Restriction map for pF1KB5769
 (Linear) (Six-base) MAPSORT of: KIBB5769.seq  Check: 5093  from: 1  to: 2250



KIBB5769.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     114    2250 
   Size:        114    2136
 
Acc65I G'GTAC_C

Cuts at:      0    2057    2250 
   Size:       2057     193
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     478     808     813    1921    2250 
   Size:        158     320     330       5    1108     329

  Fragments arranged by size: 

                1108     330     329     320     158       5
 
AhdI GACnn_n'nnGTC

Cuts at:      0     510    2250 
   Size:        510    1740
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1222    2250 
   Size:       1222    1028
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     748     780    2250 
   Size:        748      32    1470

  Fragments arranged by size: 

                1470     748      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     622     645     666     786     948    1932    2250 
   Size:        622      23      21     120     162     984     318

  Fragments arranged by size: 

                 984     622     318     162     120      23      21
 
ApaI G_GGCC'C

Cuts at:      0      52    1039    1511    2250 
   Size:         52     987     472     739

  Fragments arranged by size: 

                 987     739     472      52
 
ApoI r'AATT_y

Cuts at:      0     699    2250 
   Size:        699    1551
 
AseI AT'TA_AT

Cuts at:      0    2017    2250 
   Size:       2017     233
 
AvaI C'yCGr_G

Cuts at:      0      65    2250 
   Size:         65    2185
 
AvrII C'CTAG_G

Cuts at:      0      39    2250 
   Size:         39    2211
 
BamHI G'GATC_C

Cuts at:      0      35      70    2250 
   Size:         35      35    2180

  Fragments arranged by size: 

                2180      35      35
 
BanI G'GyrC_C

Cuts at:      0    1277    1516    1662    1935    2057    2250 
   Size:       1277     239     146     273     122     193

  Fragments arranged by size: 

                1277     273     239     193     146     122
 
BanII G_rGCy'C

Cuts at:      0      52     375     448     563    1039    1405    1498    1511 
   Size:         52     323      73     115     476     366      93      13

Cuts at:   1511    1899    2250 
   Size:        388     351

  Fragments arranged by size: 

                 476     388     366     351     323     115      93      73
                  52      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     509    2250 
   Size:        509    1741
 
BbvCI CC'TCA_GC

Cuts at:      0     883    1480    2250 
   Size:        883     597     770

  Fragments arranged by size: 

                 883     770     597
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     910     944    2250 
   Size:        910      34    1306

  Fragments arranged by size: 

                1306     910      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     922    2250 
   Size:        922    1328
 
BglII A'GATC_T

Cuts at:      0     832    2250 
   Size:        832    1418
 
BlpI GC'TnA_GC

Cuts at:      0    1420    2250 
   Size:       1420     830
 
Bme1580I G_kGCm'C

Cuts at:      0      52      87    1039    1511    1667    2250 
   Size:         52      35     952     472     156     583

  Fragments arranged by size: 

                 952     583     472     156      52      35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     116    1041    2250 
   Size:        116     925    1209

  Fragments arranged by size: 

                1209     925     116
 
BmtI G_CTAG'C

Cuts at:      0     383    2250 
   Size:        383    1867
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1355    2250 
   Size:       1355     895
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     896    1405    2086    2250 
   Size:        361     535     509     681     164

  Fragments arranged by size: 

                 681     535     509     361     164
 
Bpu10I CC'TnA_GC

Cuts at:      0     374     883    1021    1480    2250 
   Size:        374     509     138     459     770

  Fragments arranged by size: 

                 770     509     459     374     138
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1560    1601    2250 
   Size:       1560      41     649

  Fragments arranged by size: 

                1560     649      41
 
BsaBI GATnn'nnATC

Cuts at:      0    2102    2250 
   Size:       2102     148
 
BsaHI Gr'CG_yC

Cuts at:      0     195    2250 
   Size:        195    2055
 
BsaWI w'CCGG_w

Cuts at:      0    1891    2250 
   Size:       1891     359
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     334     364     748     778    1444    1474    1646    1676 
   Size:        334      30     384      30     666      30     172      30

Cuts at:   1676    2045    2075    2250 
   Size:        369      30     175

  Fragments arranged by size: 

                 666     384     369     334     175     172      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      53     358     386    1095    1459    2250 
   Size:         53     305      28     709     364     791

  Fragments arranged by size: 

                 791     709     364     305      53      28
 
BseYI C'CCAG_C

Cuts at:      0      45      74     234     448     542    1112    1666    1925 
   Size:         45      29     160     214      94     570     554     259

Cuts at:   1925    2178    2250 
   Size:        253      72

  Fragments arranged by size: 

                 570     554     259     253     214     160      94      72
                  45      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1192    2250 
   Size:       1192    1058
 
BsiEI CG_ry'CG

Cuts at:      0    1179    1346    1350    2250 
   Size:       1179     167       4     900

  Fragments arranged by size: 

                1179     900     167       4
 
BsiHKAI G_wGCw'C

Cuts at:      0    1498    2250 
   Size:       1498     752
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     745    2250 
   Size:        745    1505
 
Bsp1286I G_dGCh'C

Cuts at:      0      52      87     375     448     563    1039    1405    1498 
   Size:         52      35     288      73     115     476     366      93

Cuts at:   1498    1511    1667    1899    2250 
   Size:         13     156     232     351

  Fragments arranged by size: 

                 476     366     351     288     232     156     115      93
                  73      52      35      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     114    1160    2250 
   Size:        114    1046    1090

  Fragments arranged by size: 

                1090    1046     114
 
BsrBI CCG'CTC

Cuts at:      0    1305    1593    1739    2250 
   Size:       1305     288     146     511

  Fragments arranged by size: 

                1305     511     288     146
 
BsrDI GCAATG_nn'

Cuts at:      0    1685    2250 
   Size:       1685     565
 
BsrFI r'CCGG_y

Cuts at:      0      11    1130    1345    2250 
   Size:         11    1119     215     905

  Fragments arranged by size: 

                1119     905     215      11
 
BssHII G'CGCG_C

Cuts at:      0    1283    2250 
   Size:       1283     967
 
BssSI C'ACGA_G

Cuts at:      0    1231    1255    2250 
   Size:       1231      24     995

  Fragments arranged by size: 

                1231     995      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2188    2250 
   Size:       2188      62
 
BstBI TT'CG_AA

Cuts at:      0     162    2250 
   Size:        162    2088
 
BstEII G'GTnAC_C

Cuts at:      0    1794    1887    2250 
   Size:       1794      93     363

  Fragments arranged by size: 

                1794     363      93
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      29    1363    2250 
   Size:         29    1334     887

  Fragments arranged by size: 

                1334     887      29
 
BstYI r'GATC_y

Cuts at:      0      35      70     487     832    2250 
   Size:         35      35     417     345    1418

  Fragments arranged by size: 

                1418     417     345      35      35
 
Bsu36I CC'TnA_GG

Cuts at:      0     815    1107    2250 
   Size:        815     292    1143

  Fragments arranged by size: 

                1143     815     292
 
BtgI C'CryG_G

Cuts at:      0     767    1412    2250 
   Size:        767     645     838

  Fragments arranged by size: 

                 838     767     645
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1542    2250 
   Size:       1542     708
 
BtsI GCAGTG_nn'

Cuts at:      0    1757    1942    1999    2250 
   Size:       1757     185      57     251

  Fragments arranged by size: 

                1757     251     185      57
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1841    2250 
   Size:       1841     409
 
EaeI y'GGCC_r

Cuts at:      0      19     117     294    1176    1227    1347    2155    2250 
   Size:         19      98     177     882      51     120     808      95

  Fragments arranged by size: 

                 882     808     177     120      98      95      51      19
            
 
EagI C'GGCC_G

Cuts at:      0    1176    1347    2250 
   Size:       1176     171     903

  Fragments arranged by size: 

                1176     903     171
 
EarI CTCTTCn'nnn_

Cuts at:      0     784    1436    1558    2248    2250 
   Size:        784     652     122     690       2

  Fragments arranged by size: 

                 784     690     652     122       2
 
EcoICRI GAG'CTC

Cuts at:      0    1496    2250 
   Size:       1496     754
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     361     478     808     813     896    1405    1921 
   Size:        158     203     117     330       5      83     509     516

Cuts at:   1921    2086    2250 
   Size:        165     164

  Fragments arranged by size: 

                 516     509     330     203     165     164     158     117
                  83       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     496    2250 
   Size:        496    1754
 
EcoO109I rG'GnC_Cy

Cuts at:      0      49     357     538     619     845     924    1035    1507 
   Size:         49     308     181      81     226      79     111     472

Cuts at:   1507    2250 
   Size:        743

  Fragments arranged by size: 

                 743     472     308     226     181     111      81      79
                  49
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1785    2250 
   Size:       1785     465
 
FspI TGC'GCA

Cuts at:      0    1537    2250 
   Size:       1537     713
 
HaeII r_GCGC'y

Cuts at:      0    1102    1427    2250 
   Size:       1102     325     823

  Fragments arranged by size: 

                1102     823     325
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      62      94     334     366     496     528     661     693 
   Size:         62      32     240      32     130      32     133      32

Cuts at:    693    1353    1385    1444    1476    2250 
   Size:        660      32      59      32     774

  Fragments arranged by size: 

                 774     660     240     133     130      62      59      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1055    2250 
   Size:       1055    1195
 
HindIII A'AGCT_T

Cuts at:      0    1762    1903    2250 
   Size:       1762     141     347

  Fragments arranged by size: 

                1762     347     141
 
HpaI GTT'AAC

Cuts at:      0    1055    2250 
   Size:       1055    1195
 
KpnI G_GTAC'C

Cuts at:      0    2061    2250 
   Size:       2061     189
 
MfeI C'AATT_G

Cuts at:      0     298    2250 
   Size:        298    1952
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     301    1229    1366    1430    1610    2250 
   Size:        200     101     928     137      64     180     640

  Fragments arranged by size: 

                 928     640     200     180     137     101      64
 
MscI TGG'CCA

Cuts at:      0      21     119    1229    2250 
   Size:         21      98    1110    1021

  Fragments arranged by size: 

                1110    1021      98      21
 
MslI CAynn'nnrTG

Cuts at:      0    1882    1961    2250 
   Size:       1882      79     289

  Fragments arranged by size: 

                1882     289      79
 
MspA1I CmG'CkG

Cuts at:      0     596    1123    1414    1470    1647    2208    2217    2250 
   Size:        596     527     291      56     177     561       9      33

  Fragments arranged by size: 

                 596     561     527     291     177      56      33       9
            
 
NaeI GCC'GGC

Cuts at:      0    1132    2250 
   Size:       1132    1118
 
NcoI C'CATG_G

Cuts at:      0     767    2250 
   Size:        767    1483
 
NgoMIV G'CCGG_C

Cuts at:      0    1130    2250 
   Size:       1130    1120
 
NheI G'CTAG_C

Cuts at:      0     379    2250 
   Size:        379    1871
 
NruI TCG'CGA

Cuts at:      0    1561    2250 
   Size:       1561     689
 
NspI r_CATG'y

Cuts at:      0     629    2250 
   Size:        629    1621
 
PasI CC'CwG_GG

Cuts at:      0    2200    2250 
   Size:       2200      50
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      14     966    1932    2124    2250 
   Size:         14     952     966     192     126

  Fragments arranged by size: 

                 966     952     192     126      14
 
PfoI T'CCnGG_A

Cuts at:      0     467     721     947    1913    2063    2250 
   Size:        467     254     226     966     150     187

  Fragments arranged by size: 

                 966     467     254     226     187     150
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     748     780    2250 
   Size:        748      32    1470

  Fragments arranged by size: 

                1470     748      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     845    2250 
   Size:        845    1405
 
PspOMI G'GGCC_C

Cuts at:      0      48    1035    1507    2250 
   Size:         48     987     472     743

  Fragments arranged by size: 

                 987     743     472      48
 
PstI C_TGCA'G

Cuts at:      0     641    1247    1589    2250 
   Size:        641     606     342     661

  Fragments arranged by size: 

                 661     641     606     342
 
PvuII CAG'CTG

Cuts at:      0    2208    2217    2250 
   Size:       2208       9      33

  Fragments arranged by size: 

                2208      33       9
 
SacI G_AGCT'C

Cuts at:      0    1498    2250 
   Size:       1498     752
 
SacII CC_GC'GG

Cuts at:      0    1415    2250 
   Size:       1415     835
 
SanDI GG'GwC_CC

Cuts at:      0     845    2250 
   Size:        845    1405
 
SbfI CC_TGCA'GG

Cuts at:      0    1589    2250 
   Size:       1589     661
 
ScaI AGT'ACT

Cuts at:      0    1863    2250 
   Size:       1863     387
 
SexAI A'CCwGG_T

Cuts at:      0     578    1610    2250 
   Size:        578    1032     640

  Fragments arranged by size: 

                1032     640     578
 
SfcI C'TryA_G

Cuts at:      0     637    1243    1585    2250 
   Size:        637     606     342     665

  Fragments arranged by size: 

                 665     637     606     342
 
SphI G_CATG'C

Cuts at:      0     629    2250 
   Size:        629    1621
 
StyI C'CwwG_G

Cuts at:      0      39     767    1005    1356    2250 
   Size:         39     728     238     351     894

  Fragments arranged by size: 

                 894     728     351     238      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     593    1035    1308    2250 
   Size:        593     442     273     942

  Fragments arranged by size: 

                 942     593     442     273
 
TatI w'GTAC_w

Cuts at:      0     569    1861    2250 
   Size:        569    1292     389

  Fragments arranged by size: 

                1292     569     389
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1646    1678    2250 
   Size:       1646      32     572

  Fragments arranged by size: 

                1646     572      32
 
XbaI T'CTAG_A

Cuts at:      0    1710    2250 
   Size:       1710     540
 
XmnI GAAnn'nnTTC

Cuts at:      0     463    2250 
   Size:        463    1787
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AhdI     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI     NruI
     NspI     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI
    PvuII     SacI    SacII    SanDI     SbfI     ScaI    SexAI     SfcI
     SphI     StyI    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AleI
    ApaLI     AscI    AsiSI     BaeI     BbeI    BciVI     BclI    BfrBI
    BmgBI    BpuEI    BsaAI    BsiWI     BsmI    BspEI    BspHI    BsrGI
  BstZ17I     ClaI    CspCI     DraI   DraIII     EciI    EcoRI    EcoRV
     FseI    FspAI     KasI     MluI     NarI     NdeI     NotI     NsiI
     PacI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SalI     SapI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
  Tth111I     XcmI     XhoI     XmaI     ZraI
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