(Circular) (Six-base) MAPSORT of: pF1KE1084.seq Check: 2638 from: 1 to: 3749 pF1KE1084 3749 bp With 182 enzymes: SgfI * June 30, 2009 14:14 .. AccI GT'mk_AC Cuts at: 479 787 479 Size: 308 3441 Acc65I G'GTAC_C Cuts at: 765 765 Size: 3749 AclI AA'CG_TT Cuts at: 3589 3589 Size: 3749 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 239 1574 2006 2753 3021 239 Size: 1335 432 747 268 967 Fragments arranged by size: 1335 967 747 432 268 AflIII A'CryG_T Cuts at: 319 335 1101 2205 3182 319 Size: 16 766 1104 977 886 Fragments arranged by size: 1104 977 886 766 16 AgeI A'CCGG_T Cuts at: 2137 2137 Size: 3749 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 103 135 103 Size: 32 3717 AlwNI CAG_nnn'CTG Cuts at: 567 663 1054 2621 567 Size: 96 391 1567 1695 Fragments arranged by size: 1695 1567 391 96 ApaLI G'TGCA_C Cuts at: 2519 2519 Size: 3749 ApoI r'AATT_y Cuts at: 275 753 3102 3358 275 Size: 478 2349 256 666 Fragments arranged by size: 2349 666 478 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3749 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3749 AvaI C'yCGr_G Cuts at: 769 769 Size: 3749 BamHI G'GATC_C Cuts at: 774 774 Size: 3749 BanI G'GyrC_C Cuts at: 765 1179 1427 1462 765 Size: 414 248 35 3052 Fragments arranged by size: 3052 414 248 35 BanII G_rGCy'C Cuts at: 732 763 1793 732 Size: 31 1030 2688 Fragments arranged by size: 2688 1030 31 BbeI G_GCGC'C Cuts at: 1183 1431 1183 Size: 248 3501 BciVI GTATCCnnnnn_n' Cuts at: 1647 2408 3749 1647 Size: 761 1341 1647 Fragments arranged by size: 1647 1341 761 BclI T'GATC_A Cuts at: 1269 1269 Size: 3749 BglII A'GATC_T Cuts at: 1264 3301 1264 Size: 2037 1712 BlpI GC'TnA_GC Cuts at: 861 861 Size: 3749 Bme1580I G_kGCm'C Cuts at: 1374 1467 2523 1374 Size: 93 1056 2600 Fragments arranged by size: 2600 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 187 961 1042 1377 3521 187 Size: 774 81 335 2144 415 Fragments arranged by size: 2144 774 415 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2116 2116 Size: 3749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 478 478 Size: 3749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 925 1804 2296 2594 925 Size: 879 492 298 2080 Fragments arranged by size: 2080 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3453 3453 Size: 3749 BsaAI yAC'GTr Cuts at: 1102 1102 Size: 3749 BsaBI GATnn'nnATC Cuts at: 6 1263 3300 6 Size: 1257 2037 455 Fragments arranged by size: 2037 1257 455 BsaHI Gr'CG_yC Cuts at: 1180 1428 2130 3626 1180 Size: 248 702 1496 1303 Fragments arranged by size: 1496 1303 702 248 BsaWI w'CCGG_w Cuts at: 938 1459 2137 2411 2558 2969 938 Size: 521 678 274 147 411 1718 Fragments arranged by size: 1718 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 103 133 103 Size: 30 3719 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 445 445 Size: 3749 BseYI C'CCAG_C Cuts at: 1174 1932 2509 3005 1174 Size: 758 577 496 1918 Fragments arranged by size: 1918 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 556 556 Size: 3749 BsiEI CG_ry'CG Cuts at: 104 1291 1337 2545 104 Size: 1187 46 1208 1308 Fragments arranged by size: 1308 1208 1187 46 BsiHKAI G_wGCw'C Cuts at: 763 1541 1731 2523 763 Size: 778 190 792 1989 Fragments arranged by size: 1989 792 778 190 Bsp1286I G_dGCh'C Cuts at: 732 763 1374 1467 1541 1731 1793 2523 732 Size: 31 611 93 74 190 62 730 1958 Fragments arranged by size: 1958 730 611 190 93 74 62 31 BspEI T'CCGG_A Cuts at: 2969 2969 Size: 3749 BspHI T'CATG_A Cuts at: 2925 2925 Size: 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 799 1013 1315 1696 799 Size: 214 302 381 2852 Fragments arranged by size: 2852 381 302 214 BsrBI CCG'CTC Cuts at: 50 2041 2095 3579 3744 50 Size: 1991 54 1484 165 55 Fragments arranged by size: 1991 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1661 1661 Size: 3749 BsrFI r'CCGG_y Cuts at: 1747 1928 2137 1747 Size: 181 209 3359 Fragments arranged by size: 3359 209 181 BssSI C'ACGA_G Cuts at: 2020 2378 2020 Size: 358 3391 BstAPI GCAn_nnn'nTGC Cuts at: 1126 1126 Size: 3749 BstBI TT'CG_AA Cuts at: 2110 2110 Size: 3749 BstYI r'GATC_y Cuts at: 1 774 1241 1264 1599 1845 2857 2943 2955 Size: 773 467 23 335 246 1012 86 12 Cuts at: 2955 3301 1 Size: 346 449 Fragments arranged by size: 1012 773 467 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 480 480 Size: 3749 Bsu36I CC'TnA_GG Cuts at: 724 724 Size: 3749 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1699 1880 2043 1699 Size: 181 163 3405 Fragments arranged by size: 3405 181 163 BtsI GCAGTG_nn' Cuts at: 992 992 Size: 3749 DraI TTT'AAA Cuts at: 748 748 Size: 3749 DrdI GACnn_nn'nnGTC Cuts at: 1455 2313 1455 Size: 858 2891 EaeI y'GGCC_r Cuts at: 1334 1508 1899 1926 3271 1334 Size: 174 391 27 1345 1812 Fragments arranged by size: 1812 1345 391 174 27 EagI C'GGCC_G Cuts at: 1334 1334 Size: 3749 EarI CTCTTCn'nnn_ Cuts at: 258 737 1772 1982 258 Size: 479 1035 210 2025 Fragments arranged by size: 2025 1035 479 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1957 2267 2413 3559 1957 Size: 310 146 1146 2147 Fragments arranged by size: 2147 1146 310 146 EcoICRI GAG'CTC Cuts at: 761 761 Size: 3749 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 239 478 1574 2006 2753 3021 239 Size: 239 1096 432 747 268 967 Fragments arranged by size: 1096 967 747 432 268 239 EcoNI CCTnn'n_nnAGG Cuts at: 310 310 Size: 3749 EcoO109I rG'GnC_Cy Cuts at: 888 888 Size: 3749 EcoRI G'AATT_C Cuts at: 753 753 Size: 3749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1768 1768 Size: 3749 FspI TGC'GCA Cuts at: 694 1530 694 Size: 836 2913 HaeII r_GCGC'y Cuts at: 131 1183 1431 2453 3432 131 Size: 1052 248 1022 979 448 Fragments arranged by size: 1052 1022 979 448 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 230 262 283 315 666 698 1591 1621 1623 Size: 32 21 32 351 32 893 30 2 Cuts at: 1623 1653 230 Size: 30 2326 Fragments arranged by size: 2326 893 351 32 32 32 30 30 21 2 HincII GTy'rAC Cuts at: 788 788 Size: 3749 KasI G'GCGC_C Cuts at: 1179 1427 1179 Size: 248 3501 KpnI G_GTAC'C Cuts at: 769 769 Size: 3749 MluI A'CGCG_T Cuts at: 3182 3182 Size: 3749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2420 2604 2420 Size: 184 3565 MscI TGG'CCA Cuts at: 1510 1510 Size: 3749 MslI CAynn'nnrTG Cuts at: 1738 1865 1738 Size: 127 3622 MspA1I CmG'CkG Cuts at: 860 1174 1534 2547 2792 860 Size: 314 360 1013 245 1817 Fragments arranged by size: 1817 1013 360 314 245 NaeI GCC'GGC Cuts at: 1930 1930 Size: 3749 NarI GG'CG_CC Cuts at: 1180 1428 1180 Size: 248 3501 NgoMIV G'CCGG_C Cuts at: 1928 1928 Size: 3749 NspI r_CATG'y Cuts at: 323 339 802 1302 2209 323 Size: 16 463 500 907 1863 Fragments arranged by size: 1863 907 500 463 16 PasI CC'CwG_GG Cuts at: 153 153 Size: 3749 PciI A'CATG_T Cuts at: 319 335 2205 319 Size: 16 1870 1863 Fragments arranged by size: 1870 1863 16 PmeI GTTT'AAAC Cuts at: 748 748 Size: 3749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 103 135 2888 2920 103 Size: 32 2753 32 932 Fragments arranged by size: 2753 932 32 32 PstI C_TGCA'G Cuts at: 572 647 668 716 796 1481 572 Size: 75 21 48 80 685 2840 Fragments arranged by size: 2840 685 80 75 48 21 PvuI CG_AT'CG Cuts at: 104 104 Size: 3749 PvuII CAG'CTG Cuts at: 1174 1534 1174 Size: 360 3389 RsrII CG'GwC_CG Cuts at: 1944 1944 Size: 3749 SacI G_AGCT'C Cuts at: 763 763 Size: 3749 SalI G'TCGA_C Cuts at: 786 786 Size: 3749 SapI GCTCTTCn'nnn_ Cuts at: 737 1772 1982 737 Size: 1035 210 2504 Fragments arranged by size: 2504 1035 210 SbfI CC_TGCA'GG Cuts at: 796 796 Size: 3749 ScaI AGT'ACT Cuts at: 3266 3266 Size: 3749 SfcI C'TryA_G Cuts at: 33 568 643 664 712 792 1477 2470 2661 Size: 535 75 21 48 80 685 993 191 Cuts at: 2661 33 Size: 1121 Fragments arranged by size: 1121 993 685 535 191 80 75 48 21 SfoI GGC'GCC Cuts at: 1181 1429 1181 Size: 248 3501 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3749 SmaI CCC'GGG Cuts at: 771 771 Size: 3749 SmlI C'TyrA_G Cuts at: 904 1819 2311 2573 904 Size: 915 492 262 2080 Fragments arranged by size: 2080 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3749 SphI G_CATG'C Cuts at: 802 1302 802 Size: 500 3249 StyI C'CwwG_G Cuts at: 883 1237 883 Size: 354 3395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 124 1332 1465 1924 2133 124 Size: 1208 133 459 209 1740 Fragments arranged by size: 1740 1208 459 209 133 TatI w'GTAC_w Cuts at: 544 3264 544 Size: 2720 1029 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3266 3298 3266 Size: 32 3717 Tth111I GACn'n_nGTC Cuts at: 1546 1546 Size: 3749 XbaI T'CTAG_A Cuts at: 588 780 588 Size: 192 3557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1244 1244 Size: 3749 XmaI C'CCGG_G Cuts at: 769 769 Size: 3749 XmnI GAAnn'nnTTC Cuts at: 115 115 Size: 3749 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI