACC               G^GTACC
AvaI         1108  CCCGAG               C^[CT]CG[GA]G
BamHI         936  GGATCC               G^GATCC
BanI         1292  GGTACC               G^G[CT][GA]CC
BclI          162  TGATCA               T^GATCA
BcnI          771  CCGGG                CC^[GC]GG
BsaBI         550  GATGAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         101  GACGTC               G[GA]^CG[CT]C
BsgI           30  CTGCAC               GTGCAG(16/14)
BsmI         1428  GAATGC               GAATGC(1/-1)
BsoBI        1108  CCCGAG               C^[CT]CG[GA]G
BspDI         943  ATCGAT               AT^CGAT
BspHI         818  TCATGA               T^CATGA
BsrBI         508  GAGCGG               CCGCTC(-3/-3)
BsrDI        1253  GCAATG               GCAATG(2/0)
Bst1107I      576  GTATAC               GTA^TAC
BstAPI       1214  GCAACGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1014  CCAGGGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       576  GTATAC               GTA^TAC
ClaI          943  ATCGAT               AT^CGAT
DraI          415  TTTAAA               TTT^AAA
DraII         909  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          707  CCGTGG               C^C[GA][CT]GG
EcoO109I      909  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         319  GAATTC               G^AATTC
EcoRV        1462  GATATC               GAT^ATC
EcoT14I       840  CCAAGG               C^C[AT][AT]GG
EcoT22I      1430  ATGCAT               ATGCA^T
FbaI          162  TGATCA               T^GATCA
HaeII          49  AGCGCC               [GA]GCGC^[CT]
HapII         771  CCGG                 C^CGG
Hin1I         101  GACGTC               G[GA]^CG[CT]C
HincII       1354  GTTGAC               GT[CT]^[GA]AC
HindII       1354  GTTGAC               GT[CT]^[GA]AC
HinfI         925  GAATC                G^A[ACGT]TC
HpaII         771  CCGG                 C^CGG
KpnI         1292  GGTACC               GGTAC^C
MamI          550  GATGAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          416  TTAA                 T^TAA
MslI          942  CATCGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          771  CCGG                 C^CGG
NciI          771  CCGGG                CC^[GC]GG
NdeI          380  CATATG               CA^TATG
NsiI         1430  ATGCAT               ATGCA^T
PpuMI         909  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1241  CTGCAG               CTGCA^G
RcaI          818  TCATGA               T^CATGA
SacI           70  GAGCTC               GAGCT^C
SapI         1399  GCTCTTC              GCTCTTC(1/4)
SfcI         1241  CTGCAG               C^T[GA][CT]AG
SpeI         1138  ACTAGT               A^CTAGT
StuI         1610  AGGCCT               AGG^CCT
StyI          840  CCAAGG               C^C[AT][AT]GG
TfiI          925  GAATC                G^A[AT]TC
Tru9I         416  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII          21  CGCG                 CG^CG
               54  CGCG                
AclI          440  AACGTT               AA^CGTT
             1216  AACGTT              
BalI          384  TGGCCA               TGG^CCA
              833  TGGCCA              
BanII          70  GAGCTC               G[GA]GC[CT]^C
              494  GGGCTC              
BbsI          375  GAAGAC               GAAGAC(2/6)
             1627  GTCTTC              
BfaI          904  CTAG                 C^TAG
             1139  CTAG                
BglII         173  AGATCT               A^GATCT
              420  AGATCT              
BlpI           45  GCTCAGC              GC^T[ACGT]AGC
              671  GCTTAGC             
BpmI          625  CTCCAG               CTGGAG(16/14)
              505  CTGGAG              
Bpu1102I       45  GCTCAGC              GC^T[ACGT]AGC
              671  GCTTAGC             
BpuAI         375  GAAGAC               GAAGAC(2/6)
             1627  GTCTTC              
BsaAI         109  TACGTG               [CT]AC^GT[GA]
              448  TACGTG              
BsaI          702  GGTCTC               GGTCTC(1/5)
              775  GGTCTC              
BseRI        1618  CTCCTC               GAGGAG(10/8)
              214  GAGGAG              
BsmFI         179  GTCCC                GGGAC(10/14)
              607  GTCCC               
BspMI         193  ACCTGC               ACCTGC(4/8)
             1289  GCAGGT              
BstUI          21  CGCG                 CG^CG
               54  CGCG                
CelII          45  GCTCAGC              GC^T[ACGT]AGC
              671  GCTTAGC             
EaeI          384  TGGCCA               [CT]^GGCC[GA]
              833  TGGCCA              
FauI           19  CCCGC                CCCGC(4/6)
              682  GCGGG               
MaeI          904  CTAG                 C^TAG
             1139  CTAG                
MluNI         384  TGGCCA               TGG^CCA
              833  TGGCCA              
MscI          384  TGGCCA               TGG^CCA
              833  TGGCCA              
MvnI           21  CGCG                 CG^CG
               54  CGCG                
NspI          395  ACATGC               [GA]CATG^[CT]
              974  ACATGC              
Psp1406I      440  AACGTT               AA^CGTT
             1216  AACGTT              
Tsp45I        781  GTGAC                ^GT[GC]AC
             1306  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BbvI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BssSI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TseI        Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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