(Linear) (Six-base) MAPSORT of: KIBB8031.seq Check: 1850 from: 1 to: 1758 KIBB8031.orf With 182 enzymes: SgfI * May 21, 2013 16:22 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 956 1590 1758 Size: 956 634 168 Fragments arranged by size: 956 634 168 AflIII A'CryG_T Cuts at: 0 950 1758 Size: 950 808 AlwNI CAG_nnn'CTG Cuts at: 0 384 729 777 1758 Size: 384 345 48 981 Fragments arranged by size: 981 384 345 48 ApaI G_GGCC'C Cuts at: 0 405 1758 Size: 405 1353 ApaLI G'TGCA_C Cuts at: 0 374 1758 Size: 374 1384 AvaI C'yCGr_G Cuts at: 0 5 326 354 421 793 1046 1307 1758 Size: 5 321 28 67 372 253 261 451 Fragments arranged by size: 451 372 321 261 253 67 28 5 BanI G'GyrC_C Cuts at: 0 336 417 816 865 1318 1413 1758 Size: 336 81 399 49 453 95 345 Fragments arranged by size: 453 399 345 336 95 81 49 BanII G_rGCy'C Cuts at: 0 196 405 431 1283 1362 1481 1601 1758 Size: 196 209 26 852 79 119 120 157 Fragments arranged by size: 852 209 196 157 120 119 79 26 BbsI GAAGACnn'nnnn_ Cuts at: 0 693 1758 Size: 693 1065 BbvCI CC'TCA_GC Cuts at: 0 538 1758 Size: 538 1220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 219 253 1758 Size: 219 34 1505 Fragments arranged by size: 1505 219 34 BclI T'GATC_A Cuts at: 0 1427 1758 Size: 1427 331 BglI GCCn_nnn'nGGC Cuts at: 0 461 1758 Size: 461 1297 BlpI GC'TnA_GC Cuts at: 0 1117 1758 Size: 1117 641 Bme1580I G_kGCm'C Cuts at: 0 378 397 405 422 821 1418 1758 Size: 378 19 8 17 399 597 340 Fragments arranged by size: 597 399 378 340 19 17 8 BmgBI CAC'GTC Cuts at: 0 1163 1758 Size: 1163 595 BmrI ACTGGGnnnn_n' Cuts at: 0 399 400 1375 1758 Size: 399 1 975 383 Fragments arranged by size: 975 399 383 1 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 196 1758 Size: 196 1562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 691 832 1018 1758 Size: 166 525 141 186 740 Fragments arranged by size: 740 525 186 166 141 Bpu10I CC'TnA_GC Cuts at: 0 538 1758 Size: 538 1220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 961 1758 Size: 961 797 BsaI GGTCTCn'nnnn_ Cuts at: 0 667 1057 1758 Size: 667 390 701 Fragments arranged by size: 701 667 390 BsaHI Gr'CG_yC Cuts at: 0 583 1758 Size: 583 1175 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1226 1256 1334 1364 1758 Size: 1226 30 78 30 394 Fragments arranged by size: 1226 394 78 30 30 BseYI C'CCAG_C Cuts at: 0 39 717 1005 1056 1073 1179 1758 Size: 39 678 288 51 17 106 579 Fragments arranged by size: 678 579 288 106 51 39 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 213 535 638 1639 1758 Size: 213 322 103 1001 119 Fragments arranged by size: 1001 322 213 119 103 BsiHKAI G_wGCw'C Cuts at: 0 378 431 1283 1362 1481 1758 Size: 378 53 852 79 119 277 Fragments arranged by size: 852 378 277 119 79 53 BsmI GAATG_Cn' Cuts at: 0 1044 1758 Size: 1044 714 BsmBI CGTCTCn'nnnn_ Cuts at: 0 325 964 1725 1758 Size: 325 639 761 33 Fragments arranged by size: 761 639 325 33 Bsp1286I G_dGCh'C Cuts at: 0 196 378 397 405 422 431 821 1283 Size: 196 182 19 8 17 9 390 462 Cuts at: 1283 1362 1418 1481 1601 1758 Size: 79 56 63 120 157 Fragments arranged by size: 462 390 196 182 157 120 79 63 56 19 17 9 8 BspHI T'CATG_A Cuts at: 0 1508 1758 Size: 1508 250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1061 1758 Size: 1061 697 BsrBI CCG'CTC Cuts at: 0 1543 1758 Size: 1543 215 BsrDI GCAATG_nn' Cuts at: 0 740 1547 1758 Size: 740 807 211 Fragments arranged by size: 807 740 211 BsrFI r'CCGG_y Cuts at: 0 339 1758 Size: 339 1419 BstAPI GCAn_nnn'nTGC Cuts at: 0 865 1758 Size: 865 893 BstXI CCAn_nnnn'nTGG Cuts at: 0 647 669 1639 1758 Size: 647 22 970 119 Fragments arranged by size: 970 647 119 22 Bsu36I CC'TnA_GG Cuts at: 0 625 632 1729 1758 Size: 625 7 1097 29 Fragments arranged by size: 1097 625 29 7 BtgI C'CryG_G Cuts at: 0 20 200 432 459 646 677 1149 1199 Size: 20 180 232 27 187 31 472 50 Cuts at: 1199 1252 1409 1638 1758 Size: 53 157 229 120 Fragments arranged by size: 472 232 229 187 180 157 120 53 50 31 27 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 198 483 1252 1314 1758 Size: 198 285 769 62 444 Fragments arranged by size: 769 444 285 198 62 ClaI AT'CG_AT Cuts at: 0 1094 1758 Size: 1094 664 DrdI GACnn_nn'nnGTC Cuts at: 0 151 1161 1758 Size: 151 1010 597 Fragments arranged by size: 1010 597 151 EaeI y'GGCC_r Cuts at: 0 341 462 659 680 779 926 1098 1133 Size: 341 121 197 21 99 147 172 35 Cuts at: 1133 1544 1641 1758 Size: 411 97 117 Fragments arranged by size: 411 341 197 172 147 121 117 99 97 35 21 EarI CTCTTCn'nnn_ Cuts at: 0 607 1729 1758 Size: 607 1122 29 Fragments arranged by size: 1122 607 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 281 1072 1758 Size: 281 791 686 Fragments arranged by size: 791 686 281 EcoICRI GAG'CTC Cuts at: 0 429 1281 1360 1479 1758 Size: 429 852 79 119 279 Fragments arranged by size: 852 429 279 119 79 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 691 832 956 1018 1590 1758 Size: 166 525 141 124 62 572 168 Fragments arranged by size: 572 525 168 166 141 124 62 EcoO109I rG'GnC_Cy Cuts at: 0 99 401 636 774 1011 1050 1661 1703 Size: 99 302 235 138 237 39 611 42 Cuts at: 1703 1758 Size: 55 Fragments arranged by size: 611 302 237 235 138 99 55 42 39 HaeII r_GCGC'y Cuts at: 0 58 1199 1758 Size: 58 1141 559 Fragments arranged by size: 1141 559 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 161 193 260 292 1758 Size: 161 32 67 32 1466 Fragments arranged by size: 1466 161 67 32 32 HincII GTy'rAC Cuts at: 0 243 587 1572 1758 Size: 243 344 985 186 Fragments arranged by size: 985 344 243 186 MscI TGG'CCA Cuts at: 0 661 682 781 928 1643 1758 Size: 661 21 99 147 715 115 Fragments arranged by size: 715 661 147 115 99 21 MslI CAynn'nnrTG Cuts at: 0 645 1758 Size: 645 1113 MspA1I CmG'CkG Cuts at: 0 483 762 1758 Size: 483 279 996 Fragments arranged by size: 996 483 279 NaeI GCC'GGC Cuts at: 0 341 1758 Size: 341 1417 NcoI C'CATG_G Cuts at: 0 459 646 1409 1758 Size: 459 187 763 349 Fragments arranged by size: 763 459 349 187 NgoMIV G'CCGG_C Cuts at: 0 339 1758 Size: 339 1419 NspI r_CATG'y Cuts at: 0 523 1570 1758 Size: 523 1047 188 Fragments arranged by size: 1047 523 188 PasI CC'CwG_GG Cuts at: 0 473 822 1440 1758 Size: 473 349 618 318 Fragments arranged by size: 618 473 349 318 PflMI CCAn_nnn'nTGG Cuts at: 0 729 1758 Size: 729 1029 PfoI T'CCnGG_A Cuts at: 0 1283 1362 1758 Size: 1283 79 396 Fragments arranged by size: 1283 396 79 PpuMI rG'GwC_Cy Cuts at: 0 636 774 1011 1050 1758 Size: 636 138 237 39 708 Fragments arranged by size: 708 636 237 138 39 PspOMI G'GGCC_C Cuts at: 0 401 1758 Size: 401 1357 PstI C_TGCA'G Cuts at: 0 773 861 1758 Size: 773 88 897 Fragments arranged by size: 897 773 88 PvuII CAG'CTG Cuts at: 0 483 762 1758 Size: 483 279 996 Fragments arranged by size: 996 483 279 SacI G_AGCT'C Cuts at: 0 431 1283 1362 1481 1758 Size: 431 852 79 119 277 Fragments arranged by size: 852 431 277 119 79 SanDI GG'GwC_CC Cuts at: 0 1011 1758 Size: 1011 747 SapI GCTCTTCn'nnn_ Cuts at: 0 607 1758 Size: 607 1151 SbfI CC_TGCA'GG Cuts at: 0 861 1758 Size: 861 897 SfcI C'TryA_G Cuts at: 0 769 857 1125 1758 Size: 769 88 268 633 Fragments arranged by size: 769 633 268 88 SmaI CCC'GGG Cuts at: 0 7 328 423 1048 1309 1758 Size: 7 321 95 625 261 449 Fragments arranged by size: 625 449 321 261 95 7 SmlI C'TyrA_G Cuts at: 0 976 1758 Size: 976 782 StuI AGG'CCT Cuts at: 0 118 630 933 1141 1758 Size: 118 512 303 208 617 Fragments arranged by size: 617 512 303 208 118 StyI C'CwwG_G Cuts at: 0 149 459 646 746 986 1409 1758 Size: 149 310 187 100 240 423 349 Fragments arranged by size: 423 349 310 240 187 149 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1342 1758 Size: 1342 416 TatI w'GTAC_w Cuts at: 0 839 1758 Size: 839 919 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1226 1258 1334 1366 1758 Size: 1226 32 76 32 392 Fragments arranged by size: 1226 392 76 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 103 219 457 690 1093 1758 Size: 103 116 238 233 403 665 Fragments arranged by size: 665 403 238 233 116 103 XmaI C'CCGG_G Cuts at: 0 5 326 421 1046 1307 1758 Size: 5 321 95 625 261 451 Fragments arranged by size: 625 451 321 261 95 5 Enzymes that do cut: AcuI AflIII AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtgZI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglII BmtI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BspEI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtsI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI