Restriction map for pF1KB6957
 (Linear) (Six-base) MAPSORT of: KIBB6957.seq  Check: 3760  from: 1  to: 639



KIBB6957.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

Acc65I G'GTAC_C

Cuts at:      0     457     639 
   Size:        457     182
 
AfeI AGC'GCT

Cuts at:      0      67     639 
   Size:         67     572
 
AleI CACnn'nnGTG

Cuts at:      0     399     639 
   Size:        399     240
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     226     639 
   Size:        226     413
 
AlwNI CAG_nnn'CTG

Cuts at:      0     245     639 
   Size:        245     394
 
ApaI G_GGCC'C

Cuts at:      0     147     154     639 
   Size:        147       7     485

  Fragments arranged by size: 

                 485     147       7
 
AvaI C'yCGr_G

Cuts at:      0     251     547     639 
   Size:        251     296      92

  Fragments arranged by size: 

                 296     251      92
 
BanI G'GyrC_C

Cuts at:      0     457     639 
   Size:        457     182
 
BanII G_rGCy'C

Cuts at:      0     147     154     162     377     639 
   Size:        147       7       8     215     262

  Fragments arranged by size: 

                 262     215     147       8       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     619     639 
   Size:        619      20
 
BglI GCCn_nnn'nGGC

Cuts at:      0      23     200     388     639 
   Size:         23     177     188     251

  Fragments arranged by size: 

                 251     188     177      23
 
Bme1580I G_kGCm'C

Cuts at:      0     147     154     639 
   Size:        147       7     485

  Fragments arranged by size: 

                 485     147       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     185     199     639 
   Size:        176       9      14     440

  Fragments arranged by size: 

                 440     176      14       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     535     639 
   Size:        535     104
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     639 
   Size:         49     590
 
BsaHI Gr'CG_yC

Cuts at:      0     544     639 
   Size:        544      95
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     204     385     639 
   Size:        204     181     254

  Fragments arranged by size: 

                 254     204     181
 
BseYI C'CCAG_C

Cuts at:      0      84     305     639 
   Size:         84     221     334

  Fragments arranged by size: 

                 334     221      84
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     604     639 
   Size:        377     227      35

  Fragments arranged by size: 

                 377     227      35
 
Bsp1286I G_dGCh'C

Cuts at:      0     147     154     162     377     604     639 
   Size:        147       7       8     215     227      35

  Fragments arranged by size: 

                 227     215     147      35       8       7
 
BspHI T'CATG_A

Cuts at:      0     407     639 
   Size:        407     232
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     194     639 
   Size:        194     445
 
BsrFI r'CCGG_y

Cuts at:      0     176     194     639 
   Size:        176      18     445

  Fragments arranged by size: 

                 445     176      18
 
BstEII G'GTnAC_C

Cuts at:      0      99     438     639 
   Size:         99     339     201

  Fragments arranged by size: 

                 339     201      99
 
Bsu36I CC'TnA_GG

Cuts at:      0      58     187     639 
   Size:         58     129     452

  Fragments arranged by size: 

                 452     129      58
 
BtgI C'CryG_G

Cuts at:      0      95     105     605     639 
   Size:         95      10     500      34

  Fragments arranged by size: 

                 500      95      34      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     116     639 
   Size:        116     523
 
BtsI GCAGTG_nn'

Cuts at:      0     559     639 
   Size:        559      80
 
EaeI y'GGCC_r

Cuts at:      0      92     521     639 
   Size:         92     429     118

  Fragments arranged by size: 

                 429     118      92
 
EcoICRI GAG'CTC

Cuts at:      0     375     639 
   Size:        375     264
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     185     199     639 
   Size:        176       9      14     440

  Fragments arranged by size: 

                 440     176      14       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24      33      49     143     144     151     302     560 
   Size:         24       9      16      94       1       7     151     258

Cuts at:    560     639 
   Size:         79

  Fragments arranged by size: 

                 258     151      94      79      24      16       9       7
                   1
 
HaeII r_GCGC'y

Cuts at:      0      69     639 
   Size:         69     570
 
KpnI G_GTAC'C

Cuts at:      0     461     639 
   Size:        461     178
 
MslI CAynn'nnrTG

Cuts at:      0     399     639 
   Size:        399     240
 
MspA1I CmG'CkG

Cuts at:      0     127     299     578     639 
   Size:        127     172     279      61

  Fragments arranged by size: 

                 279     172     127      61
 
NcoI C'CATG_G

Cuts at:      0     105     639 
   Size:        105     534
 
NspI r_CATG'y

Cuts at:      0     289     639 
   Size:        289     350
 
PasI CC'CwG_GG

Cuts at:      0     147     471     639 
   Size:        147     324     168

  Fragments arranged by size: 

                 324     168     147
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     245     477     639 
   Size:        245     232     162

  Fragments arranged by size: 

                 245     232     162
 
PfoI T'CCnGG_A

Cuts at:      0     226     423     639 
   Size:        226     197     216

  Fragments arranged by size: 

                 226     216     197
 
PpuMI rG'GwC_Cy

Cuts at:      0     302     560     639 
   Size:        302     258      79

  Fragments arranged by size: 

                 302     258      79
 
PspOMI G'GGCC_C

Cuts at:      0     143     150     639 
   Size:        143       7     489

  Fragments arranged by size: 

                 489     143       7
 
PstI C_TGCA'G

Cuts at:      0     630     639 
   Size:        630       9
 
PvuII CAG'CTG

Cuts at:      0     127     578     639 
   Size:        127     451      61

  Fragments arranged by size: 

                 451     127      61
 
SacI G_AGCT'C

Cuts at:      0     377     639 
   Size:        377     262
 
SanDI GG'GwC_CC

Cuts at:      0     302     639 
   Size:        302     337
 
SfcI C'TryA_G

Cuts at:      0     573     626     639 
   Size:        573      53      13

  Fragments arranged by size: 

                 573      53      13
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      23     639 
   Size:         23     616
 
SmaI CCC'GGG

Cuts at:      0     253     639 
   Size:        253     386
 
SmlI C'TyrA_G

Cuts at:      0      28     639 
   Size:         28     611
 
StyI C'CwwG_G

Cuts at:      0     105     329     639 
   Size:        105     224     310

  Fragments arranged by size: 

                 310     224     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     333     484     639 
   Size:        333     151     155

  Fragments arranged by size: 

                 333     155     151
 
XmaI C'CCGG_G

Cuts at:      0     251     639 
   Size:        251     388
 

 Enzymes that do cut:

   Acc65I     AfeI     AleI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI     BglI Bme1580I     BpmI   Bpu10I    BpuEI    BsaHI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrFI   BstEII
   Bsu36I     BtgI    BtgZI     BtsI     EaeI  EcoICRI  Eco57MI EcoO109I
    HaeII     KpnI     MslI   MspA1I     NcoI     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SfcI
     SfiI     SmaI     SmlI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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