Restriction map for pF1KB0958
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsgI        
BsiEI       BsiWI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeIII      MamI        MfeI        MflI        
MluI        MroI        MspA1I      MunI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AflII        1231  CTTAAG               C^TTAAG
AlwI         1027  GATCC                GGATC(4/5)
AspHI         808  GAGCAC               G[AT]GC[AT]^C
AvaI           28  CCCGAG               C^[CT]CG[GA]G
BalI          410  TGGCCA               TGG^CCA
BanII         954  GAGCCC               G[GA]GC[CT]^C
BfaI         1162  CTAG                 C^TAG
BfrI         1231  CTTAAG               C^TTAAG
BsaWI          50  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       808  GAGCAC               G[AT]GC[AT]^C
BsoBI          28  CCCGAG               C^[CT]CG[GA]G
CfoI          242  GCGC                 GCG^C
DsaI          907  CCACGG               C^C[GA][CT]GG
EaeI          410  TGGCCA               [CT]^GGCC[GA]
EcoT14I       680  CCTTGG               C^C[AT][AT]GG
HaeIII        411  GGCC                 GG^CC
HapII          51  CCGG                 C^CGG
HhaI          242  GCGC                 GCG^C
HinP1I        242  GCGC                 G^CGC
HpaII          51  CCGG                 C^CGG
MaeI         1162  CTAG                 C^TAG
MluNI         410  TGGCCA               TGG^CCA
MscI          410  TGGCCA               TGG^CCA
MspI           51  CCGG                 C^CGG
NdeI          378  CATATG               CA^TATG
PshAI         376  GACATATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          951  GAAGAGC              GCTCTTC(1/4)
SmlI         1231  CTTAAG               C^T[CT][GA]AG
StyI          680  CCTTGG               C^C[AT][AT]GG
TaqI          701  TCGA                 T^CGA
TspRI         278  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       701  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcsI          130  GAATTT               [GA]^AATT[CT]
              620  AAATTC              
ApoI          130  GAATTT               [GA]^AATT[CT]
              620  AAATTC              
BbsI         1152  GAAGAC               GAAGAC(2/6)
              531  GTCTTC              
BbvI           24  GCTGC                GCAGC(8/12)
               33  GCTGC               
BmyI          808  GAGCAC               G[AGT]GC[ACT]^C
              954  GAGCCC              
BpmI          892  CTCCAG               CTGGAG(16/14)
              874  CTGGAG              
BpuAI        1152  GAAGAC               GAAGAC(2/6)
              531  GTCTTC              
BseRI         354  GAGGAG               GAGGAG(10/8)
             1102  GAGGAG              
BsmBI         463  CGTCTC               CGTCTC(1/5)
                4  GAGACG              
Bsp1286I      808  GAGCAC               G[AGT]GC[ACT]^C
              954  GAGCCC              
BspHI         401  TCATGA               T^CATGA
              753  TCATGA              
BssKI         297  CCAGG                ^CC[ACGT]GG
              869  CCTGG               
BstF5I        970  CATCC                GGATG(2/0)
             1071  GGATG               
BstNI         297  CCAGG                CC^[AT]GG
              869  CCTGG               
Cac8I          83  GCTGGC               GC[ACGT]^[ACGT]GC
              826  GCCAGC              
DpnII         193  GATC                 ^GATC
             1027  GATC                
EcoNI         570  CCTTGTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              973  CCTAAAACAGG         
EcoRII        297  CCAGG                ^CC[AT]GG
              869  CCTGG               
EcoRV         833  GATATC               GAT^ATC
              916  GATATC              
Fnu4HI         24  GCTGC                GC^[ACGT]GC
               33  GCTGC               
FokI          970  CATCC                GGATG(9/13)
             1071  GGATG               
HindIII       771  AAGCTT               A^AGCTT
             1050  AAGCTT              
HphI          606  TCACC                GGTGA(8/7)
              624  TCACC               
ItaI           24  GCTGC                GC^[ACGT]GC
               33  GCTGC               
MboI          193  GATC                 ^GATC
             1027  GATC                
MvaI          297  CCAGG                CC^[AT]GG
              869  CCTGG               
NdeII         193  GATC                 ^GATC
             1027  GATC                
NlaIV         395  GGACCC               GG[ACGT]^[ACGT]CC
              865  GGACCC              
PstI          371  CTGCAG               CTGCA^G
              536  CTGCAG              
RcaI          401  TCATGA               T^CATGA
              753  TCATGA              
Sau3AI        193  GATC                 ^GATC
             1027  GATC                
ScaI           74  AGTACT               AGT^ACT
              896  AGTACT              
ScrFI         297  CCAGG                CC^[ACGT]GG
              869  CCTGG               
SfcI          371  CTGCAG               C^T[GA][CT]AG
              536  CTGCAG              
TseI           24  GCTGC                G^C[AT]GC
               33  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        Asp700I     AvaII       BsaJI       
BsiYI       BslI        BsmAI       BsrI        Cfr13I      
DdeI        EarI        HinfI       Ksp632I     MaeII       
MboII       MnlI        MseI        MslI        MwoI        
NlaIII      RsaI        Sau96I      SfaNI       TfiI        
Tru9I       Tsp509I     XmnI        
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