################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BbeI BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsiEI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 955 GAATTC [GA]^AATT[CT] AcyI 114 GGCGTC G[GA]^CG[CT]C AlwNI 838 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 541 CCTCAGG CC^T[ACGT]AGG ApoI 955 GAATTC [GA]^AATT[CT] BamHI 1410 GGATCC G^GATCC BbrPI 1195 CACGTG CAC^GTG BcgI 1535 GCAATGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1353 TGATCA T^GATCA BcnI 51 CCCGG CC^[GC]GG BglII 788 AGATCT A^GATCT BsaBI 1386 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 114 GGCGTC G[GA]^CG[CT]C BsiWI 1088 CGTACG C^GTACG BsrFI 46 GCCGGC [GA]^CCGG[CT] BssSI 913 CACGAG CACGAG(-5/-1) BstAPI 138 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 972 GGTGACC G^GT[ACGT]ACC BstPI 972 GGTGACC G^GT[ACGT]ACC Bsu36I 541 CCTCAGG CC^T[ACGT]AGG Cfr10I 46 GCCGGC [GA]^CCGG[CT] Eco81I 541 CCTCAGG CC^T[ACGT]AGG EcoO65I 972 GGTGACC G^GT[ACGT]ACC EcoRI 955 GAATTC G^AATTC FbaI 1353 TGATCA T^GATCA Hin1I 114 GGCGTC G[GA]^CG[CT]C MamI 1386 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 603 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 46 GCCGGC GCC^GGC NciI 51 CCCGG CC^[GC]GG NgoMIV 46 GCCGGC G^CCGGC PmaCI 1195 CACGTG CAC^GTG PmlI 1195 CACGTG CAC^GTG PpuMI 917 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 840 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SmlI 1306 CTTGAG C^T[CT][GA]AG SphI 1148 GCATGC GCATG^C StuI 659 AGGCCT AGG^CCT TfiI 444 GAATC G^A[AT]TC XcmI 833 CCATGCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AccII 36 CGCG CG^CG 1172 CGCG ApaI 699 GGGCCC GGGCC^C 1491 GGGCCC AspHI 393 GTGCTC G[AT]GC[AT]^C 1035 GTGCTC AspI 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 202 GACGTTGTC BalI 20 TGGCCA TGG^CCA 515 TGGCCA BanI 95 GGTGCC G^G[CT][GA]CC 1505 GGTGCC BbsI 108 GAAGAC GAAGAC(2/6) 273 GAAGAC BfaI 43 CTAG C^TAG 433 CTAG BglI 26 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 853 GCCAAAGTGGC BpuAI 108 GAAGAC GAAGAC(2/6) 273 GAAGAC BsaAI 1086 CACGTA [CT]AC^GT[GA] 1195 CACGTG BsiHKAI 393 GTGCTC G[AT]GC[AT]^C 1035 GTGCTC BsmAI 1519 GAGAC GTCTC(1/5) 417 GTCTC BsmI 70 GAATGC GAATGC(1/-1) 602 GCATTC BspLU11I 302 ACATGT A^CATGT 672 ACATGT BsrDI 1549 CATTGC GCAATG(2/0) 1535 GCAATG BstUI 36 CGCG CG^CG 1172 CGCG CfoI 35 GCGC GCG^C 796 GCGC EarI 10 CTCTTC CTCTTC(1/4) 1063 GAAGAG HapII 47 CCGG C^CGG 52 CCGG HgaI 1018 GACGC GACGC(5/10) 115 GCGTC HhaI 35 GCGC GCG^C 796 GCGC HinP1I 35 GCGC G^CGC 796 GCGC HpaII 47 CCGG C^CGG 52 CCGG Ksp632I 10 CTCTTC CTCTTC(1/4) 1063 GAAGAG MaeI 43 CTAG C^TAG 433 CTAG MluNI 20 TGGCCA TGG^CCA 515 TGGCCA MscI 20 TGGCCA TGG^CCA 515 TGGCCA MseI 362 TTAA T^TAA 905 TTAA MspA1I 215 CAGCTG C[AC]G^C[GT]G 457 CAGCTG MspI 47 CCGG C^CGG 52 CCGG MvnI 36 CGCG CG^CG 1172 CGCG NdeI 192 CATATG CA^TATG 717 CATATG PflFI 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 202 GACGTTGTC PflMI 657 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1071 CCAGGAGGTGG PstI 145 CTGCAG CTGCA^G 1317 CTGCAG PvuII 215 CAGCTG CAG^CTG 457 CAGCTG SfaNI 768 GATGC GCATC(5/9) 828 GATGC SfcI 145 CTGCAG C^T[GA][CT]AG 1317 CTGCAG Tru9I 362 TTAA T^TAA 905 TTAA Tth111I 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 202 GACGTTGTC Van91I 657 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1071 CCAGGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII