Restriction map for pF1KB3452
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanII       BbeI        BbsI        BbvCI       
BcgI        BciVI       BfaI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoT22I     
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MslI        MspA1I      MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII          19  CGCG                 CG^CG
AfaI           30  GTAC                 GT^AC
AflII         163  CTTAAG               C^TTAAG
AlwI          124  GATCC                GGATC(4/5)
Aor51HI       140  AGCGCT               AGC^GCT
AspHI         621  GAGCAC               G[AT]GC[AT]^C
AvaI          147  CTCGGG               C^[CT]CG[GA]G
AvaII         207  GGACC                G^G[AT]CC
BanI          151  GGCACC               G^G[CT][GA]CC
BbrPI         511  CACGTG               CAC^GTG
BclI          347  TGATCA               T^GATCA
BcnI          603  CCGGG                CC^[GC]GG
BfrI          163  CTTAAG               C^TTAAG
BpmI           85  CTGGAG               CTGGAG(16/14)
BsaAI         511  CACGTG               [CT]AC^GT[GA]
BsgI           52  CTGCAC               GTGCAG(16/14)
BsiHKAI       621  GAGCAC               G[AT]GC[AT]^C
BsmBI          88  GAGACG               CGTCTC(1/5)
BsmFI         206  GGGAC                GGGAC(10/14)
BsoBI         147  CTCGGG               C^[CT]CG[GA]G
BsrDI         575  CATTGC               GCAATG(2/0)
BstF5I        444  GGATG                GGATG(2/0)
BstUI          19  CGCG                 CG^CG
DraI          381  TTTAAA               TTT^AAA
Eco47III      140  AGCGCT               AGC^GCT
EcoNI          27  CCTGTACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         241  GATATC               GAT^ATC
EcoT14I       626  CCATGG               C^C[AT][AT]GG
FbaI          347  TGATCA               T^GATCA
FokI          444  GGATG                GGATG(9/13)
HapII         603  CCGG                 C^CGG
HincII        559  GTCAAC               GT[CT]^[GA]AC
HindII        559  GTCAAC               GT[CT]^[GA]AC
HpaII         603  CCGG                 C^CGG
MaeII         512  ACGT                 A^CGT
MspI          603  CCGG                 C^CGG
MvnI           19  CGCG                 CG^CG
NciI          603  CCGGG                CC^[GC]GG
NcoI          626  CCATGG               C^CATGG
NruI           18  TCGCGA               TCG^CGA
PleI          340  GAGTC                GAGTC(4/5)
PmaCI         511  CACGTG               CAC^GTG
PmlI          511  CACGTG               CAC^GTG
RsaI           30  GTAC                 GT^AC
SfaNI         322  GATGC                GCATC(5/9)
SfcI          497  CTGTAG               C^T[GA][CT]AG
StyI          626  CCATGG               C^C[AT][AT]GG
TaqI          260  TCGA                 T^CGA
TspRI         217  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       260  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BalI          308  TGGCCA               TGG^CCA
              434  TGGCCA              
BbvI            5  GCAGC                GCAGC(8/12)
              213  GCAGC               
BmyI          150  GGGCAC               G[AGT]GC[ACT]^C
              621  GAGCAC              
Bsp1286I      150  GGGCAC               G[AGT]GC[ACT]^C
              621  GAGCAC              
BsrI          294  ACTGG                ACTGG(1/-1)
              590  ACTGG               
DsaI          236  CCGTGG               C^C[GA][CT]GG
              626  CCATGG              
EaeI          308  TGGCCA               [CT]^GGCC[GA]
              434  TGGCCA              
FauI          176  CCCGC                CCCGC(4/6)
               42  GCGGG               
HphI          450  GGTGA                GGTGA(8/7)
              549  TCACC               
MaeIII        221  GTGAC                ^GT[ACGT]AC
              509  GTCAC               
MluNI         308  TGGCCA               TGG^CCA
              434  TGGCCA              
MscI          308  TGGCCA               TGG^CCA
              434  TGGCCA              
MseI          164  TTAA                 T^TAA
              382  TTAA                
SmlI          163  CTTAAG               C^T[CT][GA]AG
              580  CTCAAG              
TfiI          357  GATTC                G^A[AT]TC
              365  GAATC               
Tru9I         164  TTAA                 T^TAA
              382  TTAA                
TseI            5  GCAGC                G^C[AT]GC
              213  GCAGC               
Tsp45I        221  GTGAC                ^GT[GC]AC
              509  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AluI        BsaJI       BsiYI       BslI        
BsmAI       BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
HaeII       HaeIII      HhaI        HinP1I      HinfI       
ItaI        MboI        MboII       MnlI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       
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