Restriction map for pF1KB6907
 (Linear) (Six-base) MAPSORT of: KIBB6907.seq  Check: 2550  from: 1  to: 612



KIBB6907.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     612 
   Size:        331     281
 
AhdI GACnn_n'nnGTC

Cuts at:      0     567     612 
   Size:        567      45
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     454     486     612 
   Size:        454      32     126

  Fragments arranged by size: 

                 454     126      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     612 
   Size:        120     492
 
ApaI G_GGCC'C

Cuts at:      0     253     452     612 
   Size:        253     199     160

  Fragments arranged by size: 

                 253     199     160
 
AvaI C'yCGr_G

Cuts at:      0     246     452     512     612 
   Size:        246     206      60     100

  Fragments arranged by size: 

                 246     206     100      60
 
BanI G'GyrC_C

Cuts at:      0     492     612 
   Size:        492     120
 
BanII G_rGCy'C

Cuts at:      0      17     253     452     612 
   Size:         17     236     199     160

  Fragments arranged by size: 

                 236     199     160      17
 
BlpI GC'TnA_GC

Cuts at:      0       5     612 
   Size:          5     607
 
Bme1580I G_kGCm'C

Cuts at:      0     253     452     612 
   Size:        253     199     160

  Fragments arranged by size: 

                 253     199     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     301     612 
   Size:        277      24     311

  Fragments arranged by size: 

                 311     277      24
 
BsaAI yAC'GTr

Cuts at:      0     395     612 
   Size:        395     217
 
BsaBI GATnn'nnATC

Cuts at:      0     337     612 
   Size:        337     275
 
BsaHI Gr'CG_yC

Cuts at:      0     407     612 
   Size:        407     205
 
BsaWI w'CCGG_w

Cuts at:      0     329     612 
   Size:        329     283
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     454     484     612 
   Size:        454      30     128

  Fragments arranged by size: 

                 454     128      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     475     502     612 
   Size:         70     405      27     110

  Fragments arranged by size: 

                 405     110      70      27
 
BseYI C'CCAG_C

Cuts at:      0     165     612 
   Size:        165     447
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     612 
   Size:        255     357
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     612 
   Size:         68     544
 
Bsp1286I G_dGCh'C

Cuts at:      0      17      68     253     452     612 
   Size:         17      51     185     199     160

  Fragments arranged by size: 

                 199     185     160      51      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     612 
   Size:        210     402
 
BstYI r'GATC_y

Cuts at:      0     110     612 
   Size:        110     502
 
BtgI C'CryG_G

Cuts at:      0     482     612 
   Size:        482     130
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     196     612 
   Size:        196     416
 
EaeI y'GGCC_r

Cuts at:      0     176     612 
   Size:        176     436
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     612 
   Size:         91     521
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     301     331     612 
   Size:        277      24      30     281

  Fragments arranged by size: 

                 281     277      30      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159     250     449     612 
   Size:        159      91     199     163

  Fragments arranged by size: 

                 199     163     159      91
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     555     612 
   Size:        555      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84     352     384     553     585     612 
   Size:         52      32     268      32     169      32      27

  Fragments arranged by size: 

                 268     169      52      32      32      32      27
 
HincII GTy'rAC

Cuts at:      0      75     354     612 
   Size:         75     279     258

  Fragments arranged by size: 

                 279     258      75
 
HpaI GTT'AAC

Cuts at:      0     354     612 
   Size:        354     258
 
MspA1I CmG'CkG

Cuts at:      0      30      46     612 
   Size:         30      16     566

  Fragments arranged by size: 

                 566      30      16
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     120     612 
   Size:        120     492
 
PfoI T'CCnGG_A

Cuts at:      0     104     113     612 
   Size:        104       9     499

  Fragments arranged by size: 

                 499     104       9
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     612 
   Size:        454      32     126

  Fragments arranged by size: 

                 454     126      32
 
PspOMI G'GGCC_C

Cuts at:      0     249     448     612 
   Size:        249     199     164

  Fragments arranged by size: 

                 249     199     164
 
PvuII CAG'CTG

Cuts at:      0      30     612 
   Size:         30     582
 
SapI GCTCTTCn'nnn_

Cuts at:      0      91     612 
   Size:         91     521
 
SfcI C'TryA_G

Cuts at:      0     522     612 
   Size:        522      90
 
SmaI CCC'GGG

Cuts at:      0     248     454     514     612 
   Size:        248     206      60      98

  Fragments arranged by size: 

                 248     206      98      60
 
SnaBI TAC'GTA

Cuts at:      0     395     612 
   Size:        395     217
 
StyI C'CwwG_G

Cuts at:      0     497     612 
   Size:        497     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      60     510     574     612 
   Size:         60     450      64      38

  Fragments arranged by size: 

                 450      64      60      38
 
TatI w'GTAC_w

Cuts at:      0     149     380     612 
   Size:        149     231     232

  Fragments arranged by size: 

                 232     231     149
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     504     612 
   Size:        504     108
 
XmaI C'CCGG_G

Cuts at:      0     246     452     512     612 
   Size:        246     206      60     100

  Fragments arranged by size: 

                 246     206     100      60
 

 Enzymes that do cut:

     AcuI     AhdI     AloI    AlwNI     ApaI     AvaI     BanI    BanII
     BlpI Bme1580I     BpmI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BstXI    BstYI     BtgI
     DrdI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII
     HpaI   MspA1I    PflMI     PfoI     PpiI   PspOMI    PvuII     SapI
     SfcI     SmaI    SnaBI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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