Restriction map for pF1KB5919
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaHI       BsaI        BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Cac8I       CelII       ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MslI        MspA1I      MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tth111I     TthHB8I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          994  GTAGAC               GT^[AC][GT]AC
AccII         533  CGCG                 CG^CG
AfaI           30  GTAC                 GT^AC
AgeI          409  ACCGGT               A^CCGGT
AlwNI         703  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           98  GGGCCC               GGGCC^C
BalI          129  TGGCCA               TGG^CCA
BamHI         807  GGATCC               G^GATCC
BanI         1315  GGTGCC               G^G[CT][GA]CC
BanII          98  GGGCCC               G[GA]GC[CT]^C
BglII        1059  AGATCT               A^GATCT
BsaAI         310  TACGTG               [CT]AC^GT[GA]
BsaBI         716  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         409  ACCGGT               [AT]^CCGG[AT]
BsmFI         637  GTCCC                GGGAC(10/14)
BsmI         1410  GAATGC               GAATGC(1/-1)
BspMI         193  GCAGGT               ACCTGC(4/8)
BstAPI       1006  GCACAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         533  CGCG                 CG^CG
BstXI         245  CCAGACACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1201  GCGC                 GCG^C
DrdI          929  GACATGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          129  TGGCCA               [CT]^GGCC[GA]
EarI         1482  CTCTTC               CTCTTC(1/4)
EcoNI         822  CCTGAGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         451  GAATTC               G^AATTC
HhaI         1201  GCGC                 GCG^C
HinP1I       1201  GCGC                 G^CGC
HincII         75  GTCAAC               GT[CT]^[GA]AC
HindII         75  GTCAAC               GT[CT]^[GA]AC
HindIII       862  AAGCTT               A^AGCTT
Ksp632I      1482  CTCTTC               CTCTTC(1/4)
MaeII         311  ACGT                 A^CGT
MamI          716  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         129  TGGCCA               TGG^CCA
MscI          129  TGGCCA               TGG^CCA
MvnI          533  CGCG                 CG^CG
PflMI        1329  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         409  ACCGGT               A^CCGGT
PpuMI         635  AGGTCCC              [GA]G^G[AT]CC[CT]
RsaI           30  GTAC                 GT^AC
SexAI         795  ACCAGGT              A^CC[AT]GGT
SmlI          180  CTTGAG               C^T[CT][GA]AG
Van91I       1329  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1165  TCTAGA               T^CTAGA
XcmI          954  CCAAAATGACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AocI          822  CCTGAGG              CC^T[ACGT]AGG
             1420  CCTAAGG             
Asp700I       542  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1207  GAAGCCATTC          
AvaII         636  GGTCC                G^G[AT]CC
              672  GGACC               
BbsI         1150  GAAGAC               GAAGAC(2/6)
             1462  GAAGAC              
BbvCI         172  GCTGAGG              CCTCAGC(-5/-2)
              316  GCTGAGG             
BbvI         1066  GCTGC                GCAGC(8/12)
             1182  GCTGC               
BfaI           34  CTAG                 C^TAG
             1166  CTAG                
BmyI           98  GGGCCC               G[AGT]GC[ACT]^C
             1316  GTGCCC              
BpuAI        1150  GAAGAC               GAAGAC(2/6)
             1462  GAAGAC              
Bsp1286I       98  GGGCCC               G[AGT]GC[ACT]^C
             1316  GTGCCC              
BsrFI         409  ACCGGT               [GA]^CCGG[CT]
             1185  GCCGGT              
Bsu36I        822  CCTGAGG              CC^T[ACGT]AGG
             1420  CCTAAGG             
Cfr10I        409  ACCGGT               [GA]^CCGG[CT]
             1185  GCCGGT              
Eco81I        822  CCTGAGG              CC^T[ACGT]AGG
             1420  CCTAAGG             
HapII         410  CCGG                 C^CGG
             1186  CCGG                
HpaII         410  CCGG                 C^CGG
             1186  CCGG                
MaeI           34  CTAG                 C^TAG
             1166  CTAG                
MspI          410  CCGG                 C^CGG
             1186  CCGG                
SfaNI         373  GATGC                GCATC(5/9)
             1495  GATGC               
TseI         1066  GCTGC                G^C[AT]GC
             1182  GCTGC               
XmnI          542  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1207  GAAGCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfcI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       XhoII       
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