Restriction map for pF1KB5769
##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AspI        BbeI        BbrPI       BciVI       
BclI        BfrI        BsaAI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstZ17I     ClaI        
CpoI        DraI        DraIII      Eco47III    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        KasI        
MluI        MroI        NarI        NdeI        NotI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SspBI       SspI        StuI        
SwaI        Tth111I     XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

Acc65I       2057  GGTACC               G^GTACC
AcsI          699  AAATTC               [GA]^AATT[CT]
AcyI          194  GACGCC               G[GA]^CG[CT]C
AhdI          505  GACTTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          699  AAATTC               [GA]^AATT[CT]
AseI         2016  ATTAAT               AT^TAAT
AsnI         2016  ATTAAT               AT^TAAT
Asp700I       459  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2057  GGTACC               G^GTACC
AspEI         505  GACTTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1494  GAGCTC               G[AT]GC[AT]^C
AvaI           65  CTCGGG               C^[CT]CG[GA]G
AviII        1535  TGCGCA               TGC^GCA
AvrII          39  CCTAGG               C^CTAGG
BbsI          502  GAAGAC               GAAGAC(2/6)
BcgI          921  GCAGGCCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          916  GCCTTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         832  AGATCT               A^GATCT
BlnI           39  CCTAGG               C^CTAGG
BlpI         1419  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1419  GCTGAGC              GC^T[ACGT]AGC
BpuAI         502  GAAGAC               GAAGAC(2/6)
BsaBI        2098  GATAAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         194  GACGCC               G[GA]^CG[CT]C
BsaWI        1891  ACCGGA               [AT]^CCGG[AT]
BsgI         1207  CTGCAC               GTGCAG(16/14)
BsiHKAI      1494  GAGCTC               G[AT]GC[AT]^C
BsmBI         751  GAGACG               CGTCTC(1/5)
BsoBI          65  CTCGGG               C^[CT]CG[GA]G
BsrDI        1686  CATTGC               GCAATG(2/0)
BssHII       1283  GCGCGC               G^CGCGC
BstAPI       2182  GCAGCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         161  TTCGAA               TT^CGAA
CelII        1419  GCTGAGC              GC^T[ACGT]AGC
DrdI         1835  GACAAGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      505  GACTTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         492  CCTCTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1535  TGCGCA               TGC^GCA
Hin1I         194  GACGCC               G[GA]^CG[CT]C
HincII       1053  GTTAAC               GT[CT]^[GA]AC
HindII       1053  GTTAAC               GT[CT]^[GA]AC
HpaI         1053  GTTAAC               GTT^AAC
KpnI         2057  GGTACC               GGTAC^C
KspI         1412  CCGCGG               CCGC^GG
MaeII        1202  ACGT                 A^CGT
MamI         2098  GATAAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          298  CAATTG               C^AATTG
MunI          298  CAATTG               C^AATTG
NaeI         1130  GCCGGC               GCC^GGC
NcoI          767  CCATGG               C^CATGG
NgoMIV       1130  GCCGGC               G^CCGGC
NheI          379  GCTAGC               G^CTAGC
NruI         1559  TCGCGA               TCG^CGA
NspI          625  GCATGC               [GA]CATG^[CT]
NspV          161  TTCGAA               TT^CGAA
PpuMI         844  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2016  ATTAAT               AT^TAAT
SacI         1494  GAGCTC               GAGCT^C
SacII        1412  CCGCGG               CCGC^GG
SbfI         1584  CCTGCAGG             CCTGCA^GG
ScaI         1861  AGTACT               AGT^ACT
SfuI          161  TTCGAA               TT^CGAA
SphI          625  GCATGC               GCATG^C
Sse8387I     1584  CCTGCAGG             CCTGCA^GG
XbaI         1710  TCTAGA               T^CTAGA
XmnI          459  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AocI          814  CCTCAGG              CC^T[ACGT]AGG
             1106  CCTCAGG             
BamHI          35  GGATCC               G^GATCC
               70  GGATCC              
BbvCI         882  CCTCAGC              CCTCAGC(-5/-2)
             1479  CCTCAGC             
BsaI         1566  GAGACC               GGTCTC(1/5)
             1607  GAGACC              
BspMI        1151  ACCTGC               ACCTGC(4/8)
              123  GCAGGT              
BssSI        1231  CACGAG               CACGAG(-5/-1)
             1255  CACGAG              
BstEII       1794  GGTCACC              G^GT[ACGT]ACC
             1887  GGTCACC             
BstPI        1794  GGTCACC              G^GT[ACGT]ACC
             1887  GGTCACC             
BstXI          22  CCAAGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1356  CCAAGGGAGTGG        
Bsu36I        814  CCTCAGG              CC^T[ACGT]AGG
             1106  CCTCAGG             
DsaI          767  CCATGG               C^C[GA][CT]GG
             1412  CCGCGG              
EagI         1176  CGGCCG               C^GGCCG
             1347  CGGCCG              
EclXI        1176  CGGCCG               C^GGCCG
             1347  CGGCCG              
Eco52I       1176  CGGCCG               C^GGCCG
             1347  CGGCCG              
Eco81I        814  CCTCAGG              CC^T[ACGT]AGG
             1106  CCTCAGG             
EcoO65I      1794  GGTCACC              G^GT[ACGT]ACC
             1887  GGTCACC             
HaeII        1098  AGCGCC               [GA]GCGC^[CT]
             1423  AGCGCC              
HindIII      1762  AAGCTT               A^AGCTT
             1903  AAGCTT              
MseI         1054  TTAA                 T^TAA
             2017  TTAA                
MslI         1878  CATTCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1957  CATTTCCGTG          
PvuII        2206  CAGCTG               CAG^CTG
             2215  CAGCTG              
SexAI         578  ACCTGGT              A^CC[AT]GGT
             1610  ACCAGGT             
SfaNI         649  GATGC                GCATC(5/9)
             2220  GATGC               
Tru9I        1054  TTAA                 T^TAA
             2017  TTAA                
Tsp45I       1795  GTCAC                ^GT[GC]AC
             1888  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiEI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        TaqI        TfiI        
TseI        Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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