Restriction map for pF1KB3124
 (Linear) (Six-base) MAPSORT of: KIBB3124.seq  Check: 7941  from: 1  to: 1668



KIBB3124.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AatII G_ACGT'C

Cuts at:      0     105    1668 
   Size:        105    1563
 
AccI GT'mk_AC

Cuts at:      0     577    1668 
   Size:        577    1091
 
Acc65I G'GTAC_C

Cuts at:      0    1292    1668 
   Size:       1292     376
 
AclI AA'CG_TT

Cuts at:      0     441    1217    1668 
   Size:        441     776     451

  Fragments arranged by size: 

                 776     451     441
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610    1065    1077    1508    1668 
   Size:        610     455      12     431     160

  Fragments arranged by size: 

                 610     455     431     160      12
 
AflIII A'CryG_T

Cuts at:      0     110    1668 
   Size:        110    1558
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     295    1668 
   Size:        295    1373
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     499     531    1668 
   Size:        499      32    1137

  Fragments arranged by size: 

                1137     499      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1132    1668 
   Size:       1132     536
 
ApoI r'AATT_y

Cuts at:      0     319     402    1052    1192    1668 
   Size:        319      83     650     140     476

  Fragments arranged by size: 

                 650     476     319     140      83
 
AvaI C'yCGr_G

Cuts at:      0    1108    1668 
   Size:       1108     560
 
BamHI G'GATC_C

Cuts at:      0     936    1668 
   Size:        936     732
 
BanI G'GyrC_C

Cuts at:      0    1292    1668 
   Size:       1292     376
 
BanII G_rGCy'C

Cuts at:      0      74     498    1668 
   Size:         74     424    1170

  Fragments arranged by size: 

                1170     424      74
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     382    1620    1668 
   Size:        382    1238      48

  Fragments arranged by size: 

                1238     382      48
 
BclI T'GATC_A

Cuts at:      0     162    1668 
   Size:        162    1506
 
BfrBI ATG'CAT

Cuts at:      0    1432    1668 
   Size:       1432     236
 
BglII A'GATC_T

Cuts at:      0     173     420    1668 
   Size:        173     247    1248

  Fragments arranged by size: 

                1248     247     173
 
BlpI GC'TnA_GC

Cuts at:      0      46     672    1668 
   Size:         46     626     996

  Fragments arranged by size: 

                 996     626      46
 
Bme1580I G_kGCm'C

Cuts at:      0    1522    1608    1668 
   Size:       1522      86      60

  Fragments arranged by size: 

                1522      86      60
 
BmgBI CAC'GTC

Cuts at:      0    1449    1668 
   Size:       1449     219
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     528     720    1668 
   Size:        528     192     948

  Fragments arranged by size: 

                 948     528     192
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526     610    1668 
   Size:        526      84    1058

  Fragments arranged by size: 

                1058     526      84
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     188     650    1668 
   Size:         58     130     462    1018

  Fragments arranged by size: 

                1018     462     130      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     708     781    1668 
   Size:        708      73     887

  Fragments arranged by size: 

                 887     708      73
 
BsaAI yAC'GTr

Cuts at:      0     111     450    1668 
   Size:        111     339    1218

  Fragments arranged by size: 

                1218     339     111
 
BsaBI GATnn'nnATC

Cuts at:      0     554    1668 
   Size:        554    1114
 
BsaHI Gr'CG_yC

Cuts at:      0     102    1668 
   Size:        102    1566
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     205     235     499     529     677     707    1668 
   Size:        205      30     264      30     148      30     961

  Fragments arranged by size: 

                 961     264     205     148      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     229    1609    1668 
   Size:        229    1380      59

  Fragments arranged by size: 

                1380     229      59
 
BseYI C'CCAG_C

Cuts at:      0    1041    1555    1668 
   Size:       1041     514     113

  Fragments arranged by size: 

                1041     514     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15    1668 
   Size:         15    1653
 
BsiHKAI G_wGCw'C

Cuts at:      0      47      74    1089    1668 
   Size:         47      27    1015     579

  Fragments arranged by size: 

                1015     579      47      27
 
BsmI GAATG_Cn'

Cuts at:      0    1434    1668 
   Size:       1434     234
 
Bsp1286I G_dGCh'C

Cuts at:      0      47      74     498    1089    1522    1608    1668 
   Size:         47      27     424     591     433      86      60

  Fragments arranged by size: 

                 591     433     424      86      60      47      27
 
BspHI T'CATG_A

Cuts at:      0     818    1668 
   Size:        818     850
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     202    1280    1668 
   Size:        202    1078     388

  Fragments arranged by size: 

                1078     388     202
 
BsrBI CCG'CTC

Cuts at:      0     510    1668 
   Size:        510    1158
 
BsrDI GCAATG_nn'

Cuts at:      0    1260    1668 
   Size:       1260     408
 
BssSI C'ACGA_G

Cuts at:      0     148     635     778    1668 
   Size:        148     487     143     890

  Fragments arranged by size: 

                 890     487     148     143
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1220    1668 
   Size:       1220     448
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1021    1668 
   Size:       1021     647
 
BstYI r'GATC_y

Cuts at:      0     173     420     846     936    1668 
   Size:        173     247     426      90     732

  Fragments arranged by size: 

                 732     426     247     173      90
 
BstZ17I GTA'TAC

Cuts at:      0     578    1668 
   Size:        578    1090
 
BtgI C'CryG_G

Cuts at:      0     707    1668 
   Size:        707     961
 
BtsI GCAGTG_nn'

Cuts at:      0      47     765    1310    1522    1668 
   Size:         47     718     545     212     146

  Fragments arranged by size: 

                 718     545     212     146      47
 
ClaI AT'CG_AT

Cuts at:      0     944    1668 
   Size:        944     724
 
DraI TTT'AAA

Cuts at:      0     417    1668 
   Size:        417    1251
 
EaeI y'GGCC_r

Cuts at:      0     384     833    1668 
   Size:        384     449     835

  Fragments arranged by size: 

                 835     449     384
 
EarI CTCTTCn'nnn_

Cuts at:      0     430    1406    1555    1668 
   Size:        430     976     149     113

  Fragments arranged by size: 

                 976     430     149     113
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     508    1668 
   Size:        508    1160
 
EcoICRI GAG'CTC

Cuts at:      0      72    1668 
   Size:         72    1596
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526     610    1065    1077    1508    1668 
   Size:        526      84     455      12     431     160

  Fragments arranged by size: 

                 526     455     431     160      84      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     910    1668 
   Size:        910     758
 
EcoRI G'AATT_C

Cuts at:      0     319    1668 
   Size:        319    1349
 
EcoRV GAT'ATC

Cuts at:      0    1464    1668 
   Size:       1464     204
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     352    1668 
   Size:        352    1316
 
HaeII r_GCGC'y

Cuts at:      0      53    1668 
   Size:         53    1615
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93    1371    1403    1668 
   Size:         61      32    1278      32     265

  Fragments arranged by size: 

                1278     265      61      32      32
 
HincII GTy'rAC

Cuts at:      0    1356    1668 
   Size:       1356     312
 
KpnI G_GTAC'C

Cuts at:      0    1296    1668 
   Size:       1296     372
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1249    1668 
   Size:       1249     419
 
MscI TGG'CCA

Cuts at:      0     386     835    1668 
   Size:        386     449     833

  Fragments arranged by size: 

                 833     449     386
 
MslI CAynn'nnrTG

Cuts at:      0     946    1668 
   Size:        946     722
 
MspA1I CmG'CkG

Cuts at:      0     588    1045    1132    1668 
   Size:        588     457      87     536

  Fragments arranged by size: 

                 588     536     457      87
 
NdeI CA'TA_TG

Cuts at:      0     381    1668 
   Size:        381    1287
 
NsiI A_TGCA'T

Cuts at:      0    1434    1668 
   Size:       1434     234
 
NspI r_CATG'y

Cuts at:      0     399     978    1668 
   Size:        399     579     690

  Fragments arranged by size: 

                 690     579     399
 
PasI CC'CwG_GG

Cuts at:      0     183    1668 
   Size:        183    1485
 
PfoI T'CCnGG_A

Cuts at:      0     503    1668 
   Size:        503    1165
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     499     531    1668 
   Size:        499      32    1137

  Fragments arranged by size: 

                1137     499      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     910    1668 
   Size:        910     758
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     520     552    1668 
   Size:        520      32    1116

  Fragments arranged by size: 

                1116     520      32
 
PstI C_TGCA'G

Cuts at:      0    1245    1668 
   Size:       1245     423
 
PvuII CAG'CTG

Cuts at:      0     588    1045    1132    1668 
   Size:        588     457      87     536

  Fragments arranged by size: 

                 588     536     457      87
 
SacI G_AGCT'C

Cuts at:      0      74    1668 
   Size:         74    1594
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1406    1668 
   Size:       1406     262
 
SfcI C'TryA_G

Cuts at:      0    1241    1668 
   Size:       1241     427
 
SmlI C'TyrA_G

Cuts at:      0      73     203     665    1668 
   Size:         73     130     462    1003

  Fragments arranged by size: 

                1003     462     130      73
 
SpeI A'CTAG_T

Cuts at:      0    1138    1668 
   Size:       1138     530
 
StuI AGG'CCT

Cuts at:      0    1612    1668 
   Size:       1612      56
 
StyI C'CwwG_G

Cuts at:      0     840    1668 
   Size:        840     828
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     918    1163    1668 
   Size:        918     245     505

  Fragments arranged by size: 

                 918     505     245
 
TatI w'GTAC_w

Cuts at:      0     221    1668 
   Size:        221    1447
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     677     709    1668 
   Size:        677      32     959

  Fragments arranged by size: 

                 959     677      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     595     868    1009    1668 
   Size:        595     273     141     659

  Fragments arranged by size: 

                 659     595     273     141
 
ZraI GAC'GTC

Cuts at:      0     103    1668 
   Size:        103    1565
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AlfI     AloI
    AlwNI     ApoI     AvaI    BamHI     BanI    BanII     BbsI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BssSI
   BstAPI    BstXI    BstYI  BstZ17I     BtgI     BtsI     ClaI     DraI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I   HincII     KpnI     MmeI     MscI     MslI
   MspA1I     NdeI     NsiI     NspI     PasI     PfoI     PpiI    PpuMI
     PsrI     PstI    PvuII     SacI     SapI     SfcI     SmlI     SpeI
     StuI     StyI    TaqII     TatI     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AgeI     AhdI     AleI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BciVI     BglI     BmtI     BplI   Bpu10I    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BsrFI    BsrGI   BssHII    BstBI   BstEII   Bsu36I
    BtgZI    CspCI   DraIII     DrdI     EagI    EcoNI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     MfeI     MluI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PciI    PflMI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI
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