(Circular) (Six-base) MAPSORT of: pF1KE4526.seq Check: 7232 from: 1 to: 4835 pF1KE4526 4835 bp With 182 enzymes: SgfI * March 8, 2012 13:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 246 246 Size: 4835 AccI GT'mk_AC Cuts at: 1873 1873 Size: 4835 Acc65I G'GTAC_C Cuts at: 1034 1851 1034 Size: 817 4018 AclI AA'CG_TT Cuts at: 4675 4675 Size: 4835 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2660 3092 3839 4107 2660 Size: 432 747 268 3388 Fragments arranged by size: 3388 747 432 268 AfeI AGC'GCT Cuts at: 310 1588 310 Size: 1278 3557 AflIII A'CryG_T Cuts at: 2187 3291 4268 2187 Size: 1104 977 2754 Fragments arranged by size: 2754 1104 977 AgeI A'CCGG_T Cuts at: 3223 3223 Size: 4835 AhdI GACnn_n'nnGTC Cuts at: 687 687 Size: 4835 AleI CACnn'nnGTG Cuts at: 1258 1258 Size: 4835 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1827 1827 Size: 4835 AlwNI CAG_nnn'CTG Cuts at: 1547 1565 1617 2140 3707 1547 Size: 18 52 523 1567 2675 Fragments arranged by size: 2675 1567 523 52 18 ApaI G_GGCC'C Cuts at: 144 534 144 Size: 390 4445 ApaLI G'TGCA_C Cuts at: 175 1711 3605 175 Size: 1536 1894 1405 Fragments arranged by size: 1894 1536 1405 ApoI r'AATT_y Cuts at: 1839 4188 4444 1839 Size: 2349 256 2230 Fragments arranged by size: 2349 2230 256 AscI GG'CGCG_CC Cuts at: 344 344 Size: 4835 AseI AT'TA_AT Cuts at: 20 20 Size: 4835 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4835 AvaI C'yCGr_G Cuts at: 119 196 276 326 527 797 838 871 991 Size: 77 80 50 201 270 41 33 120 Cuts at: 991 1060 1855 119 Size: 69 795 3099 Fragments arranged by size: 3099 795 270 201 120 80 77 69 50 41 33 BamHI G'GATC_C Cuts at: 1860 1860 Size: 4835 BanI G'GyrC_C Cuts at: 154 396 455 477 651 952 1034 1851 2265 Size: 242 59 22 174 301 82 817 414 Cuts at: 2265 2513 2548 154 Size: 248 35 2441 Fragments arranged by size: 2441 817 414 301 248 242 174 82 59 35 22 BanII G_rGCy'C Cuts at: 144 208 283 382 430 534 568 1272 1624 Size: 64 75 99 48 104 34 704 352 Cuts at: 1624 1849 2879 144 Size: 225 1030 2100 Fragments arranged by size: 2100 1030 704 352 225 104 99 75 64 48 34 BbeI G_GCGC'C Cuts at: 158 400 655 2269 2517 158 Size: 242 255 1614 248 2476 Fragments arranged by size: 2476 1614 255 248 242 BbvCI CC'TCA_GC Cuts at: 305 1168 305 Size: 863 3972 BciVI GTATCCnnnnn_n' Cuts at: 2733 3494 4835 2733 Size: 761 1341 2733 Fragments arranged by size: 2733 1341 761 BclI T'GATC_A Cuts at: 2355 2355 Size: 4835 BfrBI ATG'CAT Cuts at: 1594 1594 Size: 4835 BglI GCCn_nnn'nGGC Cuts at: 1336 1336 Size: 4835 BglII A'GATC_T Cuts at: 2350 4387 2350 Size: 2037 2798 BlpI GC'TnA_GC Cuts at: 1947 1947 Size: 4835 Bme1580I G_kGCm'C Cuts at: 144 179 482 534 955 1399 1715 2460 2553 Size: 35 303 52 421 444 316 745 93 Cuts at: 2553 3609 144 Size: 1056 1370 Fragments arranged by size: 1370 1056 745 444 421 316 303 93 52 35 BmgBI CAC'GTC Cuts at: 1683 1683 Size: 4835 BmrI ACTGGGnnnn_n' Cuts at: 2047 2128 2463 4607 2047 Size: 81 335 2144 2275 Fragments arranged by size: 2275 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 339 3202 339 Size: 2863 1972 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1108 1573 1108 Size: 465 4370 Bpu10I CC'TnA_GC Cuts at: 305 1168 1618 305 Size: 863 450 3522 Fragments arranged by size: 3522 863 450 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1567 1690 2011 2890 3382 3680 1567 Size: 123 321 879 492 298 2722 Fragments arranged by size: 2722 879 492 321 298 123 BsaI GGTCTCn'nnnn_ Cuts at: 585 1010 1216 4539 585 Size: 425 206 3323 881 Fragments arranged by size: 3323 881 425 206 BsaAI yAC'GTr Cuts at: 291 885 1716 2188 291 Size: 594 831 472 2938 Fragments arranged by size: 2938 831 594 472 BsaBI GATnn'nnATC Cuts at: 6 2349 4386 6 Size: 2343 2037 455 Fragments arranged by size: 2343 2037 455 BsaHI Gr'CG_yC Cuts at: 155 387 397 652 2266 2514 3216 4712 155 Size: 232 10 255 1614 248 702 1496 278 Fragments arranged by size: 1614 1496 702 278 255 248 232 10 BsaWI w'CCGG_w Cuts at: 2024 2545 3223 3497 3644 4055 2024 Size: 521 678 274 147 411 2804 Fragments arranged by size: 2804 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 550 580 1264 1291 1294 1321 550 Size: 30 684 27 3 27 4064 Fragments arranged by size: 4064 684 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 564 826 1315 564 Size: 262 489 4084 Fragments arranged by size: 4084 489 262 BseYI C'CCAG_C Cuts at: 135 356 548 1376 2260 3018 3595 4091 135 Size: 221 192 828 884 758 577 496 879 Fragments arranged by size: 884 879 828 758 577 496 221 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1282 1705 1282 Size: 423 4412 BsiEI CG_ry'CG Cuts at: 104 299 761 1206 2377 2423 3631 104 Size: 195 462 445 1171 46 1208 1308 Fragments arranged by size: 1308 1208 1171 462 445 195 46 BsiHKAI G_wGCw'C Cuts at: 179 1715 1849 2627 2817 3609 179 Size: 1536 134 778 190 792 1405 Fragments arranged by size: 1536 1405 792 778 190 134 BsiWI C'GTAC_G Cuts at: 1067 1067 Size: 4835 BsmBI CGTCTCn'nnnn_ Cuts at: 549 549 Size: 4835 Bsp1286I G_dGCh'C Cuts at: 144 179 208 283 382 430 482 534 568 Size: 35 29 75 99 48 52 52 34 Cuts at: 568 955 1272 1399 1624 1715 1849 2460 2553 Size: 387 317 127 225 91 134 611 93 Cuts at: 2553 2627 2817 2879 3609 144 Size: 74 190 62 730 1370 Fragments arranged by size: 1370 730 611 387 317 225 190 134 127 99 93 91 75 74 62 52 52 48 35 34 29 BspEI T'CCGG_A Cuts at: 4055 4055 Size: 4835 BspHI T'CATG_A Cuts at: 4011 4011 Size: 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 246 1109 1885 2099 2401 2782 246 Size: 863 776 214 302 381 2299 Fragments arranged by size: 2299 863 776 381 302 214 BsrBI CCG'CTC Cuts at: 50 1143 3127 3181 4665 4830 50 Size: 1093 1984 54 1484 165 55 Fragments arranged by size: 1984 1484 1093 165 55 54 BsrDI GCAATG_nn' Cuts at: 2747 2747 Size: 4835 BsrFI r'CCGG_y Cuts at: 128 294 340 722 1104 1187 1418 2833 3014 Size: 166 46 382 382 83 231 1415 181 Cuts at: 3014 3223 128 Size: 209 1740 Fragments arranged by size: 1740 1415 382 382 231 209 181 166 83 46 BsrGI T'GTAC_A Cuts at: 260 941 260 Size: 681 4154 BssHII G'CGCG_C Cuts at: 344 802 1080 1082 1151 344 Size: 458 278 2 69 4028 Fragments arranged by size: 4028 458 278 69 2 BssSI C'ACGA_G Cuts at: 3106 3464 3106 Size: 358 4477 BstAPI GCAn_nnn'nTGC Cuts at: 2212 2212 Size: 4835 BstBI TT'CG_AA Cuts at: 3196 3196 Size: 4835 BstXI CCAn_nnnn'nTGG Cuts at: 114 1761 114 Size: 1647 3188 BstYI r'GATC_y Cuts at: 1 1860 2327 2350 2685 2931 3943 4029 4041 Size: 1859 467 23 335 246 1012 86 12 Cuts at: 4041 4387 1 Size: 346 449 Fragments arranged by size: 1859 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 851 1334 851 Size: 483 4352 BtgI C'CryG_G Cuts at: 107 372 728 761 1008 1206 1423 107 Size: 265 356 33 247 198 217 3519 Fragments arranged by size: 3519 356 265 247 217 198 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2785 2966 3129 2785 Size: 181 163 4491 Fragments arranged by size: 4491 181 163 BtsI GCAGTG_nn' Cuts at: 2078 2078 Size: 4835 DraI TTT'AAA Cuts at: 1834 1834 Size: 4835 DrdI GACnn_nn'nnGTC Cuts at: 2541 3399 2541 Size: 858 3977 EaeI y'GGCC_r Cuts at: 296 338 444 471 500 758 1203 1313 1420 Size: 42 106 27 29 258 445 110 107 Cuts at: 1420 1523 2420 2594 2985 3012 4357 296 Size: 103 897 174 391 27 1345 774 Fragments arranged by size: 1345 897 774 445 391 258 174 110 107 106 103 42 29 27 27 EagI C'GGCC_G Cuts at: 296 758 1203 2420 296 Size: 462 445 1217 2711 Fragments arranged by size: 2711 1217 462 445 EarI CTCTTCn'nnn_ Cuts at: 824 2858 3068 824 Size: 2034 210 2591 Fragments arranged by size: 2591 2034 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 558 1028 3043 3353 3499 4645 558 Size: 470 2015 310 146 1146 748 Fragments arranged by size: 2015 1146 748 470 310 146 EcoICRI GAG'CTC Cuts at: 1847 1847 Size: 4835 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1108 1573 2660 3092 3839 4107 1108 Size: 465 1087 432 747 268 1836 Fragments arranged by size: 1836 1087 747 465 432 268 EcoO109I rG'GnC_Cy Cuts at: 140 141 878 1614 1974 140 Size: 1 737 736 360 3001 Fragments arranged by size: 3001 737 736 360 1 EcoRI G'AATT_C Cuts at: 1839 1839 Size: 4835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2854 2854 Size: 4835 FspI TGC'GCA Cuts at: 1111 2616 1111 Size: 1505 3330 HaeII r_GCGC'y Cuts at: 158 312 400 655 1590 2269 2517 3539 4518 Size: 154 88 255 935 679 248 1022 979 Cuts at: 4518 158 Size: 475 Fragments arranged by size: 1022 979 935 679 475 255 248 154 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 673 705 2677 2707 2709 2739 673 Size: 32 1972 30 2 30 2769 Fragments arranged by size: 2769 1972 32 30 30 2 HincII GTy'rAC Cuts at: 1874 1874 Size: 4835 KasI G'GCGC_C Cuts at: 154 396 651 2265 2513 154 Size: 242 255 1614 248 2476 Fragments arranged by size: 2476 1614 255 248 242 KpnI G_GTAC'C Cuts at: 1038 1855 1038 Size: 817 4018 MluI A'CGCG_T Cuts at: 4268 4268 Size: 4835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1142 3506 3690 1142 Size: 2364 184 2287 Fragments arranged by size: 2364 2287 184 MscI TGG'CCA Cuts at: 473 1315 2596 473 Size: 842 1281 2712 Fragments arranged by size: 2712 1281 842 MslI CAynn'nnrTG Cuts at: 520 1258 2824 2951 520 Size: 738 1566 127 2404 Fragments arranged by size: 2404 1566 738 127 MspA1I CmG'CkG Cuts at: 135 221 231 363 730 1208 1448 1946 2260 Size: 86 10 132 367 478 240 498 314 Cuts at: 2260 2620 3633 3878 135 Size: 360 1013 245 1092 Fragments arranged by size: 1092 1013 498 478 367 360 314 245 240 132 86 10 NaeI GCC'GGC Cuts at: 130 296 342 3016 130 Size: 166 46 2674 1949 Fragments arranged by size: 2674 1949 166 46 NarI GG'CG_CC Cuts at: 155 397 652 2266 2514 155 Size: 242 255 1614 248 2476 Fragments arranged by size: 2476 1614 255 248 242 NcoI C'CATG_G Cuts at: 107 1008 107 Size: 901 3934 NgoMIV G'CCGG_C Cuts at: 128 294 340 3014 128 Size: 166 46 2674 1949 Fragments arranged by size: 2674 1949 166 46 NsiI A_TGCA'T Cuts at: 1596 1596 Size: 4835 NspI r_CATG'y Cuts at: 1598 1888 2388 3295 1598 Size: 290 500 907 3138 Fragments arranged by size: 3138 907 500 290 PasI CC'CwG_GG Cuts at: 1611 1611 Size: 4835 PciI A'CATG_T Cuts at: 3291 3291 Size: 4835 PflMI CCAn_nnn'nTGG Cuts at: 1470 1470 Size: 4835 PmeI GTTT'AAAC Cuts at: 1834 1834 Size: 4835 PmlI CAC'GTG Cuts at: 1716 1716 Size: 4835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 3974 Size: 32 4803 PshAI GACnn'nnGTC Cuts at: 986 986 Size: 4835 PspOMI G'GGCC_C Cuts at: 140 530 140 Size: 390 4445 PstI C_TGCA'G Cuts at: 1882 2567 1882 Size: 685 4150 PvuI CG_AT'CG Cuts at: 104 104 Size: 4835 PvuII CAG'CTG Cuts at: 135 231 1448 2260 2620 135 Size: 96 1217 812 360 2350 Fragments arranged by size: 2350 1217 812 360 96 RsrII CG'GwC_CG Cuts at: 3030 3030 Size: 4835 SacI G_AGCT'C Cuts at: 1849 1849 Size: 4835 SacII CC_GC'GG Cuts at: 731 1209 731 Size: 478 4357 SalI G'TCGA_C Cuts at: 1872 1872 Size: 4835 SapI GCTCTTCn'nnn_ Cuts at: 824 2858 3068 824 Size: 2034 210 2591 Fragments arranged by size: 2591 2034 210 SbfI CC_TGCA'GG Cuts at: 1882 1882 Size: 4835 ScaI AGT'ACT Cuts at: 4352 4352 Size: 4835 SfcI C'TryA_G Cuts at: 33 1878 2563 3556 3747 33 Size: 1845 685 993 191 1121 Fragments arranged by size: 1845 1121 993 685 191 SfoI GGC'GCC Cuts at: 156 398 653 2267 2515 156 Size: 242 255 1614 248 2476 Fragments arranged by size: 2476 1614 255 248 242 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4835 SmaI CCC'GGG Cuts at: 198 328 529 799 1857 198 Size: 130 201 270 1058 3176 Fragments arranged by size: 3176 1058 270 201 130 SmlI C'TyrA_G Cuts at: 1582 1705 1990 2905 3397 3659 1582 Size: 123 285 915 492 262 2758 Fragments arranged by size: 2758 915 492 285 262 123 SpeI A'CTAG_T Cuts at: 67 67 Size: 4835 SphI G_CATG'C Cuts at: 1598 1888 2388 1598 Size: 290 500 4045 Fragments arranged by size: 4045 500 290 SrfI GCCC'GGGC Cuts at: 198 529 198 Size: 331 4504 StuI AGG'CCT Cuts at: 973 973 Size: 4835 StyI C'CwwG_G Cuts at: 107 1008 1969 2323 107 Size: 901 961 354 2619 Fragments arranged by size: 2619 961 901 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 473 567 2418 2551 3010 3219 473 Size: 94 1851 133 459 209 2089 Fragments arranged by size: 2089 1851 459 209 133 94 TatI w'GTAC_w Cuts at: 260 716 941 1277 4350 260 Size: 456 225 336 3073 745 Fragments arranged by size: 3073 745 456 336 225 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 550 582 1264 1291 1296 1323 4352 4384 550 Size: 32 682 27 5 27 3029 32 1001 Fragments arranged by size: 3029 1001 682 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1222 2632 1222 Size: 1410 3425 XbaI T'CTAG_A Cuts at: 1866 1866 Size: 4835 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1028 1430 1548 2330 1028 Size: 402 118 782 3533 Fragments arranged by size: 3533 782 402 118 XmaI C'CCGG_G Cuts at: 196 326 527 797 1855 196 Size: 130 201 270 1058 3176 Fragments arranged by size: 3176 1058 270 201 130 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AloI AvrII BaeI BbsI BcgI BmtI BsmI BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PfoI PpuMI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI