Restriction map for pF1KB5013
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AfaI        
AflII       AflIII      AhdI        Alw44I      AlwI        
AlwNI       AocI        ApaI        ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DdeI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FauI        FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MflI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        TaqI        TfiI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AatII          60  GACGTC               GACGT^C
AccII          10  CGCG                 CG^CG
AcsI          525  AAATTC               [GA]^AATT[CT]
AgeI          419  ACCGGT               A^CCGGT
Aor51HI       203  AGCGCT               AGC^GCT
ApoI          525  AAATTC               [GA]^AATT[CT]
AseI          484  ATTAAT               AT^TAAT
AsnI          484  ATTAAT               AT^TAAT
Asp700I       435  GAACGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          368  TGGCCA               TGG^CCA
BanI          351  GGTGCC               G^G[CT][GA]CC
BcnI          405  CCGGG                CC^[GC]GG
BfaI          316  CTAG                 C^TAG
BmyI          352  GTGCCC               G[AGT]GC[ACT]^C
BsaWI         419  ACCGGT               [AT]^CCGG[AT]
BsgI           70  GTGCAG               GTGCAG(16/14)
Bsp1286I      352  GTGCCC               G[AGT]GC[ACT]^C
BsrI          147  CCAGT                ACTGG(1/-1)
BstF5I        237  GGATG                GGATG(2/0)
BstNI          84  CCAGG                CC^[AT]GG
BstUI          10  CGCG                 CG^CG
CfoI          204  GCGC                 GCG^C
DpnII         382  GATC                 ^GATC
DraII         311  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          113  CCACGG               C^C[GA][CT]GG
EaeI          368  TGGCCA               [CT]^GGCC[GA]
Eco47III      203  AGCGCT               AGC^GCT
EcoO109I      311  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII         84  CCAGG                ^CC[AT]GG
EcoRV         538  GATATC               GAT^ATC
FokI          237  GGATG                GGATG(9/13)
HaeII         203  AGCGCT               [GA]GCGC^[CT]
HaeIII        369  GGCC                 GG^CC
HgaI           43  GACGC                GACGC(5/10)
HhaI          204  GCGC                 GCG^C
HinP1I        204  GCGC                 G^CGC
HinfI         500  GAGTC                G^A[ACGT]TC
HphI          209  TCACC                GGTGA(8/7)
MaeI          316  CTAG                 C^TAG
MaeIII        268  GTTAC                ^GT[ACGT]AC
MboI          382  GATC                 ^GATC
MboII         535  GAAGA                GAAGA(8/7)
MluNI         368  TGGCCA               TGG^CCA
MscI          368  TGGCCA               TGG^CCA
MseI          485  TTAA                 T^TAA
MslI          210  CACCTCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           84  CCAGG                CC^[AT]GG
MvnI           10  CGCG                 CG^CG
NciI          405  CCGGG                CC^[GC]GG
NdeII         382  GATC                 ^GATC
NlaIII        543  CATG                 CATG^
PinAI         419  ACCGGT               A^CCGGT
PleI          500  GAGTC                GAGTC(4/5)
PpuMI         311  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         484  ATTAAT               AT^TAAT
Sau3AI        382  GATC                 ^GATC
SfaNI         238  GATGC                GCATC(5/9)
SspI          481  AATATT               AAT^ATT
Tru9I         485  TTAA                 T^TAA
Tsp509I       526  AATT                 ^AATT
XcmI          113  CCACGGTGGGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          435  GAACGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI           43  GACGCC               G[GA]^CG[CT]C
               60  GACGTC              
BpmI          145  CTCCAG               CTGGAG(16/14)
              591  CTCCAG              
BsaHI          43  GACGCC               G[GA]^CG[CT]C
               60  GACGTC              
BsrFI         348  GCCGGT               [GA]^CCGG[CT]
              419  ACCGGT              
BssKI          84  CCAGG                ^CC[ACGT]GG
              405  CCGGG               
Cac8I         323  GCAAGC               GC[ACGT]^[ACGT]GC
              359  GCCTGC              
Cfr10I        348  GCCGGT               [GA]^CCGG[CT]
              419  ACCGGT              
EcoT14I       391  CCTTGG               C^C[AT][AT]GG
              558  CCAAGG              
Hin1I          43  GACGCC               G[GA]^CG[CT]C
               60  GACGTC              
MaeII          61  ACGT                 A^CGT
              266  ACGT                
MspA1I        178  CAGCTG               C[AC]G^C[GT]G
              594  CAGCTG              
NlaIV         351  GGTGCC               GG[ACGT]^[ACGT]CC
              407  GGGACC              
PvuII         178  CAGCTG               CAG^CTG
              594  CAGCTG              
ScrFI          84  CCAGG                CC^[ACGT]GG
              405  CCGGG               
StyI          391  CCTTGG               C^C[AT][AT]GG
              558  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BsaJI       
BsiYI       BslI        BsmFI       Cfr13I      Fnu4HI      
HapII       HpaII       ItaI        MnlI        MspI        
MwoI        Sau96I      TseI        TspRI       
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