Restriction map for pF1KE1596
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AflII       AhdI        Alw44I      AlwNI       Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbeI        BbsI        
BcgI        BciVI       BclI        BcnI        BfrI        
BlnI        BpuAI       BsaBI       BsgI        BsiEI       
BsiWI       BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     CfoI        ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       FauI        FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MboII       
MfeI        MluI        MluNI       MscI        MseI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PflFI       PflMI       
PmeI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspI        
SwaI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          706  GTCTAC               GT^[AC][GT]AC
AccIII       1031  TCCGGA               T^CCGGA
AcyI         1037  GACGCC               G[GA]^CG[CT]C
AflIII        479  ACACGT               A^C[GA][CT]GT
AgeI          950  ACCGGT               A^CCGGT
AlwI          984  GATCC                GGATC(4/5)
AocI          882  CCTGAGG              CC^T[ACGT]AGG
ApaI           26  GGGCCC               GGGCC^C
AspHI         132  GAGCAC               G[AT]GC[AT]^C
BanII          26  GGGCCC               G[GA]GC[CT]^C
BbrPI         480  CACGTG               CAC^GTG
BbvCI         666  CCTCAGC              CCTCAGC(-5/-2)
BglI         1048  GCCTACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         795  AGATCT               A^GATCT
BlpI          898  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      898  GCTCAGC              GC^T[ACGT]AGC
BsaAI         480  CACGTG               [CT]AC^GT[GA]
BsaHI        1037  GACGCC               G[GA]^CG[CT]C
BsaI          463  GAGACC               GGTCTC(1/5)
BseAI        1031  TCCGGA               T^CCGGA
BsiHKAI       132  GAGCAC               G[AT]GC[AT]^C
BsmBI        1035  GAGACG               CGTCTC(1/5)
BsmI          780  GAATGC               GAATGC(1/-1)
Bsp1407I      542  TGTACA               T^GTACA
BspEI        1031  TCCGGA               T^CCGGA
BsrFI         950  ACCGGT               [GA]^CCGG[CT]
BsrGI         542  TGTACA               T^GTACA
BstYI         795  AGATCT               [GA]^GATC[CT]
Bsu36I        882  CCTGAGG              CC^T[ACGT]AGG
Cac8I           6  GCTGGC               GC[ACGT]^[ACGT]GC
CelII         898  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        950  ACCGGT               [GA]^CCGG[CT]
Eco81I        882  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       782  ATGCAT               ATGCA^T
HgaI         1037  GACGC                GACGC(5/10)
Hin1I        1037  GACGCC               G[GA]^CG[CT]C
MflI          795  AGATCT               [GA]^GATC[CT]
MroI         1031  TCCGGA               T^CCGGA
NcoI          255  CCATGG               C^CATGG
NsiI          782  ATGCAT               ATGCA^T
PaeR7I        837  CTCGAG               C^TCGAG
PinAI         950  ACCGGT               A^CCGGT
PmaCI         480  CACGTG               CAC^GTG
PmlI          480  CACGTG               CAC^GTG
SmlI          837  CTCGAG               C^T[CT][GA]AG
SpeI          340  ACTAGT               A^CTAGT
SspBI         542  TGTACA               T^GTACA
StuI          566  AGGCCT               AGG^CCT
XhoI          837  CTCGAG               C^TCGAG
XhoII         795  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI         1046  CCGC                 CCGC(-3/-1)
              724  GCGG                
AvaI           21  CTCGGG               C^[CT]CG[GA]G
              837  CTCGAG              
BanI          202  GGCACC               G^G[CT][GA]CC
              625  GGTGCC              
BfaI          341  CTAG                 C^TAG
              446  CTAG                
BpmI          691  CTCCAG               CTGGAG(16/14)
              889  CTCCAG              
BsaWI         950  ACCGGT               [AT]^CCGG[AT]
             1031  TCCGGA              
BseRI         997  CTCCTC               GAGGAG(10/8)
              560  GAGGAG              
BsmFI          55  GGGAC                GGGAC(10/14)
              306  GGGAC               
BsoBI          21  CTCGGG               C^[CT]CG[GA]G
              837  CTCGAG              
BspMI         638  ACCTGC               ACCTGC(4/8)
              872  ACCTGC              
DpnII         796  GATC                 ^GATC
              984  GATC                
HapII         951  CCGG                 C^CGG
             1032  CCGG                
HpaII         951  CCGG                 C^CGG
             1032  CCGG                
MaeI          341  CTAG                 C^TAG
              446  CTAG                
MaeII         263  ACGT                 A^CGT
              481  ACGT                
MboI          796  GATC                 ^GATC
              984  GATC                
MslI          346  CATGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              720  CATGGCGGTG          
MspA1I        857  CAGCTG               C[AC]G^C[GT]G
              901  CAGCTG              
MspI          951  CCGG                 C^CGG
             1032  CCGG                
NdeII         796  GATC                 ^GATC
              984  GATC                
PleI          441  GAGTC                GAGTC(4/5)
              536  GAGTC               
PpuMI         306  GGGACCC              [GA]G^G[AT]CC[CT]
              686  AGGACCT             
PvuII         857  CAGCTG               CAG^CTG
              901  CAGCTG              
Sau3AI        796  GATC                 ^GATC
              984  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AfaI        AluI        AvaII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeIII      MnlI        MvaI        
MwoI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     
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