Restriction map for pF1KB6369
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BciVI       BclI        BcnI        BfrI        BglI        
BlnI        BmyI        BpmI        BsaAI       BsaHI       
BsaI        BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        KasI        KpnI        KspI        
MfeI        MluI        MslI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScrFI       SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccIII         21  TCCGGA               T^CCGGA
AciI          203  CCGC                 CCGC(-3/-1)
BalI          715  TGGCCA               TGG^CCA
BcgI          153  CGATTCAAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         306  AGATCT               A^GATCT
BlpI          163  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      163  GCTCAGC              GC^T[ACGT]AGC
BsaBI          13  GATCTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         536  CCTTGG               C^C[ACGT][ACGT]GG
BsaWI          21  TCCGGA               [AT]^CCGG[AT]
BseAI          21  TCCGGA               T^CCGGA
BspEI          21  TCCGGA               T^CCGGA
CelII         163  GCTCAGC              GC^T[ACGT]AGC
EaeI          715  TGGCCA               [CT]^GGCC[GA]
EarI          283  GAAGAG               CTCTTC(1/4)
EcoT14I       536  CCTTGG               C^C[AT][AT]GG
HaeIII        716  GGCC                 GG^CC
HapII          22  CCGG                 C^CGG
HindIII       794  AAGCTT               A^AGCTT
HpaII          22  CCGG                 C^CGG
HphI          149  TCACC                GGTGA(8/7)
Ksp632I       283  GAAGAG               CTCTTC(1/4)
MaeII         260  ACGT                 A^CGT
MaeIII         49  GTTAC                ^GT[ACGT]AC
MamI           13  GATCTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         715  TGGCCA               TGG^CCA
MroI           21  TCCGGA               T^CCGGA
MscI          715  TGGCCA               TGG^CCA
MspA1I        203  CCGCTG               C[AC]G^C[GT]G
MspI           22  CCGG                 C^CGG
MwoI          732  GCTGCTGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          689  CATATG               CA^TATG
PleI          334  GAGTC                GAGTC(4/5)
ScaI           28  AGTACT               AGT^ACT
SfcI           32  CTACAG               C^T[GA][CT]AG
StyI          536  CCTTGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

Asp700I       238  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              256  GAAGACGTTC          
BfaI           16  CTAG                 C^TAG
              550  CTAG                
BsmAI         300  GAGAC                GTCTC(1/5)
              759  GAGAC               
BstF5I        763  CATCC                GGATG(2/0)
              387  GGATG               
FokI          763  CATCC                GGATG(9/13)
              387  GGATG               
MaeI           16  CTAG                 C^TAG
              550  CTAG                
MnlI          565  CCTC                 CCTC(7/6)
               10  GAGG                
NlaIII        637  CATG                 CATG^
              682  CATG                
SmlI          528  CTTGAG               C^T[CT][GA]AG
              756  CTTGAG              
TspRI         429  CAGTG                CA[GC]TG[ACGT][ACGT]^
              632  CACTG               
XbaI           15  TCTAGA               T^CTAGA
              549  TCTAGA              
XmnI          238  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              256  GAAGACGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AfaI        AluI        AlwI        BbsI        BbvI        
BpuAI       BstYI       DdeI        DpnII       Fnu4HI      
HinfI       ItaI        MboI        MboII       MflI        
MseI        NdeII       RsaI        Sau3AI      TfiI        
Tru9I       TseI        Tsp509I     XhoII       
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