Restriction map for pF1KB4623
 (Linear) (Six-base) MAPSORT of: KIBB4623.seq  Check: 8558  from: 1  to: 1560



KIBB4623.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AflIII A'CryG_T

Cuts at:      0    1176    1560 
   Size:       1176     384
 
ApoI r'AATT_y

Cuts at:      0     203     361     684    1560 
   Size:        203     158     323     876

  Fragments arranged by size: 

                 876     323     203     158
 
BamHI G'GATC_C

Cuts at:      0     431    1560 
   Size:        431    1129
 
BanI G'GyrC_C

Cuts at:      0     166    1560 
   Size:        166    1394
 
BanII G_rGCy'C

Cuts at:      0    1466    1560 
   Size:       1466      94
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     776    1560 
   Size:        776     784
 
BglI GCCn_nnn'nGGC

Cuts at:      0     640    1560 
   Size:        640     920
 
BglII A'GATC_T

Cuts at:      0    1083    1560 
   Size:       1083     477
 
Bme1580I G_kGCm'C

Cuts at:      0     418    1560 
   Size:        418    1142
 
BmgBI CAC'GTC

Cuts at:      0    1179    1560 
   Size:       1179     381
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1514    1560 
   Size:       1514      46
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     944    1560 
   Size:        944     616
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     470     749     911    1125    1560 
   Size:        260     210     279     162     214     435

  Fragments arranged by size: 

                 435     279     260     214     210     162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     952    1560 
   Size:        952     608
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     339    1560 
   Size:        339    1221
 
BsaBI GATnn'nnATC

Cuts at:      0    1082    1560 
   Size:       1082     478
 
BsaWI w'CCGG_w

Cuts at:      0     851    1560 
   Size:        851     709
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     884     914    1560 
   Size:        884      30     646

  Fragments arranged by size: 

                 884     646      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7      12    1560 
   Size:          7       5    1548

  Fragments arranged by size: 

                1548       7       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458    1560 
   Size:        458    1102
 
BsiHKAI G_wGCw'C

Cuts at:      0     108    1466    1560 
   Size:        108    1358      94

  Fragments arranged by size: 

                1358     108      94
 
BsmI GAATG_Cn'

Cuts at:      0    1326    1560 
   Size:       1326     234
 
Bsp1286I G_dGCh'C

Cuts at:      0     108     418    1466    1560 
   Size:        108     310    1048      94

  Fragments arranged by size: 

                1048     310     108      94
 
BspEI T'CCGG_A

Cuts at:      0     851    1560 
   Size:        851     709
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1197    1560 
   Size:       1197     363
 
BsrBI CCG'CTC

Cuts at:      0     928    1560 
   Size:        928     632
 
BsrDI GCAATG_nn'

Cuts at:      0     542    1166    1396    1560 
   Size:        542     624     230     164

  Fragments arranged by size: 

                 624     542     230     164
 
BsrGI T'GTAC_A

Cuts at:      0     795    1366    1560 
   Size:        795     571     194

  Fragments arranged by size: 

                 795     571     194
 
BstEII G'GTnAC_C

Cuts at:      0    1222    1314    1560 
   Size:       1222      92     246

  Fragments arranged by size: 

                1222     246      92
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177    1311    1560 
   Size:        177    1134     249

  Fragments arranged by size: 

                1134     249     177
 
BstYI r'GATC_y

Cuts at:      0      55     132     431    1083    1560 
   Size:         55      77     299     652     477

  Fragments arranged by size: 

                 652     477     299      77      55
 
BtgI C'CryG_G

Cuts at:      0      51     170    1319    1560 
   Size:         51     119    1149     241

  Fragments arranged by size: 

                1149     241     119      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      94     784    1160    1216    1560 
   Size:         94     690     376      56     344

  Fragments arranged by size: 

                 690     376     344      94      56
 
BtsI GCAGTG_nn'

Cuts at:      0     540    1560 
   Size:        540    1020
 
EaeI y'GGCC_r

Cuts at:      0     983    1560 
   Size:        983     577
 
EarI CTCTTCn'nnn_

Cuts at:      0     342    1492    1560 
   Size:        342    1150      68

  Fragments arranged by size: 

