Restriction map for pF1KB6604
 (Linear) (Six-base) MAPSORT of: KIBB6604.seq  Check: 2715  from: 1  to: 1293



KIBB6604.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     315    1073    1270    1293 
   Size:         75     240     758     197      23

  Fragments arranged by size: 

                 758     240     197      75      23
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1220    1293 
   Size:       1220      73
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1143    1293 
   Size:       1143     150
 
ApaI G_GGCC'C

Cuts at:      0     293     346     809    1293 
   Size:        293      53     463     484

  Fragments arranged by size: 

                 484     463     293      53
 
ApaLI G'TGCA_C

Cuts at:      0     109    1293 
   Size:        109    1184
 
ApoI r'AATT_y

Cuts at:      0     433     517    1293 
   Size:        433      84     776

  Fragments arranged by size: 

                 776     433      84
 
AvaI C'yCGr_G

Cuts at:      0     114    1293 
   Size:        114    1179
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     624     657    1293 
   Size:        624      33     636

  Fragments arranged by size: 

                 636     624      33
 
BanI G'GyrC_C

Cuts at:      0      27      54     131     234    1170    1293 
   Size:         27      27      77     103     936     123

  Fragments arranged by size: 

                 936     123     103      77      27      27
 
BanII G_rGCy'C

Cuts at:      0     293     346     809    1293 
   Size:        293      53     463     484

  Fragments arranged by size: 

                 484     463     293      53
 
BbeI G_GCGC'C

Cuts at:      0      31     135    1293 
   Size:         31     104    1158

  Fragments arranged by size: 

                1158     104      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     321    1293 
   Size:        321     972
 
BglI GCCn_nnn'nGGC

Cuts at:      0     952    1293 
   Size:        952     341
 
Bme1580I G_kGCm'C

Cuts at:      0      57     113     293     346     809    1175    1293 
   Size:         57      56     180      53     463     366     118

  Fragments arranged by size: 

                 463     366     180     118      57      56      53
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     711    1293 
   Size:        711     582
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     129     192    1293 
   Size:        129      63    1101

  Fragments arranged by size: 

                1101     129      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     577    1293 
   Size:        199     378     716

  Fragments arranged by size: 

                 716     378     199
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1013    1293 
   Size:       1013     280
 
BsaAI yAC'GTr

Cuts at:      0       6    1293 
   Size:          6    1287
 
BsaHI Gr'CG_yC

Cuts at:      0      28     132     386     916    1293 
   Size:         28     104     254     530     377

  Fragments arranged by size: 

                 530     377     254     104      28
 
BsaWI w'CCGG_w

Cuts at:      0     467     521     569     872    1293 
   Size:        467      54      48     303     421

  Fragments arranged by size: 

                 467     421     303      54      48
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     292     673    1293 
   Size:        292     381     620

  Fragments arranged by size: 

                 620     381     292
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1156    1293 
   Size:       1156     137
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     593     602    1293 
   Size:        113     480       9     691

  Fragments arranged by size: 

                 691     480     113       9
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     556     742    1293 
   Size:        556     186     551

  Fragments arranged by size: 

                 556     551     186
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     113     293     346     593     602     809    1175 
   Size:         57      56     180      53     247       9     207     366

Cuts at:   1175    1293 
   Size:        118

  Fragments arranged by size: 

                 366     247     207     180     118      57      56      53
                   9
 
BspEI T'CCGG_A

Cuts at:      0     521     569    1293 
   Size:        521      48     724

  Fragments arranged by size: 

                 724     521      48
 
BspHI T'CATG_A

Cuts at:      0     398    1293 
   Size:        398     895
 
BsrBI CCG'CTC

Cuts at:      0     150     908    1293 
   Size:        150     758     385

  Fragments arranged by size: 

                 758     385     150
 
BsrFI r'CCGG_y

Cuts at:      0     128    1293 
   Size:        128    1165
 
BsrGI T'GTAC_A

Cuts at:      0     257    1293 
   Size:        257    1036
 
BssSI C'ACGA_G

Cuts at:      0     415     997    1293 
   Size:        415     582     296

  Fragments arranged by size: 

                 582     415     296
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     273    1293 
   Size:        273    1020
 
BstYI r'GATC_y

Cuts at:      0     170     485     524     618    1293 
   Size:        170     315      39      94     675

  Fragments arranged by size: 

                 675     315     170      94      39
 
BtgI C'CryG_G

Cuts at:      0     266     715     848     878    1293 
   Size:        266     449     133      30     415

  Fragments arranged by size: 

                 449     415     266     133      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1029    1293 
   Size:       1029     264
 
BtsI GCAGTG_nn'

Cuts at:      0      13    1293 
   Size:         13    1280
 
DraIII CAC_nnn'GTG

Cuts at:      0     705    1293 
   Size:        705     588
 
EaeI y'GGCC_r

Cuts at:      0      83     297     353     944    1293 
   Size:         83     214      56     591     349

  Fragments arranged by size: 

                 591     349     214      83      56
 
EarI CTCTTCn'nnn_

Cuts at:      0     628    1293 
   Size:        628     665
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      66    1293 
   Size:         66    1227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     199     315     577    1073    1270    1293 
   Size:         75     124     116     262     496     197      23

  Fragments arranged by size: 

                 496     262     197     124     116      75      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0     342     343     806    1293 
   Size:        342       1     463     487

  Fragments arranged by size: 

                 487     463     342       1
 
EcoRI G'AATT_C

Cuts at:      0     433     517    1293 
   Size:        433      84     776

  Fragments arranged by size: 

                 776     433      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     345     453     840    1293 
   Size:        345     108     387     453

  Fragments arranged by size: 

                 453     387     345     108
 
HaeII r_GCGC'y

Cuts at:      0      31      49     135    1293 
   Size:         31      18      86    1158

  Fragments arranged by size: 

                1158      86      31      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1219    1251    1293 
   Size:       1219      32      42

  Fragments arranged by size: 

                1219      42      32
 
HincII GTy'rAC

Cuts at:      0     411    1293 
   Size:        411     882
 
KasI G'GCGC_C

Cuts at:      0      27     131    1293 
   Size:         27     104    1162

  Fragments arranged by size: 

                1162     104      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1262    1293 
   Size:       1262      31
 
MscI TGG'CCA

Cuts at:      0     355     946    1293 
   Size:        355     591     347

  Fragments arranged by size: 

                 591     355     347
 
MslI CAynn'nnrTG

Cuts at:      0     244     271     397    1293 
   Size:        244      27     126     896

  Fragments arranged by size: 

                 896     244     126      27
 
MspA1I CmG'CkG

Cuts at:      0     717    1293 
   Size:        717     576
 
NarI GG'CG_CC

Cuts at:      0      28     132    1293 
   Size:         28     104    1161

  Fragments arranged by size: 

                1161     104      28
 
NcoI C'CATG_G

Cuts at:      0     266    1293 
   Size:        266    1027
 
PasI CC'CwG_GG

Cuts at:      0     339    1293 
   Size:        339     954
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     705    1293 
   Size:        705     588
 
PfoI T'CCnGG_A

Cuts at:      0     542     593    1293 
   Size:        542      51     700

  Fragments arranged by size: 

                 700     542      51
 
PmlI CAC'GTG

Cuts at:      0       6    1293 
   Size:          6    1287
 
PshAI GACnn'nnGTC

Cuts at:      0    1144    1293 
   Size:       1144     149
 
PspOMI G'GGCC_C

Cuts at:      0     289     342     805    1293 
   Size:        289      53     463     488

  Fragments arranged by size: 

                 488     463     289      53
 
PspXI vC'TCGA_Gb

Cuts at:      0     114    1293 
   Size:        114    1179
 
PstI C_TGCA'G

Cuts at:      0     737    1293 
   Size:        737     556
 
SacII CC_GC'GG

Cuts at:      0     718    1293 
   Size:        718     575
 
SfcI C'TryA_G

Cuts at:      0     733    1293 
   Size:        733     560
 
SfoI GGC'GCC

Cuts at:      0      29     133    1293 
   Size:         29     104    1160

  Fragments arranged by size: 

                1160     104      29
 
SgrAI Cr'CCGG_yG

Cuts at:      0     128    1293 
   Size:        128    1165
 
SmlI C'TyrA_G

Cuts at:      0     114    1293 
   Size:        114    1179
 
StuI AGG'CCT

Cuts at:      0     985    1293 
   Size:        985     308
 
StyI C'CwwG_G

Cuts at:      0     266    1293 
   Size:        266    1027
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     252     458     693    1293 
   Size:        252     206     235     600

  Fragments arranged by size: 

                 600     252     235     206
 
TatI w'GTAC_w

Cuts at:      0     257    1293 
   Size:        257    1036
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      47      79    1293 
   Size:         47      32    1214

  Fragments arranged by size: 

                1214      47      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     394    1293 
   Size:        394     899
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     246    1293 
   Size:        246    1047
 
XhoI C'TCGA_G

Cuts at:      0     114    1293 
   Size:        114    1179
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI
     BanI    BanII     BbeI     BbsI     BglI Bme1580I     BmrI     BplI
     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseRI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI    BssSI
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     PasI
    PflMI     PfoI     PmlI    PshAI   PspOMI    PspXI     PstI    SacII
     SfcI     SfoI    SgrAI     SmlI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     AscI     AseI    AsiSI    AvrII
    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI   Bpu10I    BpuEI    BsaBI    BsaXI    BseYI    BsiEI
    BsiWI     BsmI    BspMI    BsrDI   BssHII   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI
    EcoNI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PpiI    PpuMI     PsiI
     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     XbaI     XmaI     XmnI     ZraI
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