Restriction map for pF1KB6103
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstEII      BstPI       BstUI       
BstXI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraII       DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HphI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI         1103  GTATAC               GT^[AC][GT]AC
AciI         1098  CCGC                 CCGC(-3/-1)
AclI          400  AACGTT               AA^CGTT
AfaI         1091  GTAC                 GT^AC
AlwNI         656  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1598  GGGCCC               GGGCC^C
Asp700I       970  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII        1620  GGACC                G^G[AT]CC
BamHI        1408  GGATCC               G^GATCC
BanII        1598  GGGCCC               G[GA]GC[CT]^C
BbsI           22  GAAGAC               GAAGAC(2/6)
BcgI          543  CGAATATTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1596  CCGGG                CC^[GC]GG
BmyI         1598  GGGCCC               G[AGT]GC[ACT]^C
BpmI           34  CTGGAG               CTGGAG(16/14)
BpuAI          22  GAAGAC               GAAGAC(2/6)
BsaI          521  GGTCTC               GGTCTC(1/5)
BsiYI        1176  CCACAATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1176  CCACAATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         522  GTCTC                GTCTC(1/5)
BsmFI        1619  GGGAC                GGGAC(10/14)
BsmI         1567  GCATTC               GAATGC(1/-1)
Bsp1286I     1598  GGGCCC               G[AGT]GC[ACT]^C
Bst1107I     1103  GTATAC               GTA^TAC
BstAPI        807  GCAAACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         977  TTCGAA               TT^CGAA
BstYI        1408  GGATCC               [GA]^GATC[CT]
BstZ17I      1103  GTATAC               GTA^TAC
CfoI          118  GCGC                 GCG^C
DraI         1138  TTTAAA               TTT^AAA
DraIII         43  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          181  GACCAGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1056  GAATTC               G^AATTC
HapII        1596  CCGG                 C^CGG
HhaI          118  GCGC                 GCG^C
HinP1I        118  GCGC                 G^CGC
HindIII      1627  AAGCTT               A^AGCTT
HpaI         1605  GTTAAC               GTT^AAC
HpaII        1596  CCGG                 C^CGG
MflI         1408  GGATCC               [GA]^GATC[CT]
MslI         1276  CACCTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1495  CAGCTG               C[AC]G^C[GT]G
MspI         1596  CCGG                 C^CGG
NciI         1596  CCGGG                CC^[GC]GG
NdeI         1084  CATATG               CA^TATG
NspV          977  TTCGAA               TT^CGAA
PflMI        1176  CCACAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1314  GAGTC                GAGTC(4/5)
Psp1406I      400  AACGTT               AA^CGTT
PstI          775  CTGCAG               CTGCA^G
PvuII        1495  CAGCTG               CAG^CTG
RsaI         1091  GTAC                 GT^AC
SapI         1209  GAAGAGC              GCTCTTC(1/4)
SfuI          977  TTCGAA               TT^CGAA
Tsp45I        710  GTGAC                ^GT[GC]AC
Van91I       1176  CCACAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI           78  TCTAGA               T^CTAGA
XcmI         1215  CCAGCAAATCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1408  GGATCC               [GA]^GATC[CT]
XmnI          970  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AseI          507  ATTAAT               AT^TAAT
             1671  ATTAAT              
AsnI          507  ATTAAT               AT^TAAT
             1671  ATTAAT              
BsaJI         517  CCAAGG               C^C[ACGT][ACGT]GG
              918  CCTTGG              
Cac8I         114  GCGAGC               GC[ACGT]^[ACGT]GC
             1214  GCCAGC              
EcoT14I       517  CCAAGG               C^C[AT][AT]GG
              918  CCTTGG              
MaeII         401  ACGT                 A^CGT
             1007  ACGT                
MaeIII        710  GTGAC                ^GT[ACGT]AC
              818  GTAAC               
PshBI         507  ATTAAT               AT^TAAT
             1671  ATTAAT              
SfcI          260  CTACAG               C^T[GA][CT]AG
              775  CTGCAG              
SspI          545  AATATT               AAT^ATT
              725  AATATT              
StyI          517  CCAAGG               C^C[AT][AT]GG
              918  CCTTGG              
TaqI          978  TCGA                 T^CGA
             1079  TCGA                
TthHB8I       978  TCGA                 T^CGA
             1079  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbvI        
BfaI        BseRI       BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HincII      
HindII      HinfI       ItaI        Ksp632I     MaeI        
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        Tru9I       
TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com