Restriction map for pF1KB7921
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AfaI        AflII       
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        KspI        MaeI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tru9I       Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcyI          111  GACGCC               G[GA]^CG[CT]C
AflIII        454  ACGTGT               A^C[GA][CT]GT
AlwNI         191  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         400  GAGCTC               G[AT]GC[AT]^C
AvaI          214  CTCGGG               C^[CT]CG[GA]G
BbrPI         453  CACGTG               CAC^GTG
BbsI          327  GAAGAC               GAAGAC(2/6)
BbvCI         132  GCTGAGG              CCTCAGC(-5/-2)
BcnI          465  CCGGG                CC^[GC]GG
BpmI          485  CTCCAG               CTGGAG(16/14)
BpuAI         327  GAAGAC               GAAGAC(2/6)
BsaAI         453  CACGTG               [CT]AC^GT[GA]
BsaHI         111  GACGCC               G[GA]^CG[CT]C
BsaI           24  GGTCTC               GGTCTC(1/5)
BsaWI         167  ACCGGA               [AT]^CCGG[AT]
BseRI         397  GAGGAG               GAGGAG(10/8)
BsiHKAI       400  GAGCTC               G[AT]GC[AT]^C
BsmAI          25  GTCTC                GTCTC(1/5)
BsmI          372  GAATGC               GAATGC(1/-1)
BsoBI         214  CTCGGG               C^[CT]CG[GA]G
BsrDI         533  CATTGC               GCAATG(2/0)
BsrI           84  ACTGG                ACTGG(1/-1)
Cac8I         338  GCCTGC               GC[ACGT]^[ACGT]GC
CfoI          181  GCGC                 GCG^C
Cfr13I        449  GGCCC                G^G[ACGT]CC
EarI          127  GAAGAG               CTCTTC(1/4)
HgaI          111  GACGC                GACGC(5/10)
HhaI          181  GCGC                 GCG^C
Hin1I         111  GACGCC               G[GA]^CG[CT]C
HinP1I        181  GCGC                 G^CGC
Ksp632I       127  GAAGAG               CTCTTC(1/4)
MaeII         454  ACGT                 A^CGT
MspA1I        296  CAGCTG               C[AC]G^C[GT]G
MwoI          235  GCCGAGGTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          465  CCGGG                CC^[GC]GG
NlaIII        432  CATG                 CATG^
NspI          431  ACATGC               [GA]CATG^[CT]
PmaCI         453  CACGTG               CAC^GTG
PmlI          453  CACGTG               CAC^GTG
PstI          340  CTGCAG               CTGCA^G
PvuII         296  CAGCTG               CAG^CTG
SacI          400  GAGCTC               GAGCT^C
SapI          127  GAAGAGC              GCTCTTC(1/4)
Sau96I        449  GGCCC                G^G[ACGT]CC
SfaNI           3  GATGC                GCATC(5/9)
SfcI          340  CTGCAG               C^T[GA][CT]AG
SmlI          403  CTCAAG               C^T[CT][GA]AG
TfiI          187  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BanII         400  GAGCTC               G[GA]GC[CT]^C
              467  GGGCTC              
BmyI          400  GAGCTC               G[AGT]GC[ACT]^C
              467  GGGCTC              
BsiYI          15  CCCAGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               71  CCCCTCCTGGG         
BslI           15  CCCAGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               71  CCCCTCCTGGG         
BsmFI          58  GTCCC                GGGAC(10/14)
               69  GTCCC               
Bsp1286I      400  GAGCTC               G[AGT]GC[ACT]^C
              467  GGGCTC              
BstF5I        144  CATCC                GGATG(2/0)
              170  GGATG               
FokI          144  CATCC                GGATG(9/13)
              170  GGATG               
HaeIII         19  GGCC                 GG^CC
              449  GGCC                
HapII         168  CCGG                 C^CGG
              465  CCGG                
HpaII         168  CCGG                 C^CGG
              465  CCGG                
MaeIII         81  GTCAC                ^GT[ACGT]AC
              193  GTCAC               
MspI          168  CCGG                 C^CGG
              465  CCGG                
PleI          483  GACTC                GAGTC(4/5)
              349  GAGTC               
Tsp45I         81  GTCAC                ^GT[GC]AC
              193  GTCAC               
TspRI          83  CACTG                CA[GC]TG[ACGT][ACGT]^
              195  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BbvI        BsaJI       BssKI       BstNI       
DdeI        EcoRII      Fnu4HI      HinfI       ItaI        
MboII       MnlI        MvaI        ScrFI       TseI        
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