################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstYI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MseI MspA1I MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 525 TCCGGA T^CCGGA AluI 269 AGCT AG^CT Alw44I 130 GTGCAC G^TGCAC AlwNI 182 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 187 CCTGAGG CC^T[ACGT]AGG ApaI 241 GGGCCC GGGCC^C ApaLI 130 GTGCAC G^TGCAC AvaI 951 CCCGAG C^[CT]CG[GA]G BalI 1022 TGGCCA TGG^CCA BanI 565 GGTGCC G^G[CT][GA]CC BbsI 55 GTCTTC GAAGAC(2/6) BpmI 106 CTGGAG CTGGAG(16/14) BpuAI 55 GTCTTC GAAGAC(2/6) BsaAI 667 TACGTG [CT]AC^GT[GA] BsaI 12 GAGACC GGTCTC(1/5) BsaWI 525 TCCGGA [AT]^CCGG[AT] BseAI 525 TCCGGA T^CCGGA BseRI 27 GAGGAG GAGGAG(10/8) BsgI 290 CTGCAC GTGCAG(16/14) BsiEI 543 CGACCG CG[GA][CT]^CG BsmAI 12 GAGAC GTCTC(1/5) BsmI 968 GCATTC GAATGC(1/-1) BsoBI 951 CCCGAG C^[CT]CG[GA]G BspEI 525 TCCGGA T^CCGGA BssHII 601 GCGCGC G^CGCGC Bsu36I 187 CCTGAGG CC^T[ACGT]AGG DdeI 188 CTGAG C^T[ACGT]AG DrdI 544 GACCGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 187 CCTGAGG CC^T[ACGT]AGG EcoT14I 926 CCATGG C^C[AT][AT]GG MluNI 1022 TGGCCA TGG^CCA MroI 525 TCCGGA T^CCGGA MscI 1022 TGGCCA TGG^CCA NaeI 869 GCCGGC GCC^GGC NcoI 926 CCATGG C^CATGG NgoMIV 869 GCCGGC G^CCGGC NlaIII 927 CATG CATG^ PflMI 874 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 959 CTGCAG CTGCA^G SfcI 959 CTGCAG C^T[GA][CT]AG StyI 926 CCATGG C^C[AT][AT]GG TfiI 143 GATTC G^A[AT]TC Van91I 874 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 220 GTCTAC GT^[AC][GT]AC 394 GTCTAC AspHI 130 GTGCAC G[AT]GC[AT]^C 324 GAGCAC BanII 241 GGGCCC G[GA]GC[CT]^C 693 GGGCTC BcnI 239 CCGGG CC^[GC]GG 738 CCCGG BsiHKAI 130 GTGCAC G[AT]GC[AT]^C 324 GAGCAC BspMI 844 ACCTGC ACCTGC(4/8) 441 GCAGGT BssSI 589 CTCGTG CACGAG(-5/-1) 676 CTCGTG BstXI 584 CCAGCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 830 CCACCTTTTTGG DsaI 926 CCATGG C^C[GA][CT]GG 1019 CCGTGG EarI 409 CTCTTC CTCTTC(1/4) 24 GAAGAG Ksp632I 409 CTCTTC CTCTTC(1/4) 24 GAAGAG MaeII 668 ACGT A^CGT 794 ACGT MslI 327 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 657 CACAGCGATG NciI 239 CCGGG CC^[GC]GG 738 CCCGG PleI 672 GACTC GAGTC(4/5) 64 GAGTC PpuMI 537 AGGTCCC [GA]G^G[AT]CC[CT] 905 AGGACCT SfaNI 625 GATGC GCATC(5/9) 18 GCATC TaqI 941 TCGA T^CGA 972 TCGA TthHB8I 941 TCGA T^CGA 972 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AfaI AlwI AvaII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII EaeI EcoO109I EcoO65I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI