Restriction map for pF1KB4623
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsiEI       BsiWI       BsmBI       BsoBI       
BspDI       BspHI       BspLU11I    BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
MfeI        MluI        MseI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tru9I       
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
AccIII        851  TCCGGA               T^CCGGA
AflIII       1176  ACACGT               A^C[GA][CT]GT
BalI          983  TGGCCA               TGG^CCA
BamHI         431  GGATCC               G^GATCC
BanI          166  GGTGCC               G^G[CT][GA]CC
BanII        1462  GAGCTC               G[GA]GC[CT]^C
BbsI          769  GAAGAC               GAAGAC(2/6)
BcnI           26  CCGGG                CC^[GC]GG
BfaI         1195  CTAG                 C^TAG
BglI          634  GCCTGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1083  AGATCT               A^GATCT
BpuAI         769  GAAGAC               GAAGAC(2/6)
BsaBI        1078  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          333  GGTCTC               GGTCTC(1/5)
BsaWI         851  TCCGGA               [AT]^CCGG[AT]
BseAI         851  TCCGGA               T^CCGGA
BsgI          437  GTGCAG               GTGCAG(16/14)
BsmAI         334  GTCTC                GTCTC(1/5)
BsmFI         484  GGGAC                GGGAC(10/14)
BsmI         1326  GCATTC               GAATGC(1/-1)
BspEI         851  TCCGGA               T^CCGGA
BspMI        1206  GCAGGT               ACCTGC(4/8)
BsrBI         926  CCGCTC               CCGCTC(-3/-3)
BstUI          52  CGCG                 CG^CG
CfoI           41  GCGC                 GCG^C
CpoI          644  CGGACCG              CG^G[AT]CCG
DdeI          785  CTCAG                C^T[ACGT]AG
DraII         484  GGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          983  TGGCCA               [CT]^GGCC[GA]
EcoNI         804  CCTTATAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      484  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         203  GAATTC               G^AATTC
EcoT14I      1319  CCATGG               C^C[AT][AT]GG
HhaI           41  GCGC                 GCG^C
HinP1I         41  GCGC                 G^CGC
KspI           51  CCGCGG               CCGC^GG
MaeI         1195  CTAG                 C^TAG
MamI         1078  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         983  TGGCCA               TGG^CCA
MroI          851  TCCGGA               T^CCGGA
MscI          983  TGGCCA               TGG^CCA
MslI         1536  CATGCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         51  CCGCGG               C[AC]G^C[GT]G
MvnI           52  CGCG                 CG^CG
NciI           26  CCGGG                CC^[GC]GG
NcoI         1319  CCATGG               C^CATGG
NdeI          341  CATATG               CA^TATG
NspI          947  GCATGT               [GA]CATG^[CT]
PflMI        1375  CCATTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1452  GACTC                GAGTC(4/5)
PpuMI         484  GGGACCC              [GA]G^G[AT]CC[CT]
RsrII         644  CGGACCG              CG^G[AT]CCG
SacI         1462  GAGCTC               GAGCT^C
SacII          51  CCGCGG               CCGC^GG
SexAI          67  ACCTGGT              A^CC[AT]GGT
SmlI          931  CTTGAG               C^T[CT][GA]AG
StuI         1473  AGGCCT               AGG^CCT
StyI         1319  CCATGG               C^C[AT][AT]GG
TaqI         1181  TCGA                 T^CGA
TthHB8I      1181  TCGA                 T^CGA
Van91I       1375  CCATTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI         104  GTGCTC               G[AT]GC[AT]^C
             1462  GAGCTC              
BbvI         1545  GCAGC                GCAGC(8/12)
              709  GCTGC               
BseRI          16  CTCCTC               GAGGAG(10/8)
               21  CTCCTC              
BsiHKAI       104  GTGCTC               G[AT]GC[AT]^C
             1462  GAGCTC              
Bsp1407I      795  TGTACA               T^GTACA
             1366  TGTACA              
BsrGI         795  TGTACA               T^GTACA
             1366  TGTACA              
BstEII       1222  GGTGACC              G^GT[ACGT]ACC
             1314  GGTCACC             
BstPI        1222  GGTGACC              G^GT[ACGT]ACC
             1314  GGTCACC             
BstXI         170  CCACGGTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1304  CCAAAGTGGTGG        
EarI          336  CTCTTC               CTCTTC(1/4)
             1497  GAAGAG              
EcoO65I      1222  GGTGACC              G^GT[ACGT]ACC
             1314  GGTCACC             
FauI           50  CCCGC                CCCGC(4/6)
              443  GCGGG               
HapII          26  CCGG                 C^CGG
              852  CCGG                
HpaII          26  CCGG                 C^CGG
              852  CCGG                
Ksp632I       336  CTCTTC               CTCTTC(1/4)
             1497  GAAGAG              
MspI           26  CCGG                 C^CGG
              852  CCGG                
PstI          257  CTGCAG               CTGCA^G
              710  CTGCAG              
ScaI          374  AGTACT               AGT^ACT
             1501  AGTACT              
SfcI          257  CTGCAG               C^T[GA][CT]AG
              710  CTGCAG              
SspBI         795  TGTACA               T^GTACA
             1366  TGTACA              
TseI          709  GCTGC                G^C[AT]GC
             1545  GCAGC               
Tsp45I       1223  GTGAC                ^GT[GC]AC
             1315  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BmyI        BpmI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DpnII       DsaI        EcoRII      Fnu4HI      
FokI        HaeIII      HincII      HindII      HinfI       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        Tsp509I     
TspRI       XhoII       
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