Restriction map for pF1KB6604
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsiEI       BsiWI       BsmFI       BsmI        BspDI       
BspLU11I    BspMI       BsrDI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DdeI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        MaeI        MamI        
MfeI        MluI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PinAI       
PleI        PmeI        PpuMI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tru9I       XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Alw44I        109  GTGCAC               G^TGCAC
AlwNI        1138  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         109  GTGCAC               G^TGCAC
AspI          391  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          114  CTCGAG               C^[CT]CG[GA]G
BbrPI           4  CACGTG               CAC^GTG
BbsI          328  GTCTTC               GAAGAC(2/6)
BglI          946  GCCAACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         328  GTCTTC               GAAGAC(2/6)
BsaAI           4  CACGTG               [CT]AC^GT[GA]
BsaI         1019  GAGACC               GGTCTC(1/5)
BsgI         1135  GTGCAG               GTGCAG(16/14)
BsoBI         114  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      257  TGTACA               T^GTACA
BspHI         398  TCATGA               T^CATGA
BsrFI         128  ACCGGC               [GA]^CCGG[CT]
BsrGI         257  TGTACA               T^GTACA
BstXI         266  CCATGGCGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        128  ACCGGC               [GA]^CCGG[CT]
DraIII        700  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          622  CTCTTC               CTCTTC(1/4)
EcoT14I       266  CCATGG               C^C[AT][AT]GG
HincII        409  GTCAAC               GT[CT]^[GA]AC
HindII        409  GTCAAC               GT[CT]^[GA]AC
HinfI         613  GAATC                G^A[ACGT]TC
HphI          380  TCACC                GGTGA(8/7)
Ksp632I       622  CTCTTC               CTCTTC(1/4)
KspI          715  CCGCGG               CCGC^GG
MaeIII        505  GTCAC                ^GT[ACGT]AC
MspA1I        715  CCGCGG               C[AC]G^C[GT]G
NcoI          266  CCATGG               C^CATGG
PaeR7I        114  CTCGAG               C^TCGAG
PflFI         391  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         699  CCACTGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI           4  CACGTG               CAC^GTG
PmlI            4  CACGTG               CAC^GTG
PshAI        1140  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          733  CTGCAG               CTGCA^G
RcaI          398  TCATGA               T^CATGA
SacII         715  CCGCGG               CCGC^GG
SfcI          733  CTGCAG               C^T[GA][CT]AG
SgrAI         127  CACCGGCG             C[GA]^CCGG[CT]G
SmlI          114  CTCGAG               C^T[CT][GA]AG
SspBI         257  TGTACA               T^GTACA
StuI          983  AGGCCT               AGG^CCT
StyI          266  CCATGG               C^C[AT][AT]GG
TfiI          613  GAATC                G^A[AT]TC
Tsp45I        505  GTCAC                ^GT[GC]AC
Tth111I       391  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        699  CCACTGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          239  CCATGTTCATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          114  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccIII        521  TCCGGA               T^CCGGA
              569  TCCGGA              
AcsI          433  GAATTC               [GA]^AATT[CT]
              517  GAATTC              
ApoI          433  GAATTC               [GA]^AATT[CT]
              517  GAATTC              
BalI          353  TGGCCA               TGG^CCA
              944  TGGCCA              
BbeI           27  GGCGCC               GGCGC^C
              131  GGCGCC              
BpmI          214  CTCCAG               CTGGAG(16/14)
              592  CTCCAG              
BseAI         521  TCCGGA               T^CCGGA
              569  TCCGGA              
BseRI         277  GAGGAG               GAGGAG(10/8)
              658  GAGGAG              
BsmBI         562  GAGACG               CGTCTC(1/5)
              748  GAGACG              
BspEI         521  TCCGGA               T^CCGGA
              569  TCCGGA              
BsrBI         906  CCGCTC               CCGCTC(-3/-3)
              148  GAGCGG              
BsrI          701  ACTGG                ACTGG(1/-1)
             1037  ACTGG               
BssSI         997  CACGAG               CACGAG(-5/-1)
              415  CTCGTG              
EcoRI         433  GAATTC               G^AATTC
              517  GAATTC              
KasI           27  GGCGCC               G^GCGCC
              131  GGCGCC              
MluNI         353  TGGCCA               TGG^CCA
              944  TGGCCA              
MroI          521  TCCGGA               T^CCGGA
              569  TCCGGA              
MscI          353  TGGCCA               TGG^CCA
              944  TGGCCA              
NarI           27  GGCGCC               GG^CGCC
              131  GGCGCC              
SfoI           27  GGCGCC               GGC^GCC
              131  GGCGCC              
Tsp509I       434  AATT                 ^AATT
              518  AATT                
TspRI          14  CACTG                CA[GC]TG[ACGT][ACGT]^
              700  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BsaHI       
BsaJI       BsaWI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       ItaI        MaeII       MboI        
MboII       MflI        MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TseI        TthHB8I     
XhoII       
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