Restriction map for
pF1KB3940
(Circular) (Six-base) MAPSORT of: pF1KB3940.seq Check: 1660 from: 1 to: 3626
pF1KB3940 3626 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 536 664 536
Size: 128 3498
Acc65I G'GTAC_C
Cuts at: 642 642
Size: 3626
AclI AA'CG_TT
Cuts at: 3466 3466
Size: 3626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1451 1883 2630 2898 210
Size: 1241 432 747 268 938
Fragments arranged by size:
1241 938 747 432 268
AflIII A'CryG_T
Cuts at: 978 2082 3059 978
Size: 1104 977 1545
Fragments arranged by size:
1545 1104 977
AgeI A'CCGG_T
Cuts at: 2014 2014
Size: 3626
AleI CACnn'nnGTG
Cuts at: 523 523
Size: 3626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 541 541
Size: 3626
AlwNI CAG_nnn'CTG
Cuts at: 237 246 387 931 2498 237
Size: 9 141 544 1567 1365
Fragments arranged by size:
1567 1365 544 141 9
ApaLI G'TGCA_C
Cuts at: 2396 2396
Size: 3626
ApoI r'AATT_y
Cuts at: 630 2979 3235 630
Size: 2349 256 1021
Fragments arranged by size:
2349 1021 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3626
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
AvaI C'yCGr_G
Cuts at: 125 646 125
Size: 521 3105
BamHI G'GATC_C
Cuts at: 336 651 336
Size: 315 3311
BanI G'GyrC_C
Cuts at: 642 1056 1304 1339 642
Size: 414 248 35 2929
Fragments arranged by size:
2929 414 248 35
BanII G_rGCy'C
Cuts at: 200 586 640 1670 200
Size: 386 54 1030 2156
Fragments arranged by size:
2156 1030 386 54
BbeI G_GCGC'C
Cuts at: 1060 1308 1060
Size: 248 3378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3592
BciVI GTATCCnnnnn_n'
Cuts at: 1524 2285 3626 1524
Size: 761 1341 1524
Fragments arranged by size:
1524 1341 761
BclI T'GATC_A
Cuts at: 1146 1146
Size: 3626
BfrBI ATG'CAT
Cuts at: 111 111
Size: 3626
BglII A'GATC_T
Cuts at: 1141 3178 1141
Size: 2037 1589
BlpI GC'TnA_GC
Cuts at: 738 738
Size: 3626
Bme1580I G_kGCm'C
Cuts at: 1251 1344 2400 1251
Size: 93 1056 2477
Fragments arranged by size:
2477 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 838 919 1254 3398 838
Size: 81 335 2144 1066
Fragments arranged by size:
2144 1066 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 480 1993 480
Size: 1513 2113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1681 2173 2471 802
Size: 879 492 298 1957
Fragments arranged by size:
1957 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3330 3330
Size: 3626
BsaAI yAC'GTr
Cuts at: 979 979
Size: 3626
BsaBI GATnn'nnATC
Cuts at: 6 1140 3177 6
Size: 1134 2037 455
Fragments arranged by size:
2037 1134 455
BsaHI Gr'CG_yC
Cuts at: 1057 1305 2007 3503 1057
Size: 248 702 1496 1180
Fragments arranged by size:
1496 1180 702 248
BsaWI w'CCGG_w
Cuts at: 815 1336 2014 2288 2435 2846 815
Size: 521 678 274 147 411 1595
Fragments arranged by size:
1595 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1051 1809 2386 2882 1051
Size: 758 577 496 1795
Fragments arranged by size:
1795 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1168 1214 2422 104
Size: 1064 46 1208 1308
Fragments arranged by size:
1308 1208 1064 46
BsiHKAI G_wGCw'C
Cuts at: 640 1418 1608 2400 640
Size: 778 190 792 1866
Fragments arranged by size:
1866 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 200 586 640 1251 1344 1418 1608 1670 2400
Size: 386 54 611 93 74 190 62 730
Cuts at: 2400 200
Size: 1426
Fragments arranged by size:
1426 730 611 386 190 93 74 62
54
BspEI T'CCGG_A
Cuts at: 2846 2846
Size: 3626
BspHI T'CATG_A
Cuts at: 2802 2802
Size: 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 676 890 1192 1573 676
Size: 214 302 381 2729
Fragments arranged by size:
2729 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1918 1972 3456 3621 50
Size: 1868 54 1484 165 55
Fragments arranged by size:
1868 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1538 1538
Size: 3626
BsrFI r'CCGG_y
Cuts at: 1624 1805 2014 1624
Size: 181 209 3236
Fragments arranged by size:
3236 209 181
BssSI C'ACGA_G
Cuts at: 201 1897 2255 201
Size: 1696 358 1572
Fragments arranged by size:
1696 1572 358
BstAPI GCAn_nnn'nTGC
Cuts at: 246 1003 246
Size: 757 2869
BstBI TT'CG_AA
Cuts at: 1987 1987
Size: 3626
BstEII G'GTnAC_C
Cuts at: 304 304
Size: 3626
BstYI r'GATC_y
Cuts at: 1 336 651 1118 1141 1476 1722 2734 2820
Size: 335 315 467 23 335 246 1012 86
Cuts at: 2820 2832 3178 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 335 315 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1576 1757 1920 1576
Size: 181 163 3282
Fragments arranged by size:
3282 181 163
BtsI GCAGTG_nn'
Cuts at: 869 869
Size: 3626
DraI TTT'AAA
Cuts at: 625 625
Size: 3626
DrdI GACnn_nn'nnGTC
Cuts at: 377 1332 2190 377
Size: 955 858 1813
Fragments arranged by size:
1813 955 858
EaeI y'GGCC_r
Cuts at: 1211 1385 1776 1803 3148 1211
Size: 174 391 27 1345 1689
Fragments arranged by size:
1689 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1211 1211
Size: 3626
EarI CTCTTCn'nnn_
Cuts at: 1649 1859 1649
Size: 210 3416
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1834 2144 2290 3436 1834
Size: 310 146 1146 2024
Fragments arranged by size:
2024 1146 310 146
EcoICRI GAG'CTC
Cuts at: 638 638
Size: 3626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1451 1883 2630 2898 210
Size: 1241 432 747 268 938
Fragments arranged by size:
1241 938 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 765 765
Size: 3626
EcoRI G'AATT_C
Cuts at: 630 630
Size: 3626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1645 1645
Size: 3626
FspI TGC'GCA
Cuts at: 1407 1407
Size: 3626
HaeII r_GCGC'y
Cuts at: 1060 1308 2330 3309 1060
Size: 248 1022 979 1377
Fragments arranged by size:
1377 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1468 1498 1500 1530 1468
Size: 30 2 30 3564
Fragments arranged by size:
3564 30 30 2
HincII GTy'rAC
Cuts at: 426 665 426
Size: 239 3387
KasI G'GCGC_C
Cuts at: 1056 1304 1056
Size: 248 3378
KpnI G_GTAC'C
Cuts at: 646 646
Size: 3626
MluI A'CGCG_T
Cuts at: 3059 3059
Size: 3626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2297 2481 2297
Size: 184 3442
MscI TGG'CCA
Cuts at: 1387 1387
Size: 3626
MslI CAynn'nnrTG
Cuts at: 472 523 1615 1742 472
Size: 51 1092 127 2356
Fragments arranged by size:
2356 1092 127 51
MspA1I CmG'CkG
Cuts at: 216 363 551 737 1051 1411 2424 2669 216
Size: 147 188 186 314 360 1013 245 1173
Fragments arranged by size:
1173 1013 360 314 245 188 186 147
NaeI GCC'GGC
Cuts at: 1807 1807
Size: 3626
NarI GG'CG_CC
Cuts at: 1057 1305 1057
Size: 248 3378
NgoMIV G'CCGG_C
Cuts at: 1805 1805
Size: 3626
NsiI A_TGCA'T
Cuts at: 113 113
Size: 3626
NspI r_CATG'y
Cuts at: 679 1179 2086 679
Size: 500 907 2219
Fragments arranged by size:
2219 907 500
PciI A'CATG_T
Cuts at: 2082 2082
Size: 3626
PflMI CCAn_nnn'nTGG
Cuts at: 333 333
Size: 3626
PmeI GTTT'AAAC
Cuts at: 625 625
Size: 3626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 401 433 2765 2797 401
Size: 32 2332 32 1230
Fragments arranged by size:
2332 1230 32 32
PsiI TTA'TAA
Cuts at: 263 263
Size: 3626
PstI C_TGCA'G
Cuts at: 221 242 673 1358 221
Size: 21 431 685 2489
Fragments arranged by size:
2489 685 431 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3626
PvuII CAG'CTG
Cuts at: 216 551 1051 1411 216
Size: 335 500 360 2431
Fragments arranged by size:
2431 500 360 335
RsrII CG'GwC_CG
Cuts at: 1821 1821
Size: 3626
SacI G_AGCT'C
Cuts at: 640 640
Size: 3626
SalI G'TCGA_C
Cuts at: 663 663
Size: 3626
SapI GCTCTTCn'nnn_
Cuts at: 1649 1859 1649
Size: 210 3416
SbfI CC_TGCA'GG
Cuts at: 242 673 242
Size: 431 3195
ScaI AGT'ACT
Cuts at: 3143 3143
Size: 3626
SfcI C'TryA_G
Cuts at: 33 217 238 444 669 1354 2347 2538 33
Size: 184 21 206 225 685 993 191 1121
Fragments arranged by size:
1121 993 685 225 206 191 184 21
SfoI GGC'GCC
Cuts at: 1058 1306 1058
Size: 248 3378
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
SmaI CCC'GGG
Cuts at: 127 648 127
Size: 521 3105
SmlI C'TyrA_G
Cuts at: 781 1696 2188 2450 781
Size: 915 492 262 1957
Fragments arranged by size:
1957 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3626
SphI G_CATG'C
Cuts at: 679 1179 679
Size: 500 3126
StyI C'CwwG_G
Cuts at: 191 760 1114 191
Size: 569 354 2703
Fragments arranged by size:
2703 569 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1209 1342 1801 2010 1209
Size: 133 459 209 2825
Fragments arranged by size:
2825 459 209 133
TatI w'GTAC_w
Cuts at: 3141 3141
Size: 3626
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3594
Tth111I GACn'n_nGTC
Cuts at: 1423 1423
Size: 3626
XbaI T'CTAG_A
Cuts at: 657 657
Size: 3626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 528 1121 528
Size: 593 3033
XmaI C'CCGG_G
Cuts at: 125 646 125
Size: 521 3105
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI