Restriction map for pF1KB6986
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AviII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        MfeI        MluI        MroI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Van91I      
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

ApaI          257  GGGCCC               GGGCC^C
Asp700I       589  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         297  GGACC                G^G[AT]CC
AvrII         424  CCTAGG               C^CTAGG
BalI          149  TGGCCA               TGG^CCA
BanI          490  GGTGCC               G^G[CT][GA]CC
BbsI           25  GTCTTC               GAAGAC(2/6)
BbvI          345  GCAGC                GCAGC(8/12)
BclI          572  TGATCA               T^GATCA
BfaI          425  CTAG                 C^TAG
BglI          463  GCCTGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         182  AGATCT               A^GATCT
BlnI          424  CCTAGG               C^CTAGG
BpmI          514  CTGGAG               CTGGAG(16/14)
BpuAI          25  GTCTTC               GAAGAC(2/6)
BsaBI         243  GATAGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI         113  GAGAC                GTCTC(1/5)
BspHI         629  TCATGA               T^CATGA
BsrDI         362  GCAATG               GCAATG(2/0)
BsrFI         281  ACCGGC               [GA]^CCGG[CT]
BsrI          335  CCAGT                ACTGG(1/-1)
BstXI         560  CCATTAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         182  AGATCT               [GA]^GATC[CT]
Cfr10I        281  ACCGGC               [GA]^CCGG[CT]
DraI          657  TTTAAA               TTT^AAA
DsaI           92  CCGCGG               C^C[GA][CT]GG
EcoT14I       424  CCTAGG               C^C[AT][AT]GG
FauI          323  CCCGC                CCCGC(4/6)
FbaI          572  TGATCA               T^GATCA
Fnu4HI        345  GCAGC                GC^[ACGT]GC
ItaI          345  GCAGC                GC^[ACGT]GC
KspI           92  CCGCGG               CCGC^GG
MaeI          425  CTAG                 C^TAG
MaeII         203  ACGT                 A^CGT
MaeIII        304  GTCAC                ^GT[ACGT]AC
MamI          243  GATAGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          182  AGATCT               [GA]^GATC[CT]
MluNI         149  TGGCCA               TGG^CCA
MscI          149  TGGCCA               TGG^CCA
MspA1I         92  CCGCGG               C[AC]G^C[GT]G
PpuMI         296  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          157  CTGCAG               CTGCA^G
RcaI          629  TCATGA               T^CATGA
SacII          92  CCGCGG               CCGC^GG
SfcI          157  CTGCAG               C^T[GA][CT]AG
SmaI          260  CCCGGG               CCC^GGG
SmlI          649  CTCAAG               C^T[CT][GA]AG
StuI          222  AGGCCT               AGG^CCT
StyI          424  CCTAGG               C^C[AT][AT]GG
TseI          345  GCAGC                G^C[AT]GC
Tsp45I        304  GTCAC                ^GT[GC]AC
XhoII         182  AGATCT               [GA]^GATC[CT]
XmaCI         260  CCCGGG               C^CCGGG
XmaI          260  CCCGGG               C^CCGGG
XmnI          589  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI           60  GTAC                 GT^AC
              315  GTAC                
AluI            4  AGCT                 AG^CT
              566  AGCT                
AspI          199  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              298  GACCCGGTC           
AvaI          260  CCCGGG               C^[CT]CG[GA]G
              447  CTCGGG              
BanII          84  GGGCTC               G[GA]GC[CT]^C
              257  GGGCCC              
BsiEI           9  CGACCG               CG[GA][CT]^CG
               89  CGACCG              
BsoBI         260  CCCGGG               C^[CT]CG[GA]G
              447  CTCGGG              
BstF5I        162  GGATG                GGATG(2/0)
              195  GGATG               
Cac8I         220  GCAGGC               GC[ACGT]^[ACGT]GC
              371  GCAGGC              
DraII         256  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              296  AGGACCC             
EaeI           39  CGGCCA               [CT]^GGCC[GA]
              149  TGGCCA              
EarI           46  CTCTTC               CTCTTC(1/4)
              110  GAAGAG              
EcoO109I      256  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              296  AGGACCC             
FokI          162  GGATG                GGATG(9/13)
              195  GGATG               
Ksp632I        46  CTCTTC               CTCTTC(1/4)
              110  GAAGAG              
MseI          563  TTAA                 T^TAA
              658  TTAA                
PflFI         199  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              298  GACCCGGTC           
RsaI           60  GTAC                 GT^AC
              315  GTAC                
Tru9I         563  TTAA                 T^TAA
              658  TTAA                
Tsp509I       672  AATT                 ^AATT
              693  AATT                
Tth111I       199  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              298  GACCCGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AccII       AciI        BcnI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstNI       
BstUI       CfoI        Cfr13I      DpnII       EcoRII      
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        MboI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TspRI       TthHB8I     
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