                1150     342      68
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     658    1560 
   Size:        658     902
 
EcoICRI GAG'CTC

Cuts at:      0    1464    1560 
   Size:       1464      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     470     749     911    1125    1560 
   Size:        260     210     279     162     214     435

  Fragments arranged by size: 

                 435     279     260     214     210     162
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     808    1560 
   Size:        808     752
 
EcoO109I rG'GnC_Cy

Cuts at:      0     485    1560 
   Size:        485    1075
 
EcoRI G'AATT_C

Cuts at:      0     203    1560 
   Size:        203    1357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     497     529     829     861    1273    1305    1560 
   Size:        497      32     300      32     412      32     255

  Fragments arranged by size: 

                 497     412     300     255      32      32      32
 
HincII GTy'rAC

Cuts at:      0     282    1398    1451    1560 
   Size:        282    1116      53     109

  Fragments arranged by size: 

                1116     282     109      53
 
MscI TGG'CCA

Cuts at:      0     985    1560 
   Size:        985     575
 
MslI CAynn'nnrTG

Cuts at:      0    1540    1560 
   Size:       1540      20
 
MspA1I CmG'CkG

Cuts at:      0      53    1560 
   Size:         53    1507
 
NcoI C'CATG_G

Cuts at:      0    1319    1560 
   Size:       1319     241
 
NdeI CA'TA_TG

Cuts at:      0     342    1560 
   Size:        342    1218
 
NspI r_CATG'y

Cuts at:      0     951    1560 
   Size:        951     609
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1381    1560 
   Size:       1381     179
 
PfoI T'CCnGG_A

Cuts at:      0    1122    1560 
   Size:       1122     438
 
PpuMI rG'GwC_Cy

Cuts at:      0     485    1560 
   Size:        485    1075
 
PsiI TTA'TAA

Cuts at:      0     808    1560 
   Size:        808     752
 
PstI C_TGCA'G

Cuts at:      0     261     714    1560 
   Size:        261     453     846

  Fragments arranged by size: 

                 846     453     261
 
RsrII CG'GwC_CG

Cuts at:      0     645    1560 
   Size:        645     915
 
SacI G_AGCT'C

Cuts at:      0    1466    1560 
   Size:       1466      94
 
SacII CC_GC'GG

Cuts at:      0      54    1560 
   Size:         54    1506
 
SanDI GG'GwC_CC

Cuts at:      0     485    1560 
   Size:        485    1075
 
ScaI AGT'ACT

Cuts at:      0     376    1503    1560 
   Size:        376    1127      57

  Fragments arranged by size: 

                1127     376      57
 
SexAI A'CCwGG_T

Cuts at:      0      67    1560 
   Size:         67    1493
 
SfcI C'TryA_G

Cuts at:      0     257     710    1560 
   Size:        257     453     850

  Fragments arranged by size: 

                 850     453     257
 
SmlI C'TyrA_G

Cuts at:      0     931    1560 
   Size:        931     629
 
StuI AGG'CCT

Cuts at:      0    1475    1560 
   Size:       1475      85
 
StyI C'CwwG_G

Cuts at:      0    1319    1560 
   Size:       1319     241
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     299     662    1496    1560 
   Size:        299     363     834      64

  Fragments arranged by size: 

                 834     363     299      64
 
TatI w'GTAC_w

Cuts at:      0     374     795    1366    1501    1560 
   Size:        374     421     571     135      59

  Fragments arranged by size: 

                 571     421     374     135      59
 

 Enzymes that do cut:

   AflIII     ApoI    BamHI     BanI    BanII     BbsI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaBI
    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrGI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I   HincII     MscI     MslI   MspA1I     NcoI
     NdeI     NspI    PflMI     PfoI    PpuMI     PsiI     PstI    RsrII
     SacI    SacII    SanDI     ScaI    SexAI     SfcI     SmlI     StuI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BlpI     BmtI   Bpu10I    BsaAI
    BsaHI    BseYI    BsiEI    BsiWI    BsmBI    BspHI    BsrFI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII     SalI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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