(Circular) (Six-base) MAPSORT of: pF1KB6936.seq Check: 2750 from: 1 to: 3800 pF1KB6936 3800 bp With 182 enzymes: SgfI * October 7, 2009 14:23 .. AccI GT'mk_AC Cuts at: 838 838 Size: 3800 Acc65I G'GTAC_C Cuts at: 224 816 224 Size: 592 3208 AclI AA'CG_TT Cuts at: 3640 3640 Size: 3800 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 616 1625 2057 2804 3072 616 Size: 1009 432 747 268 1344 Fragments arranged by size: 1344 1009 747 432 268 AflIII A'CryG_T Cuts at: 1152 2256 3233 1152 Size: 1104 977 1719 Fragments arranged by size: 1719 1104 977 AgeI A'CCGG_T Cuts at: 2188 2188 Size: 3800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 478 478 Size: 3800 AlwNI CAG_nnn'CTG Cuts at: 1105 2672 1105 Size: 1567 2233 ApaI G_GGCC'C Cuts at: 179 653 179 Size: 474 3326 ApaLI G'TGCA_C Cuts at: 2570 2570 Size: 3800 ApoI r'AATT_y Cuts at: 804 3153 3409 804 Size: 2349 256 1195 Fragments arranged by size: 2349 1195 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3800 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3800 AvaI C'yCGr_G Cuts at: 200 323 562 692 820 200 Size: 123 239 130 128 3180 Fragments arranged by size: 3180 239 130 128 123 BamHI G'GATC_C Cuts at: 605 825 605 Size: 220 3580 BanI G'GyrC_C Cuts at: 224 816 1230 1478 1513 224 Size: 592 414 248 35 2511 Fragments arranged by size: 2511 592 414 248 35 BanII G_rGCy'C Cuts at: 179 448 653 814 1844 179 Size: 269 205 161 1030 2135 Fragments arranged by size: 2135 1030 269 205 161 BbeI G_GCGC'C Cuts at: 1234 1482 1234 Size: 248 3552 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 441 475 441 Size: 34 3766 BciVI GTATCCnnnnn_n' Cuts at: 1698 2459 3800 1698 Size: 761 1341 1698 Fragments arranged by size: 1698 1341 761 BclI T'GATC_A Cuts at: 1320 1320 Size: 3800 BglII A'GATC_T Cuts at: 584 1315 3352 584 Size: 731 2037 1032 Fragments arranged by size: 2037 1032 731 BlpI GC'TnA_GC Cuts at: 530 912 530 Size: 382 3418 Bme1580I G_kGCm'C Cuts at: 179 653 1425 1518 2574 179 Size: 474 772 93 1056 1405 Fragments arranged by size: 1405 1056 772 474 93 BmgBI CAC'GTC Cuts at: 665 665 Size: 3800 BmrI ACTGGGnnnn_n' Cuts at: 1012 1093 1428 3572 1012 Size: 81 335 2144 1240 Fragments arranged by size: 2144 1240 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 606 2167 606 Size: 1561 2239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 432 485 777 432 Size: 53 292 3455 Fragments arranged by size: 3455 292 53 Bpu10I CC'TnA_GC Cuts at: 517 722 517 Size: 205 3595 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 976 1855 2347 2645 976 Size: 879 492 298 2131 Fragments arranged by size: 2131 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3504 3504 Size: 3800 BsaAI yAC'GTr Cuts at: 302 414 1153 302 Size: 112 739 2949 Fragments arranged by size: 2949 739 112 BsaBI GATnn'nnATC Cuts at: 6 1314 3351 6 Size: 1308 2037 455 Fragments arranged by size: 2037 1308 455 BsaHI Gr'CG_yC Cuts at: 355 1231 1479 2181 3677 355 Size: 876 248 702 1496 478 Fragments arranged by size: 1496 876 702 478 248 BsaWI w'CCGG_w Cuts at: 989 1510 2188 2462 2609 3020 989 Size: 521 678 274 147 411 1769 Fragments arranged by size: 1769 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 180 1225 1983 2560 3056 180 Size: 1045 758 577 496 924 Fragments arranged by size: 1045 924 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 357 568 357 Size: 211 3589 BsiEI CG_ry'CG Cuts at: 104 233 1342 1388 2596 104 Size: 129 1109 46 1208 1308 Fragments arranged by size: 1308 1208 1109 129 46 BsiHKAI G_wGCw'C Cuts at: 419 482 814 1592 1782 2574 419 Size: 63 332 778 190 792 1645 Fragments arranged by size: 1645 792 778 332 190 63 Bsp1286I G_dGCh'C Cuts at: 179 419 448 482 653 814 1425 1518 1592 Size: 240 29 34 171 161 611 93 74 Cuts at: 1592 1782 1844 2574 179 Size: 190 62 730 1405 Fragments arranged by size: 1405 730 611 240 190 171 161 93 74 62 34 29 BspEI T'CCGG_A Cuts at: 3020 3020 Size: 3800 BspHI T'CATG_A Cuts at: 2976 2976 Size: 3800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 850 1064 1366 1747 850 Size: 214 302 381 2903 Fragments arranged by size: 2903 381 302 214 BsrBI CCG'CTC Cuts at: 50 2092 2146 3630 3795 50 Size: 2042 54 1484 165 55 Fragments arranged by size: 2042 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 214 293 1712 214 Size: 79 1419 2302 Fragments arranged by size: 2302 1419 79 BsrFI r'CCGG_y Cuts at: 1798 1979 2188 1798 Size: 181 209 3410 Fragments arranged by size: 3410 209 181 BssHII G'CGCG_C Cuts at: 631 631 Size: 3800 BssSI C'ACGA_G Cuts at: 2071 2429 2071 Size: 358 3442 BstAPI GCAn_nnn'nTGC Cuts at: 1177 1177 Size: 3800 BstBI TT'CG_AA Cuts at: 2161 2161 Size: 3800 BstYI r'GATC_y Cuts at: 1 584 605 825 1292 1315 1650 1896 2908 Size: 583 21 220 467 23 335 246 1012 Cuts at: 2908 2994 3006 3352 1 Size: 86 12 346 449 Fragments arranged by size: 1012 583 467 449 346 335 246 220 86 23 21 12 Bsu36I CC'TnA_GG Cuts at: 676 676 Size: 3800 BtgI C'CryG_G Cuts at: 236 401 236 Size: 165 3635 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1750 1931 2094 1750 Size: 181 163 3456 Fragments arranged by size: 3456 181 163 BtsI GCAGTG_nn' Cuts at: 168 193 556 770 1043 168 Size: 25 363 214 273 2925 Fragments arranged by size: 2925 363 273 214 25 DraI TTT'AAA Cuts at: 799 799 Size: 3800 DraIII CAC_nnn'GTG Cuts at: 645 645 Size: 3800 DrdI GACnn_nn'nnGTC Cuts at: 360 1506 2364 360 Size: 1146 858 1796 Fragments arranged by size: 1796 1146 858 EaeI y'GGCC_r Cuts at: 1385 1559 1950 1977 3322 1385 Size: 174 391 27 1345 1863 Fragments arranged by size: 1863 1345 391 174 27 EagI C'GGCC_G Cuts at: 1385 1385 Size: 3800 EarI CTCTTCn'nnn_ Cuts at: 1823 2033 1823 Size: 210 3590 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2008 2318 2464 3610 2008 Size: 310 146 1146 2198 Fragments arranged by size: 2198 1146 310 146 EcoICRI GAG'CTC Cuts at: 812 812 Size: 3800 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 432 485 616 777 1625 2057 2804 3072 432 Size: 53 131 161 848 432 747 268 1160 Fragments arranged by size: 1160 848 747 432 268 161 131 53 EcoNI CCTnn'n_nnAGG Cuts at: 503 790 503 Size: 287 3513 EcoO109I rG'GnC_Cy Cuts at: 175 176 566 649 726 939 175 Size: 1 390 83 77 213 3036 Fragments arranged by size: 3036 390 213 83 77 1 EcoRI G'AATT_C Cuts at: 804 804 Size: 3800 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1819 1819 Size: 3800 FspI TGC'GCA Cuts at: 1581 1581 Size: 3800 HaeII r_GCGC'y Cuts at: 537 1234 1482 2504 3483 537 Size: 697 248 1022 979 854 Fragments arranged by size: 1022 979 854 697 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1642 1672 1674 1704 1642 Size: 30 2 30 3738 Fragments arranged by size: 3738 30 30 2 HincII GTy'rAC Cuts at: 839 839 Size: 3800 KasI G'GCGC_C Cuts at: 1230 1478 1230 Size: 248 3552 KpnI G_GTAC'C Cuts at: 228 820 228 Size: 592 3208 MluI A'CGCG_T Cuts at: 3233 3233 Size: 3800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2471 2655 2471 Size: 184 3616 MscI TGG'CCA Cuts at: 1561 1561 Size: 3800 MslI CAynn'nnrTG Cuts at: 1789 1916 1789 Size: 127 3673 MspA1I CmG'CkG Cuts at: 149 335 371 393 911 1225 1585 2598 2843 Size: 186 36 22 518 314 360 1013 245 Cuts at: 2843 149 Size: 1106 Fragments arranged by size: 1106 1013 518 360 314 245 186 36 22 NaeI GCC'GGC Cuts at: 1981 1981 Size: 3800 NarI GG'CG_CC Cuts at: 1231 1479 1231 Size: 248 3552 NcoI C'CATG_G Cuts at: 401 401 Size: 3800 NgoMIV G'CCGG_C Cuts at: 1979 1979 Size: 3800 NspI r_CATG'y Cuts at: 853 1353 2260 853 Size: 500 907 2393 Fragments arranged by size: 2393 907 500 PasI CC'CwG_GG Cuts at: 440 440 Size: 3800 PciI A'CATG_T Cuts at: 2256 2256 Size: 3800 PflMI CCAn_nnn'nTGG Cuts at: 645 645 Size: 3800 PfoI T'CCnGG_A Cuts at: 247 247 Size: 3800 PmeI GTTT'AAAC Cuts at: 799 799 Size: 3800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2939 2971 2939 Size: 32 3768 PpuMI rG'GwC_Cy Cuts at: 566 726 566 Size: 160 3640 PshAI GACnn'nnGTC Cuts at: 758 758 Size: 3800 PspOMI G'GGCC_C Cuts at: 175 649 175 Size: 474 3326 PstI C_TGCA'G Cuts at: 163 350 847 1532 163 Size: 187 497 685 2431 Fragments arranged by size: 2431 685 497 187 PvuI CG_AT'CG Cuts at: 104 233 104 Size: 129 3671 PvuII CAG'CTG Cuts at: 149 335 371 393 1225 1585 149 Size: 186 36 22 832 360 2364 Fragments arranged by size: 2364 832 360 186 36 22 RsrII CG'GwC_CG Cuts at: 1995 1995 Size: 3800 SacI G_AGCT'C Cuts at: 814 814 Size: 3800 SalI G'TCGA_C Cuts at: 837 837 Size: 3800 SapI GCTCTTCn'nnn_ Cuts at: 1823 2033 1823 Size: 210 3590 SbfI CC_TGCA'GG Cuts at: 847 847 Size: 3800 ScaI AGT'ACT Cuts at: 601 3317 601 Size: 2716 1084 SfcI C'TryA_G Cuts at: 33 159 346 541 843 1528 2521 2712 33 Size: 126 187 195 302 685 993 191 1121 Fragments arranged by size: 1121 993 685 302 195 191 187 126 SfoI GGC'GCC Cuts at: 1232 1480 1232 Size: 248 3552 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3800 SmaI CCC'GGG Cuts at: 202 325 564 822 202 Size: 123 239 258 3180 Fragments arranged by size: 3180 258 239 123 SmlI C'TyrA_G Cuts at: 955 1870 2362 2624 955 Size: 915 492 262 2131 Fragments arranged by size: 2131 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3800 SphI G_CATG'C Cuts at: 853 1353 853 Size: 500 3300 StyI C'CwwG_G Cuts at: 401 934 1288 401 Size: 533 354 2913 Fragments arranged by size: 2913 533 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1383 1516 1975 2184 1383 Size: 133 459 209 2999 Fragments arranged by size: 2999 459 209 133 TatI w'GTAC_w Cuts at: 599 3315 599 Size: 2716 1084 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3317 3349 3317 Size: 32 3768 Tth111I GACn'n_nGTC Cuts at: 1597 1597 Size: 3800 XbaI T'CTAG_A Cuts at: 831 831 Size: 3800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 783 1295 783 Size: 512 3288 XmaI C'CCGG_G Cuts at: 200 323 562 820 200 Size: 123 239 258 3180 Fragments arranged by size: 3180 258 239 123 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI