(Circular) (Six-base) MAPSORT of: pF1KE0922.seq Check: 2165 from: 1 to: 5132 pF1KE0922 5132 bp With 182 enzymes: SgfI * April 24, 2009 13:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 393 1107 393 Size: 714 4418 AccI GT'mk_AC Cuts at: 2170 2170 Size: 5132 Acc65I G'GTAC_C Cuts at: 2148 2148 Size: 5132 AclI AA'CG_TT Cuts at: 4972 4972 Size: 5132 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 784 1081 1186 1378 1581 2113 2957 3389 4136 Size: 297 105 192 203 532 844 432 747 Cuts at: 4136 4404 784 Size: 268 1512 Fragments arranged by size: 1512 844 747 532 432 297 268 203 192 105 AfeI AGC'GCT Cuts at: 1220 1220 Size: 5132 AflIII A'CryG_T Cuts at: 1741 2484 3588 4565 1741 Size: 743 1104 977 2308 Fragments arranged by size: 2308 1104 977 743 AgeI A'CCGG_T Cuts at: 3520 3520 Size: 5132 AhdI GACnn_n'nnGTC Cuts at: 1359 1359 Size: 5132 AlwNI CAG_nnn'CTG Cuts at: 2437 4004 2437 Size: 1567 3565 ApaLI G'TGCA_C Cuts at: 3902 3902 Size: 5132 ApoI r'AATT_y Cuts at: 2026 2136 4485 4741 2026 Size: 110 2349 256 2417 Fragments arranged by size: 2417 2349 256 110 AseI AT'TA_AT Cuts at: 20 20 Size: 5132 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5132 AvaI C'yCGr_G Cuts at: 423 914 1154 1243 2152 423 Size: 491 240 89 909 3403 Fragments arranged by size: 3403 909 491 240 89 BamHI G'GATC_C Cuts at: 1604 2157 1604 Size: 553 4579 BanI G'GyrC_C Cuts at: 127 2148 2562 2810 2845 127 Size: 2021 414 248 35 2414 Fragments arranged by size: 2414 2021 414 248 35 BanII G_rGCy'C Cuts at: 145 1403 1579 2146 3176 145 Size: 1258 176 567 1030 2101 Fragments arranged by size: 2101 1258 1030 567 176 BbeI G_GCGC'C Cuts at: 2566 2814 2566 Size: 248 4884 BbsI GAAGACnn'nnnn_ Cuts at: 772 1057 1262 1785 1972 772 Size: 285 205 523 187 3932 Fragments arranged by size: 3932 523 285 205 187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 721 755 721 Size: 34 5098 BciVI GTATCCnnnnn_n' Cuts at: 862 1998 3030 3791 5132 862 Size: 1136 1032 761 1341 862 Fragments arranged by size: 1341 1136 1032 862 761 BclI T'GATC_A Cuts at: 1019 2652 1019 Size: 1633 3499 BfrBI ATG'CAT Cuts at: 1873 1873 Size: 5132 BglI GCCn_nnn'nGGC Cuts at: 1681 2104 1681 Size: 423 4709 BglII A'GATC_T Cuts at: 458 980 1367 1691 2647 4684 458 Size: 522 387 324 956 2037 906 Fragments arranged by size: 2037 956 906 522 387 324 BlpI GC'TnA_GC Cuts at: 2244 2244 Size: 5132 Bme1580I G_kGCm'C Cuts at: 2757 2850 3906 2757 Size: 93 1056 3983 Fragments arranged by size: 3983 1056 93 BmgBI CAC'GTC Cuts at: 1827 1827 Size: 5132 BmrI ACTGGGnnnn_n' Cuts at: 693 2344 2425 2760 4904 693 Size: 1651 81 335 2144 921 Fragments arranged by size: 2144 1651 921 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3499 3499 Size: 5132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 553 577 1394 1582 553 Size: 24 817 188 4103 Fragments arranged by size: 4103 817 188 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2308 3187 3679 3977 2308 Size: 879 492 298 3463 Fragments arranged by size: 3463 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 948 1370 4836 948 Size: 422 3466 1244 Fragments arranged by size: 3466 1244 422 BsaAI yAC'GTr Cuts at: 2485 2485 Size: 5132 BsaBI GATnn'nnATC Cuts at: 6 758 934 2646 4683 6 Size: 752 176 1712 2037 455 Fragments arranged by size: 2037 1712 752 455 176 BsaHI Gr'CG_yC Cuts at: 2563 2811 3513 5009 2563 Size: 248 702 1496 2686 Fragments arranged by size: 2686 1496 702 248 BsaWI w'CCGG_w Cuts at: 854 986 1784 2018 2080 2321 2842 3520 3794 Size: 132 798 234 62 241 521 678 274 Cuts at: 3794 3941 4352 854 Size: 147 411 1634 Fragments arranged by size: 1634 798 678 521 411 274 241 234 147 132 62 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 127 157 127 Size: 30 5102 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 622 1651 1658 622 Size: 1029 7 4096 Fragments arranged by size: 4096 1029 7 BseYI C'CCAG_C Cuts at: 1182 2557 3315 3892 4388 1182 Size: 1375 758 577 496 1926 Fragments arranged by size: 1926 1375 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1166 1166 Size: 5132 BsiEI CG_ry'CG Cuts at: 104 2674 2720 3928 104 Size: 2570 46 1208 1308 Fragments arranged by size: 2570 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 862 1579 1835 2146 2924 3114 3906 862 Size: 717 256 311 778 190 792 2088 Fragments arranged by size: 2088 792 778 717 311 256 190 Bsp1286I G_dGCh'C Cuts at: 145 862 1403 1579 1835 2146 2757 2850 2924 Size: 717 541 176 256 311 611 93 74 Cuts at: 2924 3114 3176 3906 145 Size: 190 62 730 1371 Fragments arranged by size: 1371 730 717 611 541 311 256 190 176 93 74 62 BspEI T'CCGG_A Cuts at: 854 986 1784 2018 2080 4352 854 Size: 132 798 234 62 2272 1634 Fragments arranged by size: 2272 1634 798 234 132 62 BspHI T'CATG_A Cuts at: 1403 1748 4308 1403 Size: 345 2560 2227 Fragments arranged by size: 2560 2227 345 BspMI ACCTGCnnnn'nnnn_ Cuts at: 393 1107 2015 2182 2396 2698 3079 393 Size: 714 908 167 214 302 381 2446 Fragments arranged by size: 2446 908 714 381 302 214 167 BsrBI CCG'CTC Cuts at: 50 525 3424 3478 4962 5127 50 Size: 475 2899 54 1484 165 55 Fragments arranged by size: 2899 1484 475 165 55 54 BsrDI GCAATG_nn' Cuts at: 317 1296 3044 317 Size: 979 1748 2405 Fragments arranged by size: 2405 1748 979 BsrFI r'CCGG_y Cuts at: 1413 1459 3130 3311 3520 1413 Size: 46 1671 181 209 3025 Fragments arranged by size: 3025 1671 209 181 46 BsrGI T'GTAC_A Cuts at: 194 194 Size: 5132 BssSI C'ACGA_G Cuts at: 3403 3761 3403 Size: 358 4774 BstAPI GCAn_nnn'nTGC Cuts at: 127 2509 127 Size: 2382 2750 BstBI TT'CG_AA Cuts at: 3493 3493 Size: 5132 BstXI CCAn_nnnn'nTGG Cuts at: 1983 1983 Size: 5132 BstYI r'GATC_y Cuts at: 1 458 980 1307 1367 1604 1691 2157 2624 Size: 457 522 327 60 237 87 466 467 Cuts at: 2624 2647 2982 3228 4240 4326 4338 4684 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 522 467 466 457 449 346 335 327 246 237 87 86 60 23 12 BtgI C'CryG_G Cuts at: 107 1448 1622 107 Size: 1341 174 3617 Fragments arranged by size: 3617 1341 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3082 3263 3426 3082 Size: 181 163 4788 Fragments arranged by size: 4788 181 163 BtsI GCAGTG_nn' Cuts at: 197 225 1154 2001 2375 197 Size: 28 929 847 374 2954 Fragments arranged by size: 2954 929 847 374 28 ClaI AT'CG_AT Cuts at: 1268 1268 Size: 5132 DraI TTT'AAA Cuts at: 329 2131 329 Size: 1802 3330 DraIII CAC_nnn'GTG Cuts at: 127 1717 127 Size: 1590 3542 DrdI GACnn_nn'nnGTC Cuts at: 1323 2838 3696 1323 Size: 1515 858 2759 Fragments arranged by size: 2759 1515 858 EaeI y'GGCC_r Cuts at: 110 561 836 1092 1335 1493 1590 1619 1880 Size: 451 275 256 243 158 97 29 261 Cuts at: 1880 2717 2891 3282 3309 4654 110 Size: 837 174 391 27 1345 588 Fragments arranged by size: 1345 837 588 451 391 275 261 256 243 174 158 97 29 27 EagI C'GGCC_G Cuts at: 2717 2717 Size: 5132 EarI CTCTTCn'nnn_ Cuts at: 892 1918 3155 3365 892 Size: 1026 1237 210 2659 Fragments arranged by size: 2659 1237 1026 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3340 3650 3796 4942 3340 Size: 310 146 1146 3530 Fragments arranged by size: 3530 1146 310 146 EcoICRI GAG'CTC Cuts at: 1577 2144 1577 Size: 567 4565 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 553 577 784 1081 1186 1378 1394 1581 1582 Size: 24 207 297 105 192 16 187 1 Cuts at: 1582 2113 2957 3389 4136 4404 553 Size: 531 844 432 747 268 1281 Fragments arranged by size: 1281 844 747 531 432 297 268 207 192 187 105 24 16 1 EcoNI CCTnn'n_nnAGG Cuts at: 592 661 1657 592 Size: 69 996 4067 Fragments arranged by size: 4067 996 69 EcoO109I rG'GnC_Cy Cuts at: 312 528 630 654 903 2271 312 Size: 216 102 24 249 1368 3173 Fragments arranged by size: 3173 1368 249 216 102 24 EcoRI G'AATT_C Cuts at: 2026 2136 2026 Size: 110 5022 EcoRV GAT'ATC Cuts at: 1272 1272 Size: 5132 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1347 1929 3151 1347 Size: 582 1222 3328 Fragments arranged by size: 3328 1222 582 FspI TGC'GCA Cuts at: 2913 2913 Size: 5132 HaeII r_GCGC'y Cuts at: 1222 2566 2814 3836 4815 1222 Size: 1344 248 1022 979 1539 Fragments arranged by size: 1539 1344 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 406 438 1345 1377 2974 3004 3006 3036 406 Size: 32 907 32 1597 30 2 30 2502 Fragments arranged by size: 2502 1597 907 32 32 30 30 2 HincII GTy'rAC Cuts at: 2171 2171 Size: 5132 KasI G'GCGC_C Cuts at: 2562 2810 2562 Size: 248 4884 KpnI G_GTAC'C Cuts at: 2152 2152 Size: 5132 MluI A'CGCG_T Cuts at: 4565 4565 Size: 5132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 938 3803 3987 938 Size: 2865 184 2083 Fragments arranged by size: 2865 2083 184 MscI TGG'CCA Cuts at: 112 563 1094 1495 1592 1621 1882 2893 112 Size: 451 531 401 97 29 261 1011 2351 Fragments arranged by size: 2351 1011 531 451 401 261 97 29 MslI CAynn'nnrTG Cuts at: 202 1424 1810 3121 3248 202 Size: 1222 386 1311 127 2086 Fragments arranged by size: 2086 1311 1222 386 127 MspA1I CmG'CkG Cuts at: 495 1299 2243 2557 2917 3930 4175 495 Size: 804 944 314 360 1013 245 1452 Fragments arranged by size: 1452 1013 944 804 360 314 245 NaeI GCC'GGC Cuts at: 1461 3313 1461 Size: 1852 3280 NarI GG'CG_CC Cuts at: 2563 2811 2563 Size: 248 4884 NcoI C'CATG_G Cuts at: 107 1448 107 Size: 1341 3791 NgoMIV G'CCGG_C Cuts at: 1459 3311 1459 Size: 1852 3280 NsiI A_TGCA'T Cuts at: 1875 1875 Size: 5132 NspI r_CATG'y Cuts at: 966 2185 2685 3592 966 Size: 1219 500 907 2506 Fragments arranged by size: 2506 1219 907 500 PciI A'CATG_T Cuts at: 3588 3588 Size: 5132 PflMI CCAn_nnn'nTGG Cuts at: 498 498 Size: 5132 PfoI T'CCnGG_A Cuts at: 469 650 1103 1796 469 Size: 181 453 693 3805 Fragments arranged by size: 3805 693 453 181 PmeI GTTT'AAAC Cuts at: 2131 2131 Size: 5132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 4271 Size: 32 5100 PpuMI rG'GwC_Cy Cuts at: 312 528 630 654 312 Size: 216 102 24 4790 Fragments arranged by size: 4790 216 102 24 PstI C_TGCA'G Cuts at: 2179 2864 2179 Size: 685 4447 PvuI CG_AT'CG Cuts at: 104 104 Size: 5132 PvuII CAG'CTG Cuts at: 495 2557 2917 495 Size: 2062 360 2710 Fragments arranged by size: 2710 2062 360 RsrII CG'GwC_CG Cuts at: 3327 3327 Size: 5132 SacI G_AGCT'C Cuts at: 1579 2146 1579 Size: 567 4565 SalI G'TCGA_C Cuts at: 2169 2169 Size: 5132 SapI GCTCTTCn'nnn_ Cuts at: 892 3155 3365 892 Size: 2263 210 2659 Fragments arranged by size: 2659 2263 210 SbfI CC_TGCA'GG Cuts at: 2179 2179 Size: 5132 ScaI AGT'ACT Cuts at: 4649 4649 Size: 5132 SexAI A'CCwGG_T Cuts at: 632 632 Size: 5132 SfcI C'TryA_G Cuts at: 33 1011 2175 2860 3853 4044 33 Size: 978 1164 685 993 191 1121 Fragments arranged by size: 1164 1121 993 978 685 191 SfoI GGC'GCC Cuts at: 2564 2812 2564 Size: 248 4884 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5132 SmaI CCC'GGG Cuts at: 2154 2154 Size: 5132 SmlI C'TyrA_G Cuts at: 2287 3202 3694 3956 2287 Size: 915 492 262 3463 Fragments arranged by size: 3463 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5132 SphI G_CATG'C Cuts at: 2185 2685 2185 Size: 500 4632 SspI AAT'ATT Cuts at: 871 871 Size: 5132 StuI AGG'CCT Cuts at: 1534 2098 1534 Size: 564 4568 StyI C'CwwG_G Cuts at: 107 1448 1733 2266 2620 107 Size: 1341 285 533 354 2619 Fragments arranged by size: 2619 1341 533 354 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2715 2848 3307 3516 2715 Size: 133 459 209 4331 Fragments arranged by size: 4331 459 209 133 TatI w'GTAC_w Cuts at: 194 1043 4647 194 Size: 849 3604 679 Fragments arranged by size: 3604 849 679 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4649 4681 4649 Size: 32 5100 Tth111I GACn'n_nGTC Cuts at: 2929 2929 Size: 5132 XbaI T'CTAG_A Cuts at: 2163 2163 Size: 5132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1824 2627 1824 Size: 803 4329 XmaI C'CCGG_G Cuts at: 2152 2152 Size: 5132 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BmtI Bpu10I BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI