Restriction map for pF1KB5919
 (Linear) (Six-base) MAPSORT of: KIBB5919.seq  Check: 5391  from: 1  to: 1515



KIBB5919.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     184    1515 
   Size:        184    1331
 
AccI GT'mk_AC

Cuts at:      0     995    1515 
   Size:        995     520
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730    1515 
   Size:        730     785
 
AgeI A'CCGG_T

Cuts at:      0     409    1515 
   Size:        409    1106
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29      61    1515 
   Size:         29      32    1454

  Fragments arranged by size: 

                1454      32      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     708    1515 
   Size:        708     807
 
ApaI G_GGCC'C

Cuts at:      0     102    1515 
   Size:        102    1413
 
ApoI r'AATT_y

Cuts at:      0     451     562    1225    1515 
   Size:        451     111     663     290

  Fragments arranged by size: 

                 663     451     290     111
 
BamHI G'GATC_C

Cuts at:      0     807    1515 
   Size:        807     708
 
BanI G'GyrC_C

Cuts at:      0    1315    1515 
   Size:       1315     200
 
BanII G_rGCy'C

Cuts at:      0     102    1515 
   Size:        102    1413
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1157    1469    1515 
   Size:       1157     312      46

  Fragments arranged by size: 

                1157     312      46
 
BbvCI CC'TCA_GC

Cuts at:      0     173     317    1515 
   Size:        173     144    1198

  Fragments arranged by size: 

                1198     173     144
 
BglII A'GATC_T

Cuts at:      0    1059    1515 
   Size:       1059     456
 
Bme1580I G_kGCm'C

Cuts at:      0     102    1320    1515 
   Size:        102    1218     195

  Fragments arranged by size: 

                1218     195     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     108    1515 
   Size:        108    1407
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     755    1054    1515 
   Size:        258     497     299     461

  Fragments arranged by size: 

                 497     461     299     258
 
Bpu10I CC'TnA_GC

Cuts at:      0     173     317    1515 
   Size:        173     144    1198

  Fragments arranged by size: 

                1198     173     144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201    1515 
   Size:        201    1314
 
BsaAI yAC'GTr

Cuts at:      0     312    1515 
   Size:        312    1203
 
BsaBI GATnn'nnATC

Cuts at:      0     720    1515 
   Size:        720     795
 
BsaWI w'CCGG_w

Cuts at:      0     409    1515 
   Size:        409    1106
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      29      59     467     497     679     709    1515 
   Size:         29      30     408      30     182      30     806

  Fragments arranged by size: 

                 806     408     182      30      30      30      29
 
BseYI C'CCAG_C

Cuts at:      0     609     639    1515 
   Size:        609      30     876

  Fragments arranged by size: 

                 876     609      30
 
BsmI GAATG_Cn'

Cuts at:      0    1416    1515 
   Size:       1416      99
 
Bsp1286I G_dGCh'C

Cuts at:      0     102    1320    1515 
   Size:        102    1218     195

  Fragments arranged by size: 

                1218     195     102
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     184    1515 
   Size:        184    1331
 
BsrFI r'CCGG_y

Cuts at:      0     409    1185    1515 
   Size:        409     776     330

  Fragments arranged by size: 

                 776     409     330
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1012    1515 
   Size:       1012     503
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     252    1515 
   Size:        252    1263
 
BstYI r'GATC_y

Cuts at:      0     629     721     807     897    1059    1515 
   Size:        629      92      86      90     162     456

  Fragments arranged by size: 

                 629     456     162      92      90      86
 
Bsu36I CC'TnA_GG

Cuts at:      0     823    1421    1515 
   Size:        823     598      94

  Fragments arranged by size: 

                 823     598      94
 
BtgI C'CryG_G

Cuts at:      0      59     343     668    1515 
   Size:         59     284     325     847

  Fragments arranged by size: 

                 847     325     284      59
 
BtsI GCAGTG_nn'

Cuts at:      0     785    1444    1515 
   Size:        785     659      71

  Fragments arranged by size: 

                 785     659      71
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     935    1515 
   Size:        935     580
 
EaeI y'GGCC_r

Cuts at:      0     129    1515 
   Size:        129    1386
 
EarI CTCTTCn'nnn_

Cuts at:      0    1488    1515 
   Size:       1488      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     730     755    1054    1515 
   Size:        258     472      25     299     461

  Fragments arranged by size: 

                 472     461     299     258      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     826    1515 
   Size:        826     689
 
EcoO109I rG'GnC_Cy

Cuts at:      0      98      99     636    1515 
   Size:         98       1     537     879

  Fragments arranged by size: 

                 879     537      98       1
 
EcoRI G'AATT_C

Cuts at:      0     451    1515 
   Size:        451    1064
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107     512     544     679     711     952     959 
   Size:         75      32     405      32     135      32     241       7

Cuts at:    959     984     991    1234    1266    1323    1355    1515 
   Size:         25       7     243      32      57      32     160

  Fragments arranged by size: 

                 405     243     241     160     135      75      57      32
                  32      32      32      32      25       7       7
 
HincII GTy'rAC

Cuts at:      0      77    1515 
   Size:         77    1438
 
HindIII A'AGCT_T

Cuts at:      0     862    1515 
   Size:        862     653
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1379    1391    1432    1515 
   Size:       1379      12      41      83

  Fragments arranged by size: 

                1379      83      41      12
 
MscI TGG'CCA

Cuts at:      0     131    1515 
   Size:        131    1384
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1335    1515 
   Size:       1335     180
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      29      61     331     363    1515 
   Size:         29      32     270      32    1152

  Fragments arranged by size: 

                1152     270      32      32      29
 
PpuMI rG'GwC_Cy

Cuts at:      0     636    1515 
   Size:        636     879
 
PspOMI G'GGCC_C

Cuts at:      0      98    1515 
   Size:         98    1417
 
SexAI A'CCwGG_T

Cuts at:      0     795    1515 
   Size:        795     720
 
SfcI C'TryA_G

Cuts at:      0     331     492    1347    1515 
   Size:        331     161     855     168

  Fragments arranged by size: 

                 855     331     168     161
 
SmlI C'TyrA_G

Cuts at:      0     180    1515 
   Size:        180    1335
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     739     771    1515 
   Size:        739      32     744

  Fragments arranged by size: 

                 744     739      32
 
XbaI T'CTAG_A

Cuts at:      0    1165    1515 
   Size:       1165     350
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     961    1515 
   Size:        961     554
 
XmnI GAAnn'nnTTC

Cuts at:      0     546    1211    1515 
   Size:        546     665     304

  Fragments arranged by size: 

                 665     546     304
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AgeI     AloI    AlwNI     ApaI     ApoI
    BamHI     BanI    BanII     BbsI    BbvCI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
     BsmI Bsp1286I    BspMI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    Hin4I   HincII  HindIII     MmeI     MscI    PflMI     PpiI
    PpuMI   PspOMI    SexAI     SfcI     SmlI     TstI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI     BsaI    BsaHI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XhoI     XmaI     ZraI
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