Restriction map for pF1KB5317
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Cfr10I      ClaI        CpoI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        Van91I      XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AflIII        677  ACATGT               A^C[GA][CT]GT
Aor51HI       938  AGCGCT               AGC^GCT
AspHI        1162  GTGCTC               G[AT]GC[AT]^C
AspI          601  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          112  CTCGAG               C^[CT]CG[GA]G
BamHI         366  GGATCC               G^GATCC
BbvCI         669  GCTGAGG              CCTCAGC(-5/-2)
BcgI           46  CGACCCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          250  CTAG                 C^TAG
BlpI          888  GCTGAGC              GC^T[ACGT]AGC
BmyI         1162  GTGCTC               G[AGT]GC[ACT]^C
Bpu1102I      888  GCTGAGC              GC^T[ACGT]AGC
BsaI          292  GAGACC               GGTCTC(1/5)
BsaWI         460  TCCGGT               [AT]^CCGG[AT]
BsgI          751  GTGCAG               GTGCAG(16/14)
BsiHKAI      1162  GTGCTC               G[AT]GC[AT]^C
BsmAI         292  GAGAC                GTCTC(1/5)
BsmFI         770  GGGAC                GGGAC(10/14)
BsoBI         112  CTCGAG               C^[CT]CG[GA]G
Bsp1286I     1162  GTGCTC               G[AGT]GC[ACT]^C
BspLU11I      677  ACATGT               A^CATGT
BspMI        1076  ACCTGC               ACCTGC(4/8)
BstAPI        615  GCAGGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         987  CCAGACCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         888  GCTGAGC              GC^T[ACGT]AGC
CfoI          939  GCGC                 GCG^C
DraI         1155  TTTAAA               TTT^AAA
DsaI          173  CCATGG               C^C[GA][CT]GG
Eco47III      938  AGCGCT               AGC^GCT
EcoNI         609  CCTCCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       173  CCATGG               C^C[AT][AT]GG
FauI          764  CCCGC                CCCGC(4/6)
HaeII         938  AGCGCT               [GA]GCGC^[CT]
HhaI          939  GCGC                 GCG^C
HinP1I        939  GCGC                 G^CGC
HphI           15  GGTGA                GGTGA(8/7)
MaeI          250  CTAG                 C^TAG
MseI         1156  TTAA                 T^TAA
MspA1I        757  CAGCTG               C[AC]G^C[GT]G
NcoI          173  CCATGG               C^CATGG
NspI          677  ACATGT               [GA]CATG^[CT]
PaeR7I        112  CTCGAG               C^TCGAG
PflFI         601  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         757  CAGCTG               CAG^CTG
SbfI          612  CCTGCAGG             CCTGCA^GG
SexAI         710  ACCAGGT              A^CC[AT]GGT
Sse8387I      612  CCTGCAGG             CCTGCA^GG
StyI          173  CCATGG               C^C[AT][AT]GG
TfiI          457  GATTC                G^A[AT]TC
Tru9I        1156  TTAA                 T^TAA
Tsp45I        731  GTGAC                ^GT[GC]AC
Tth111I       601  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           81  CCAGTGTTTCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          112  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI          141  GAATTT               [GA]^AATT[CT]
              193  GAATTT              
AocI          541  CCTGAGG              CC^T[ACGT]AGG
             1001  CCTCAGG             
ApoI          141  GAATTT               [GA]^AATT[CT]
              193  GAATTT              
BanI          721  GGTGCC               G^G[CT][GA]CC
             1128  GGTGCC              
BbsI          355  GAAGAC               GAAGAC(2/6)
             1072  GAAGAC              
BcnI           66  CCCGG                CC^[GC]GG
              768  CCGGG               
BpmI          360  CTGGAG               CTGGAG(16/14)
              778  CTGGAG              
BpuAI         355  GAAGAC               GAAGAC(2/6)
             1072  GAAGAC              
BsaJI         173  CCATGG               C^C[ACGT][ACGT]GG
              593  CCCAGG              
BstNI         594  CCAGG                CC^[AT]GG
              711  CCAGG               
Bsu36I        541  CCTGAGG              CC^T[ACGT]AGG
             1001  CCTCAGG             
Eco81I        541  CCTGAGG              CC^T[ACGT]AGG
             1001  CCTCAGG             
EcoRII        594  CCAGG                ^CC[AT]GG
              711  CCAGG               
HaeIII          6  GGCC                 GG^CC
              762  GGCC                
MaeIII        270  GTTAC                ^GT[ACGT]AC
              731  GTGAC               
MvaI          594  CCAGG                CC^[AT]GG
              711  CCAGG               
NciI           66  CCCGG                CC^[GC]GG
              768  CCGGG               
PleI          601  GACTC                GAGTC(4/5)
               18  GAGTC               


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BbvI        BseRI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
Fnu4HI      FokI        HapII       HinfI       HpaII       
ItaI        Ksp632I     MboI        MboII       MflI        
MnlI        MspI        MwoI        NdeII       NlaIII      
NlaIV       PpuMI       PstI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com