Restriction map for pF1KE1041
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrI        BssHII      BssSI       Bst1107I    BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MroI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        StyI        
SwaI        TseI        Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AciI          775  GCGG                 CCGC(-3/-1)
AcsI          760  GAATTC               [GA]^AATT[CT]
AhdI          745  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         350  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          309  GGGCCC               GGGCC^C
ApoI          760  GAATTC               [GA]^AATT[CT]
AspEI         745  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         526  GGTCC                G^G[AT]CC
BalI           31  TGGCCA               TGG^CCA
BanII         309  GGGCCC               G[GA]GC[CT]^C
BbsI          869  GTCTTC               GAAGAC(2/6)
BfaI          590  CTAG                 C^TAG
BpuAI         869  GTCTTC               GAAGAC(2/6)
BsaAI         726  CACGTA               [CT]AC^GT[GA]
BsmAI         795  GTCTC                GTCTC(1/5)
BsmFI         536  GTCCC                GGGAC(10/14)
BsmI          502  GAATGC               GAATGC(1/-1)
Bsp1407I      549  TGTACA               T^GTACA
BspMI         180  ACCTGC               ACCTGC(4/8)
BsrGI         549  TGTACA               T^GTACA
BstAPI        398  GCACAGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         584  TTCGAA               TT^CGAA
BstEII       1039  GGTTACC              G^GT[ACGT]ACC
BstPI        1039  GGTTACC              G^GT[ACGT]ACC
DpnII         739  GATC                 ^GATC
DraII         308  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EaeI           31  TGGCCA               [CT]^GGCC[GA]
Eam1105I      745  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          916  GAAGAG               CTCTTC(1/4)
EcoO109I      308  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      1039  GGTTACC              G^GT[ACGT]ACC
EcoRI         760  GAATTC               G^AATTC
Fnu4HI        775  GCGGC                GC^[ACGT]GC
ItaI          775  GCGGC                GC^[ACGT]GC
Ksp632I       916  GAAGAG               CTCTTC(1/4)
MaeI          590  CTAG                 C^TAG
MboI          739  GATC                 ^GATC
MluNI          31  TGGCCA               TGG^CCA
MscI           31  TGGCCA               TGG^CCA
MslI          811  CACAGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        813  CAGCTG               C[AC]G^C[GT]G
NdeII         739  GATC                 ^GATC
NspV          584  TTCGAA               TT^CGAA
PflMI         349  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          751  GAGTC                GAGTC(4/5)
PstI          229  CTGCAG               CTGCA^G
PvuII         813  CAGCTG               CAG^CTG
SapI          916  GAAGAGC              GCTCTTC(1/4)
Sau3AI        739  GATC                 ^GATC
SexAI         483  ACCTGGT              A^CC[AT]GGT
SfcI          229  CTGCAG               C^T[GA][CT]AG
SfuI          584  TTCGAA               TT^CGAA
SmlI          510  CTCAAG               C^T[CT][GA]AG
SspBI         549  TGTACA               T^GTACA
SspI            5  AATATT               AAT^ATT
Van91I        349  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BmyI           75  GTGCCC               G[AGT]GC[ACT]^C
              309  GGGCCC              
BsiYI         349  CCAGCTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              989  CCAAAAAGAGG         
BslI          349  CCAGCTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              989  CCAAAAAGAGG         
Bsp1286I       75  GTGCCC               G[AGT]GC[ACT]^C
              309  GGGCCC              
BssKI         355  CCTGG                ^CC[ACGT]GG
              484  CCTGG               
BstF5I         28  GGATG                GGATG(2/0)
              771  GGATG               
BstNI         355  CCTGG                CC^[AT]GG
              484  CCTGG               
EcoRII        355  CCTGG                ^CC[AT]GG
              484  CCTGG               
FokI           28  GGATG                GGATG(9/13)
              771  GGATG               
HaeIII         32  GGCC                 GG^CC
              310  GGCC                
MaeII         600  ACGT                 A^CGT
              727  ACGT                
MvaI          355  CCTGG                CC^[AT]GG
              484  CCTGG               
MwoI          231  GCAGAGATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              398  GCACAGATTGC         
NlaIII        395  CATG                 CATG^
              651  CATG                
NlaIV          54  GGAACC               GG[ACGT]^[ACGT]CC
              309  GGGCCC              
ScrFI         355  CCTGG                CC^[ACGT]GG
              484  CCTGG               
SfaNI         772  GATGC                GCATC(5/9)
              578  GCATC               
Tsp45I        818  GTGAC                ^GT[GC]AC
             1027  GTGAC               
TspRI         520  CAGTG                CA[GC]TG[ACGT][ACGT]^
              767  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AfaI        AluI        BpmI        BsrDI       Cfr13I      
DdeI        HinfI       MaeIII      MboII       MnlI        
MseI        RsaI        Sau96I      TaqI        TfiI        
Tru9I       Tsp509I     TthHB8I     
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